LOCUS       Exported               14162 bp DNA     circular SYN 26-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 26, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
COMMENT     pAP-PHZICH

            pAP-ppk-hsp70TA-zIn-CD8GFP-His
FEATURES             Location/Qualifiers
     source          1..14162
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     misc_feature    240..630
                     /label=Stuffing
                     /note="Stuffing sequencing from neo gene coding region"
     protein_bind    631..1016
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     misc_feature    1047..2757
                     /label=ccdB cassette
     protein_bind    1050..1174
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1199..1229
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     misc_feature    1283..1942
                     /label=Chloramphenicol resistance gene
                     /note="Chloramphenicol resistance gene"
     CDS             1283..1942
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    2284..2589
                     /label=ccdB gene
                     /note="ccdB gene"
     CDS             2284..2589
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2630..2754)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     TATA_signal     2771..2973
                     /note="TATA box and 5'UTR from Hsp70Bb"
     intron          2977..3038
                     /note="2nd intron of zeste"
     CDS             3078..5348
                     /label=Akh-CD4-tdTom-ER
     sig_peptide     3078..3147
                     /label=Akh SP
                     /note="signal peptide of Drosophila Akh, cleaved after 
                     AA22. modified"
     J_segment       3153..3182
                     /label=Linker
                     /note="Peptide linker"
     CDS             3183..3854
                     /label=CD4-2
                     /note="CD4-2 CDS"
     misc_feature    3855..3869
                     /label=GS linker
     CDS             3876..5348
                     /label=tdTom
     CDS             3876..5303
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    5364..6009
                     /label=His2Av
                     /note="His2Av stop, including 3'UTR and polyA signal"
     3'UTR           5368..5690
                     /label=3'UTR of His2Av
                     /note="3'UTR of His2Av"
     misc_feature    5597..5617
                     /label=3'UTR screening primer
                     /note="3'UTR screening primer"
     misc_feature    5654..5659
                     /label=polyA
                     /note="polyA signal predicted by Softberry-FGENESH"
     protein_bind    6016..6401
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     promoter        6402..6880
                     /label=Pwhite
                     /note="white mini-promoter and 5'UTR "
     CDS             join(6881..6952,7354..7627,7702..8356,8418..8733,8937..9068,
                     9139..9753)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    10532..11117
                     /label=P element 5' end
     misc_feature    11118..11345
                     /label=linker
                     /note="white linker sequences"
     rep_origin      complement(11352..11940)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12111..12971)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12972..13076)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    complement(13550..13619)
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttggctgcat cacgtaataa gtgtgcgttg aatttattcg
      661 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
      721 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
      781 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
      841 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
      901 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
      961 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     1021 tcggcaacag catgctgcag cagatcatca caagtttgta caaaaaagct gaacgagaaa
     1081 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1141 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     1201 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     1261 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     1321 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     1381 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     1441 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     1501 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     1561 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     1621 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     1681 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     1741 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     1801 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     1861 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1921 tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta
