LOCUS       Exported               13158 bp ds-DNA     circular SYN 01-OCT-2017
DEFINITION  Gateway destination vector for expressing enhancer-driven Flp1 in 
            Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Poe AR, Tang L, Wang B, Li Y, Sapar ML, Han C.
  TITLE     Dendritic space-filling requires a neuronal type-specific 
            extracellular permissive signal in Drosophila.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 2017;114:E8062-E8071.
  PUBMED    28874572
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="synthetic DNA construct"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    632..1017
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     misc_feature    1048..2758
                     /label=Gateway RfA cassette
                     /note="Contains attR1 --> attR2"
     protein_bind    1051..1175
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1200..1230
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(1210..1232)
                     /label=ccdB_casR
                     /note="ccdB_casR"
     CDS             1284..1943
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2285..2590
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2631..2755)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     TATA_signal     2772..2974
                     /note="TATA box and 5'UTR from Hsp70Bb"
     intron          2978..3039
                     /gene="zeste"
                     /label=zIn
                     /note="2nd intron of Drosophila zeste gene"
     CDS             3059..4330
                     /label=Flippase
                     /note="Flip1 from pJFRC150-20XUAS-IVS-Flp1-PEST, encoding a
                     higher activity Flp with D at AA5."
     misc_feature    4346..4991
                     /label=His2Av stop
                     /note="His2Av stop, including 3'UTR and polyA signal"
     protein_bind    4998..5383
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     gene            5384..8751
                     /label=mini-white
     misc_feature    9529..10114
                     /label=P element 5' end
     rep_origin      complement(10349..10937)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11108..11968)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11969..12073)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    complement(12547..12616)
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttgggctgca tcacgtaata agtgtgcgtt gaatttattc
      661 gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta tcaaaaaata
      721 agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg tttttaataa
      781 aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa acaaattttc
      841 gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa atacattgca
      901 taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg ttgcataccc
      961 aataaaagat tattatattg catacctttt cttgccatac catttagccg atcaattgtg
     1021 ctcggcaaca gcatgctgca gcagatcatc acaagtttgt acaaaaaagc tgaacgagaa
     1081 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     1141 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
     1201 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     1261 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     1321 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     1381 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1441 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1501 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1561 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1621 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1681 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     1741 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1801 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1861 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1921 atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt
     1981 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     2041 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     2101 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     2161 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     2221 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     2281 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     2341 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     2401 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
     2461 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     2521 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     2581 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     2641 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     2701 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgatga
     2761 tctggtctag cgagcgccgg agtataaata gaggcgcttc gtctacggag cgacaattca
     2821 attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag
     2881 ctgagcaagc taaacaatcg taaagtgcaa gttaaagtga atcaattaaa agtaaccagc
     2941 aaccaagtaa atcaactgca actactgaaa tctgccggtg agattgtaca cccttcttaa
     3001 agatcccctt caaagtctaa attcttcctt ttcgtttagc caagactcga gaatcaaaat
     3061 gccgcagttt gatatcctct gcaagacccc accaaaggtg ttggtgcgtc aattcgtgga
     3121 gcgatttgag aggccgtcgg gtgagaagat cgccctgtgc gctgccgagt tgacctattt
     3181 gtgttggatg atcactcata atggcaccgc gattaagcgc gctaccttta tgagctataa
     3241 cactatcatt agcaattccc tgtcctttga catagtaaac aagtccctgc agtttaaata
     3301 caagactcag aaggccacta tattggaggc ttcgctgaaa aagttgatcc cggcatggga
     3361 gttcacgatc atcccatact acggtcagaa acaccagagc gatattaccg atattgtaag
     3421 cagcctccag ctgcagtttg agtccagcga agaggctgat aagggtaata gtcacagcaa
     3481 aaagatgctg aaggcactgc tgtccgaggg cgaaagcatc tgggagatta ctgagaaaat
     3541 cctgaactcg tttgagtaca ccagccgatt caccaagacg aagaccctgt accagttcct
     3601 ctttttggca accttcatca attgtggtcg cttcagtgac atcaaaaacg tggaccctaa
     3661 atcgttcaag ctggtgcaga ataagtacct gggtgttatc atccagtgct tggttaccga
     3721 gactaagacc tccgtgtcca ggcacattta tttcttctcc gcaaggggtc gcatcgatcc
     3781 gctggtatac ttggatgagt tccttcggaa tagtgaacca gtcttgaagc gcgtgaacag
     3841 gacgggcaat tccagtagca acaagcaaga gtaccagctg ctgaaggata atcttgttcg
     3901 gtcgtacaac aaagccttga agaaaaatgc gccatactcg atctttgcaa tcaagaatgg
     3961 tcccaagagt catattggcc ggcacctgat gaccagtttc ttgtccatga agggtctgac
     4021 tgagctcaca aacgtcgttg gcaactggtc ggacaagcga gcgagtgccg tcgcccgcac
     4081 gacatacacc caccagatca cggccatacc agatcactat ttcgcgctgg tgtcgcgtta
     4141 ttatgcctat gatcccatca gcaaggagat gatcgcgctg aaggacgaaa ccaatccaat
     4201 cgaggagtgg cagcatatcg agcaacttaa gggaagcgct gagggtagca tccgttaccc
     4261 cgcctggaac ggcatcatca gccaggaggt tctggattac ctgagctcct acatcaatcg
     4321 ccgtatttaa tctagaatta attaactaag ccagtcggca atcggacgcc ttcgaaacat
     4381 gcaacactaa tgtttaattc agatttcagc agagacaagc taaaacaccg acgagttgta
     4441 atcatttctg tgcgccagca tatatttctt atatacaacg taatacataa ttatgtaatt
     4501 ctagcatctc cccaacactc acatacaaac aaacaaaaaa tacaaacaca caaaacgtat
     4561 ttacccgcac gcatccttgg cgaggttgag tatgaaacaa aaacaaaact taatttagag
     4621 caaagtaatt acacgaataa atttaataaa aaaaaactat aataaaaacg ccaatcatgt
     4681 tatttccaaa aaaaaaaaaa aaaaaaaaac tagacgagat gtttgcgctt gtgtcgatga
     4741 atgtaaagaa ttgcaactac atagatggta attaatttca gctgtaagaa gtacaagttt
     4801 ttcataattg tgagaaaagt gcagtagtat cgcttaaagt tcagttttaa tcagggcatt
     4861 tagcagctcg aacccaatgt atttgtaagc ccaataaacg tgtaaatgcc tagagcaaaa
     4921 tatcacaact gtagcttttc atccgaaaga taataatgaa tagactgacc tgacgttttt
     4981 caaaatgcgt aactagtcac gtaataagtg tgcgttgaat ttattcgcaa aaacattgca
     5041 tattttcggc aaagtaaaat tttgttgcat accttatcaa aaaataagtg ctgcatactt
     5101 tttagagaaa ccaaataatt ttttattgca tacccgtttt taataaaata cattgcatac
     5161 cctcttttaa taaaaaatat tgcatacttt gacgaaacaa attttcgttg catacccaat
     5221 aaaagattat tatattgcat acccgttttt aataaaatac attgcatacc ctcttttaat
     5281 aaaaaatatt gcatacgttg acgaaacaaa ttttcgttgc atacccaata aaagattatt
     5341 atattgcata ccttttcttg ccataccatt tagccgatca attctagtat gtatgtaagt
     5401 taataaaacc cttttttgga gaatgtagat ttaaaaaaac atattttttt tttatttttt
     5461 actgcactgg atatcattga acttatctga tcagttttaa atttacttcg atccaagggt
     5521 atttgaagta ccaggttctt tcgattacct ctcactcaaa atgacattcc actcaaagtc
     5581 agcgctgttt gcctccttct ctgtccacag aaatatcgcc gtctctttcg ccgctgcgtc
     5641 cgctatctct ttcgccaccg tttgtagcgt tacctagcgt caatgtccgc cttcagttgc
     5701 actttgtcag cggtttcgtg acgaagctcc aagcggttta cgccatcaat taaacacaaa
     5761 gtgctgtgcc aaaactcctc tcgcttctta tttttgtttg ttttttgagt gattggggtg
     5821 gtgattggtt ttgggtgggt aagcagggga aagtgtgaaa aatcccggca atgggccaag
     5881 aggatcagga gctattaatt cgcggaggca gcaaacaccc atctgccgag catctgaaca