     1981 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     2041 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     2101 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     2161 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     2221 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     2281 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     2341 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     2401 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     2461 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     2521 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     2581 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     2641 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     2701 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgatgat
     2761 ctggtctagc gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
     2821 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
     2881 tgagcaagct aaacaatcgt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca
     2941 accaagtaaa tcaactgcaa ctactgaaat ctgccggtga gattgtacac ccttcttaaa
     3001 gatccccttc aaagtctaaa ttcttccttt tcgtttagcc aagactcgag agaaaaccgg
     3061 tacgcgtgaa ttccaaaatg aatcccaaga gcgaagtcct cattgcagcc gtgctgttca
     3121 tgctgctggc ctgcgtccag tgtcaagcta gcggtggcgg cggaagtgga ggtggaggct
     3181 cgttccagaa ggcctccagc atagtctata agaaagaggg ggaacaggtg gagttctcct
     3241 tcccactcgc ctttacagtt gaaaagctga cgggcagtgg cgagctgtgg tggcaggcgg
     3301 agagggcttc ctcctccaag tcttggatca cctttgacct gaagaacaag gaagtgtctg
     3361 taaaacgggt tacccaggac cctaagctcc agatgggcaa gaagctcccg ctccacctca
     3421 ccctgcccca ggccttgcct cagtatgctg gctctggaaa cctcaccctg gcccttgaag
     3481 cgaaaacagg aaagttgcat caggaagtga acctggtggt gatgagagcc actcagctcc
     3541 agaaaaattt gacctgtgag gtgtggggac ccacctcccc taagctgatg ctgagcttga
     3601 aactggagaa caaggaggca aaggtctcga agcgggagaa ggcggtgtgg gtgctgaacc
     3661 ctgaggcggg gatgtggcag tgtctgctga gtgactcggg acaggtcctg ctggaatcca
     3721 acatcaaggt tctgcccaca tggtccaccc cggtgcagcc aatggccctg attgtgctgg
     3781 ggggcgtcgc cggcctcctg cttttcattg ggctaggcat cttcttctgt gtcaggtgcc
     3841 ggcaccgaag gcgcggcgga agcggaggcg gatccatggt gagcaagggc gaggaggtca
     3901 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg
     3961 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     4021 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
     4081 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
     4141 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
     4201 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
     4261 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
     4321 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
     4381 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc
     4441 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact
     4501 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg
     4561 gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg
     4621 ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg
     4681 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     4741 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca
     4801 tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt
     4861 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga
     4921 ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg
     4981 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     5041 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga
     5101 agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc
     5161 ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg
     5221 aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt
     5281 acggcatgga cgagctgtac aaggagaacg caaacagctt ttgctatgaa aatgaagttg
     5341 ccctctaatc tagaattaat taactaagcc agtcggcaat cggacgcctt cgaaacatgc
     5401 aacactaatg tttaattcag atttcagcag agacaagcta aaacaccgac gagttgtaat
     5461 catttctgtg cgccagcata tatttcttat atacaacgta atacataatt atgtaattct
     5521 agcatctccc caacactcac atacaaacaa acaaaaaata caaacacaca aaacgtattt
     5581 acccgcacgc atccttggcg aggttgagta tgaaacaaaa acaaaactta atttagagca
     5641 aagtaattac acgaataaat ttaataaaaa aaaactataa taaaaacgcc aatcatgtta
     5701 tttccaaaaa aaaaaaaaaa aaaaaaacta gacgagatgt ttgcgcttgt gtcgatgaat
     5761 gtaaagaatt gcaactacat agatggtaat taatttcagc tgtaagaagt acaagttttt
     5821 cataattgtg agaaaagtgc agtagtatcg cttaaagttc agttttaatc agggcattta
     5881 gcagctcgaa cccaatgtat ttgtaagccc aataaacgtg taaatgccta gagcaaaata
     5941 tcacaactgt agcttttcat ccgaaagata ataatgaata gactgacctg acgtttttca
     6001 aaatgcgtaa ctagtcacgt aataagtgtg cgttgaattt attcgcaaaa acattgcata
     6061 ttttcggcaa agtaaaattt tgttgcatac cttatcaaaa aataagtgct gcatactttt