     5941 atgtgagtag tacatgtgca tacatcttaa gttcacttga tctataggaa ctgcgattgc
     6001 aacatcaaat tgtctgcggc gtgagaactg cgacccacaa aaatcccaaa ccgcaatcgc
     6061 acaaacaaat agtgacacga aacagattat tctggtagct gtgctcgcta tataagacaa
     6121 tttttaagat catatcatga tcaagacatc taaaggcatt cattttcgac tacattcttt
     6181 tttacaaaaa atataacaac cagatatttt aagctgatcc tagatgcaca aaaaataaat
     6241 aaaagtataa acctacttcg taggatactt cgttttgttc ggggttagat gagcataacg
     6301 cttgtagttg atatttgaga tcccctatca ttgcagggtg acagcggacg cttcgcagag
     6361 ctgcattaac cagggcttcg ggcaggccaa aaactacggc acgctcctgc cacccagtcc
     6421 gccggaggac tccggttcag ggagcggcca actagccgag aacctcacct atgcctggca
     6481 caatatggac atctttgggg cggtcaatca gccgggctcc ggatggcggc agctggtcaa
     6541 ccggacacgc ggactattct gcaacgagcg acacataccg gcgcccagga aacatttgct
     6601 caagaacggt gagtttctat tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc
     6661 caattaccaa tttgaaactc agtttgcggc gtggcctatc cgggcgaact tttggccgtg
     6721 atgggcagtt ccggtgccgg aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg
     6781 cagggcatcc aagtatcgcc atccgggatg cgactgctca atggccaacc tgtggacgcc
     6841 aaggagatgc aggccaggtg cgcctatgtc cagcaggatg acctctttat cggctcccta
     6901 acggccaggg aacacctgat tttccaggcc atggtgcgga tgccacgaca tctgacctat
     6961 cggcagcgag tggcccgcgt ggatcaggtg atccaggagc tttcgctcag caaatgtcag
     7021 cacacgatca tcggtgtgcc cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt
     7081 ctggcattcg cctccgaggc actaaccgat ccgccgcttc tgatctgcga tgagcccacc
     7141 tccggactgg actcatttac cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag
     7201 aagggcaaga ccgtcatcct gaccattcat cagccgtctt ccgagctgtt tgagctcttt
     7261 gacaagatcc ttctgatggc cgagggcagg gtagctttct tgggcactcc cagcgaagcc
     7321 gtcgacttct tttcctagtg agttcgatgt gtttattaag ggtatctagc attacattac
     7381 atctcaactc ctatccagcg tgggtgccca gtgtcctacc aactacaatc cggcggactt
     7441 ttacgtacag gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc
     7501 caagatatgc gacaattttg ctattagcaa agtagcccgg gatatggagc agttgttggc
     7561 caccaaaaat ttggagaagc cactggagca gccggagaat gggtacacct acaaggccac
     7621 ctggttcatg cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc
     7681 actcctcgta aaagtgcgac ttattcagac aacggtgagt ggttccagtg gaaacaaatg
     7741 atataacgct tacaattctt ggaaacaaat tcgctagatt ttagttagaa ttgcctgatt
     7801 ccacaccctt cttagttttt ttcaatgaga tgtatagttt atagttttgc agaaaataaa
     7861 taaatttcat ttaactcgcg aacatgttga agatatgaat attaatgaga tgcgagtaac
     7921 attttaattt gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca
     7981 cgcaagtggg cgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct
     8041 ttcaaaacgt ctttgccacg ataaatgtaa gtcttgttta gaatacattt gcatattaat
     8101 aatttactaa ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt
     8161 tttatgaggg aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg
     8221 attgccgaat taccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg
     8281 atgatcggac tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg
     8341 gtggccaatg tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg
     8401 atggcgctgt ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc
     8461 ttgaactcgg gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt
     8521 tacgccaacg agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc
     8581 tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac
     8641 ttctccgccg ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc
     8701 cgggtgctcg catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat
     8761 atccgaaata actgcttgtt ttttttttta ccattattac catcgtgttt actgtttatt
     8821 gccccctcaa aaagctaatg taattatatt tgtgccaata aaaacaagat atgacctata
     8881 gaatacaagt atttcccctt cgaacatccc cacaagtaga ctttggattt gtcttctaac
     8941 caaaagactt acacacctgc ataccttaca tcaaaaactc gtttatcgct acataaaaca
     9001 ccgggatata ttttttatat acatactttt caaatcgcgc gccctcttca taattcacct
     9061 ccaccacacc acgtttcgta gttgctcttt cgctgtctcc cacccgctct ccgcaacaca
     9121 ttcacctttt gttcgacgac cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc
     9181 tcaaatggtt ccgagtggtt catttcgtct caatagaaat tagtaataaa tatttgtatg
     9241 tacaatttat ttgctccaat atatttgtat atatttccct cacagctata tttattctaa
     9301 tttaatatta tgacttttta aggtaatttt ttgtgacctg ttcggagtga ttagcgttac