     6121 tagagaaacc aaataatttt ttattgcata cccgttttta ataaaataca ttgcataccc
     6181 tcttttaata aaaaatattg catactttga cgaaacaaat tttcgttgca tacccaataa
     6241 aagattatta tattgcatac ccgtttttaa taaaatacat tgcataccct cttttaataa
     6301 aaaatattgc atacgttgac gaaacaaatt ttcgttgcat acccaataaa agattattat
     6361 attgcatacc ttttcttgcc ataccattta gccgatcaat tctagtatgt atgtaagtta
     6421 ataaaaccca tttttgcgga aagtagataa aaaaaacatt tttttttttt actgcactgg
     6481 atatcattga acttatctga tcagttttaa atttacttcg atccaagggt atttgatgta
     6541 ccaggttctt tcgattacct ctcactcaaa atgacattcc actcaaagtc agcgctgttt
     6601 gcctccttct ctgtccacag aaatatcgcc gtctctttcg ccgctgcgtc cgctatctct
     6661 ttcgccaccg tttgtagcgt tacgtagcgt caatgtccgc cttcagttgc attttgtcag
     6721 cggtttcgtg acgaagctcc aagcggttta cgccatcaat taaacacaaa gtgctgtgcc
     6781 aaaactcctc tcgcttctta tttttgtttg ttttttgagt gattggggtg gtgattggtt
     6841 ttgggtgggt aagcagggga aagtgtgaaa aatcccggca atgggccaag aggatcagga
     6901 gctattaatt cgcggaggca gcaaacaccc atctgccgag catctgaaca atgtgagtag
     6961 tacatgtgca tacatcttaa gttcacttga tctataggaa ctgcgattgc aacatcaaat
     7021 tgtctgcggc gtgagaactg cgacccacaa aaatcccaaa ccgcaatcgc acaaacaaat
     7081 agtgacacga aacagattat tctggtagct gttctcgcta tataagacaa tttttgagat
     7141 catatcatga tcaagacatc taaaggcatt cattttcgac tatattcttt tttacaaaaa
     7201 atataacaac cagatatttt aagctgatcc tagatgcaca aaaaataaat aaaagtataa
     7261 acctacttcg taggatactt cggggtactt tttgttcggg gttagatgag cataacgctt
     7321 gtagttgata tttgagatcc cctatcattg cagggtgaca gcggagcggc ttcgcagagc
     7381 tgcattaacc agggcttcgg gcaggccaaa aactacggca cgctccggcc acccagtccg
     7441 ccggaggact ccggttcagg gagcggccaa ctagccgaga acctcaccta tgcctggcac
     7501 aatatggaca tctttggggc ggtcaatcag ccgggctccg gatggcggca gctggtcaac
     7561 cggacacgcg gactattctg caacgagcga cacataccgg cgcccaggaa acatttgctc
     7621 aagaacggtg agtttctatt cgcagtcggc tgatctgtgt gaaatcttaa taaagggtcc
     7681 aattaccaat ttgaaactca gtttgcggcg tggcctatcc gggcgaactt ttggccgtga
     7741 tgggcagttc cggtgccgga aagacgaccc tgctgaatgc ccttgccttt cgatcgccgc
     7801 agggcatcca agtatcgcca tccgggatgc gactgctcaa tggccaacct gtggacgcca
     7861 aggagatgca ggccaggtgc gcctatgtcc agcaggatga cctctttatc ggctccctaa
     7921 cggccaggga acacctgatt ttccaagcca tggtgcggat gccacgacat ctgacctatc
     7981 ggcagcgagt ggcccgcgtg gatcaggtga tccaggagct ttcgctcagc aaatgtcagc
     8041 acacgatcat cggtgtgccc ggcagggtga aaggtctgtc cggcggagaa aggaagcgtc
     8101 tggcattcgc ctccgaggct ctaaccgatc cgccgcttct gatctgcgat gagcccacct
     8161 ccggactgga ctcctttacc gcccacagcg tcgtccaggt gctgaagaag ctgtcgcaga
     8221 agggcaagac cgtcatcctg accattcatc agccgtcttc cgagctgttt gagctctttg
     8281 acaagatcct tctgatggcc gagggcaggg tagctttctt gggcactccc agcgaagccg
     8341 tcgacttctt ttcctagtga gttcgatgtg tttattaagg gtatctagta ttacataaca
     8401 tctcaactcc tatccagcgt gggtgcccag tgtcctacca actacaatcc ggcggacttt
     8461 tacgtacagg tgttggccgt tgtgcccgga cgggagatcg agtcccgtga tcggatcgcc
     8521 aagatatgcg acaattttgc cattagcaaa gtagcccggg atatggagca gttgttggcc
     8581 accaaaaatc tggagaagcc actggagcag ccggagaatg ggtacaccta caaggccacc
     8641 tggttcatgc agttccgggc ggtcctgtgg cgatcctggc tgtcggtgct caaggaacca
     8701 ctcctcgtaa aagtgcgact tattcagaca acggtgagtg gttccagtgg aaacaaatga
     8761 tataacgctt acaattcttg gaaacaaatt cgctagattt tagatagaat tgcctgattc
     8821 cacacccttc ttagtttttt tcaatgagat gtatagttta tagttttgca gaagataaat
     8881 aaatttcatt taactcgcga atattaatga gatgcgagta acattttaat ttgcagatgg
     8941 ttgccatctt gattggcctc atctttttgg gccaacaact cacgcaagtg ggtgtgatga
     9001 atatcaacgg agccatcttc ctcttcctga ccaacatgac ctttcaaaac gtctttgcca
     9061 cgataaatgt aagtcatgtt tagaatacat ttgcatttca ataatttact aactttctaa
     9121 tgaatcgatt cgatttaggt gttcacctca gagctgccag tttttatgag ggaggcccga
     9181 agtcgacttt atcgctgtga cacatacttt ctgggcaaaa cgattgccga attgccgctt
     9241 tttctcacag tgccactggt cttcacggcg attgcctatc cgatgatcgg actgcgggcc
     9301 ggagtgctgc acttcttcaa ctgcctggcg ctggtcactc tggtggccaa tgtgtcaacg
     9361 tccttcggat atctaatatc ctgcgccagc tcctcgacct cgatggcgct gtctgtgggt
     9421 ccgccggtta tcataccatt cctgctcttt ggcggcttct tcttgaactc gggctcggtg
     9481 ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa cgagggtctg
     9541 ctgattaacc aatgggcgga cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc
     9601 acgtgcccca gttcgggcaa ggtcatcctg gagacgctta acttctccgc cgccgatctg
     9661 ccgctggact acgtgggtct ggccattctc atcgtgagct tccgggtgct cgcatatctg
     9721 gctctaagac ttcgggcccg acgcaaggag tagccgacat atatccgaaa taactgcttg
     9781 tttttttttt ttaccattat taccatcgtg tttactgttt attgccccct caaaaagcta
     9841 atgtaattat atttgtgcca ataaaaacaa gatatgacct atagaataca agtatttccc
     9901 cttcgaacat ccccacaagt agactttgga tttgtcttct aaccaaaaga cttacacacc
     9961 tgcatacctt acatcaaaaa ctcgtttatc gctacataaa acaccgggat atatttttta
    10021 tatacatact tttcaaatcg cgcgccctct tcataattca cctccaccac accacgtttc
    10081 gtagttgctc tttcgctgtc tcccacccgc tctccgcaac acattcacct tttgttcgac
    10141 gaccttggag cgactgtcgt tagttccgcg cgattcggtt cgctcaaatg gttccgagtg
    10201 gttcatttcg tctcaataga aattagtaat aaatatttgt atgtacaatt tatttgctcc
    10261 aatatatttg tatatatttc cctcacagct atatttattc taatttaata ttatgacttt
    10321 ttaaggtaat tttttgtgac ctgttcggag tgattagcgt tacaatttga actgaaagtg
    10381 acatccagtg tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt
    10441 tgtttatata tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg
    10501 cttggatttc actggaacta gaattaattc ggctgctgct ctaaacgacg catttcgtac
    10561 tccaaagtac gaattttttc cctcaagctc ttattttcat taaacaatga acaggaccta
    10621 acgcacagtc acgttattgt ttacataaat gatttttttt actattcaaa cttactctgt
    10681 ttgtgtactc ccactggtat agccttcttt tatcttttct ggttcaggct ctatcacttt
    10741 actaggtacg gcatctgcgt tgagtcgcct ccttttaaat gtctgacctt ttgcaggtgc
    10801 agccttccac tgcgaatcat taaagtgggt atcacaaatt tgggagtttt caccaaggct
    10861 gcacccaagg ctctgctccc acaattttct cttaatagca cacttcggca cgtgaattaa
    10921 ttttactcca gtcacagctt tgcagcaaaa tttgcaatat ttcatttttt tttattccac
    10981 gtaagggtta atgttttcaa aaaaaaattc gtccgcacac aacctttcct ctcaacaagc
    11041 aaacgtgcac tgaatttaag tgtatacttc ggtaagcttc ggctatcgac gggaccacct
    11101 tatgttattt catcatgggc cagacccacg tagtccagcg gcagatcggc ggcggagaag
    11161 ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg tggtgttcga cgatgtgcag
    11221 ctaatttcgc ccggctccac gtccgcccat tggttaatca gcagaccctc gttggcgtaa
    11281 cggaaccatg agaggtacga caaccatttg aggtatactg gcaccgagcc cgagttcaag
    11341 aagaaggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    11401 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    11461 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    11521 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    11581 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    11641 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    11701 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    11761 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
    11821 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    11881 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    11941 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    12001 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    12061 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    12121 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    12181 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    12241 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    12301 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    12361 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    12421 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    12481 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    12541 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    12601 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    12661 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    12721 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    12781 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    12841 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    12901 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    12961 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    13021 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    13081 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
    13141 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
    13201 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    13261 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
    13321 gcggcatcag agcagattgt actgagagtg caccattcga catgcccgcc gtgaccgtcg
    13381 agaacccgct gacgctgccc cgcgtatccg cacccgccga cgccgtcgca cgtcccgtgc
    13441 tcaccgtgac caccgcgccc agcggtttcg agggcgaggg cttcccggtg cgccgcgcgt
    13501 tcgccgggat caactaccgc cacctcgacc cgttcatcat gatggaccag atgggtgagg
    13561 tggagtacgc gcccggggag cccaagggca cgccctggca cccgcaccgc ggcttcgaga
    13621 ccgtgaccta catcgtcgat atgcggtgtg aaataccgca ccgaatcgcg cggaactaac
    13681 gacagtcgct ccaaggtcgt cgaacaaaag gtgaatgtgt tgcggagagc gggtgggaga
    13741 cagcgaaaga gcaactacga aacgtggtgt ggtggaggtg aattatgaag agggcgcgcg
    13801 atttgaaaag tatgtatata aaaaatatat cccggtgttt tatgtagcga taaacgagtt
    13861 tttgatgtaa ggtatgcagg tgtgtaagtc ttttggttag aagacaaatc caaagtctac
    13921 ttgtggggat gttcgaaggg gaaatacttg tattctatag gtcatatctt gtttttattg
    13981 gcacaaatat aattacatta gctttttgag ggggcaataa acagtaaaca cgatggtaat
    14041 aatggtaaaa aaaaaaaaca agcagttatt tcggatatat gtcggctact ccttgcgtcg
    14101 ggcccgaagt cttagagcca gatatgcgag cacccggaag ctcacgatga gaatggccag
    14161 ac
//