     9361 aatttgaact gaaagtgaca tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat
     9421 ggtgggcata atagtgttgt ttatatatat caaaaataac aactataata ataagaatac
     9481 atttaattta gaaaatgctt ggatttcact ggaactagaa ttaattcggc tgctgctcta
     9541 aacgacgcat ttcgtactcc aaagtacgaa ttttttccct caagctctta ttttcattaa
     9601 acaatgaaca ggacctaacg cacagtcacg ttattgttta cataaatgat tttttttact
     9661 attcaaactt actctgtttg tgtactccca ctggtatagc cttcttttat cttttctggt
     9721 tcaggctcta tcactttact aggtacggca tctgcgttga gtcgcctcct tttaaatgtc
     9781 tgaccttttg caggtgcagc cttccactgc gaatcattaa agtgggtatc acaaatttgg
     9841 gagttttcac caaggctgca cccaaggctc tgctcccaca attttctctt aatagcacac
     9901 ttcggcacgt gaattaattt tactccagtc acagctttgc agcaaaattt gcaatatttc
     9961 attttttttt attccacgta agggttaatg ttttcaaaaa aaaattcgtc cgcacacaac
    10021 ctttcctctc aacaagcaaa cgtgcactga atttaagtgt atacttcggt aagcttcggc
    10081 tatcgacggg accaccttat gttatttcat catgggccag acccacgtag tccagcggca
    10141 gatcggcggc ggagaagtta agcgtctcca ggatgacctt gcccgaactg gggcacgtgg
    10201 tgttcgacga tgtgcagcta atttcgcccg gctccacgtc cgcccattgg ttaatcagca
    10261 gaccctcgtt ggcgtaacgg aaccatgaga ggtacgacaa ccatttgagg tatactggca
    10321 ccgagcccga gttcaagaag aaggcgtttt tccataggct ccgcccccct gacgagcatc
    10381 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    10441 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10501 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
    10561 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10621 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    10681 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    10741 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
    10801 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10861 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10921 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10981 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    11041 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    11101 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    11161 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    11221 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    11281 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    11341 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    11401 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    11461 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    11521 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    11581 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    11641 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    11701 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
    11761 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    11821 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    11881 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    11941 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    12001 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    12061 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
    12121 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
    12181 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
    12241 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    12301 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac cattcgacat
    12361 gcccgccgtg accgtcgaga acccgctgac gctgccccgc gtatccgcac ccgccgacgc
    12421 cgtcgcacgt cccgtgctca ccgtgaccac cgcgcccagc ggtttcgagg gcgagggctt
    12481 cccggtgcgc cgcgcgttcg ccgggatcaa ctaccgccac ctcgacccgt tcatcatgat
    12541 ggaccagatg ggtgaggtgg agtacgcgcc cggggagccc aagggcacgc cctggcaccc
    12601 gcaccgcggc ttcgagaccg tgacctacat cgtcgatatg cggtgtgaaa taccgcaccg
    12661 aatcgcgcgg aactaacgac agtcgctcca aggtcgtcga acaaaaggtg aatgtgttgc
    12721 ggagagcggg tgggagacag cgaaagagca actacgaaac gtggtgtggt ggaggtgaat
    12781 tatgaagagg gcgcgcgatt tgaaaagtat gtatataaaa aatatatccc ggtgttttat
    12841 gtagcgataa acgagttttt gatgtaaggt atgcaggtgt gtaagtcttt tggttagaag
    12901 acaaatccaa agtctacttg tggggatgtt cgaaggggaa atacttgtat tctataggtc
    12961 atatcttgtt tttattggca caaatataat tacattagct ttttgagggg gcaataaaca
    13021 gtaaacacga tggtaataat ggtaaaaaaa aaaacaagca gttatttcgg atatatgtcg
    13081 gctactcctt gcgtcgggcc cgaagtctta gagccagata tgcgagcacc cggaagctca
    13141 cgatgagaat ggccagac
//
