LOCUS       pDEST-APLO              12493 bp ds-DNA     circular SYN 01-OCT-2017
DEFINITION  Gateway destination vector to express LexAop2-driven gene of 
            interest in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Poe AR, Tang L, Wang B, Li Y, Sapar ML, Han C.
  TITLE     Dendritic space-filling requires a neuronal type-specific 
            extracellular permissive signal in Drosophila.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 2017;114:E8062-E8071.
  PUBMED    28874572
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="synthetic DNA construct"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    632..1017
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     misc_feature    1031..1082
                     /label=polylinker
                     /note="polylinker"
     enhancer        1088..1466
                     /label=13XLexAop2
                     /note="13XLexAop2"
     TATA_signal     1473..1675
                     /note="TATA box and 5'UTR from Hsp70Bb"
     intron          1776..1908
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    1958..3668
                     /label=Gateway RfA cassette
                     /note="Contains attR1 --> attR2"
     protein_bind    1961..2085
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2194..2853
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3195..3500
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3541..3665)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    3681..4326
                     /label=His2Av stop
                     /note="His2Av stop, including 3'UTR and polyA signal"
     protein_bind    4333..4718
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     gene            4719..8086
                     /label=mini-white
     misc_feature    8864..9449
                     /label=P element 5' end
     rep_origin      complement(9684..10272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10443..11303)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11304..11408)
                     /gene="bla"
                     /label=AmpR promoter
     misc_recomb     11689..11971
                     /label=attB
     protein_bind    11882..11951
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttgggctgca tcacgtaata agtgtgcgtt gaatttattc
      661 gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta tcaaaaaata
      721 agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg tttttaataa
      781 aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa acaaattttc
      841 gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa atacattgca
      901 taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg ttgcataccc
      961 aataaaagat tattatattg catacctttt cttgccatac catttagccg atcaattgtg
     1021 ctcggcaaca gcatgctgca gcagatctgg tctagagccc gggcgaattc gccggcgcgc
     1081 cgagcttgca tgcctgcagg ttactgtaca tccatacagt aagtactgta catccataca
     1141 gtaagtactg tacatccata cagtaagtac tgtacatcca tacagtaagt actgtacatc
     1201 catacagtaa gcggagactc tagccctagg gcatgcctgc aggttactgt acatccatac
     1261 agtaagtact gtacatccat acagtaagta ctgtacatcc atacagtaag cggagactct
     1321 agcgctagcg catgcctgca ggttactgta catccataca gtaagtactg tacatccata
     1381 cagtaagtac tgtacatcca tacagtaagt actgtacatc catacagtaa gtactgtaca
     1441 tccatacagt aagcggagac tctagcacta gcgagcgccg gagtataaat agaggcgctt
     1501 cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc
     1561 taagcaaata aacaagcgca gctgagcaag ctaaacaatc gtaaagtgca agttaaagtg
     1621 aatcaattaa aagtaaccag caaccaagta aatcaactgc aactactgaa atctgccaag
     1681 aagtaattat tgaatacaag gagagaactc tgaatactcg accagtgact ctcttaaggt
     1741 agccttgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg
     1801 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
     1861 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc
     1921 agttcaatta cagctctcga gagaaaaccg gtacgcgatc acaagtttgt acaaaaaagc
     1981 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     2041 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
     2101 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
     2161 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     2221 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     2281 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     2341 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     2401 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     2461 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     2521 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     2581 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     2641 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     2701 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     2761 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     2821 cagtactgcg atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc
     2881 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     2941 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
     3001 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     3061 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     3121 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     3181 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     3241 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     3301 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     3361 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     3421 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     3481 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     3541 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     3601 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
     3661 gtggtgatcc tattaattaa ctaagccagt cggcaatcgg acgccttcga aacatgcaac
     3721 actaatgttt aattcagatt tcagcagaga caagctaaaa caccgacgag ttgtaatcat
     3781 ttctgtgcgc cagcatatat ttcttatata caacgtaata cataattatg taattctagc
     3841 atctccccaa cactcacata caaacaaaca aaaaatacaa acacacaaaa cgtatttacc
     3901 cgcacgcatc cttggcgagg ttgagtatga aacaaaaaca aaacttaatt tagagcaaag
     3961 taattacacg aataaattta ataaaaaaaa actataataa aaacgccaat catgttattt
     4021 ccaaaaaaaa aaaaaaaaaa aaaactagac gagatgtttg cgcttgtgtc gatgaatgta
     4081 aagaattgca actacataga tggtaattaa tttcagctgt aagaagtaca agtttttcat
     4141 aattgtgaga aaagtgcagt agtatcgctt aaagttcagt tttaatcagg gcatttagca
     4201 gctcgaaccc aatgtatttg taagcccaat aaacgtgtaa atgcctagag caaaatatca
     4261 caactgtagc ttttcatccg aaagataata atgaatagac tgacctgacg tttttcaaaa
     4321 tgcgtaacta gtcacgtaat aagtgtgcgt tgaatttatt cgcaaaaaca ttgcatattt
     4381 tcggcaaagt aaaattttgt tgcatacctt atcaaaaaat aagtgctgca tactttttag
     4441 agaaaccaaa taatttttta ttgcataccc gtttttaata aaatacattg cataccctct
     4501 tttaataaaa aatattgcat actttgacga aacaaatttt cgttgcatac ccaataaaag
     4561 attattatat tgcatacccg tttttaataa aatacattgc ataccctctt ttaataaaaa
     4621 atattgcata cgttgacgaa acaaattttc gttgcatacc caataaaaga ttattatatt
     4681 gcataccttt tcttgccata ccatttagcc gatcaattct agtatgtatg taagttaata
     4741 aaaccctttt ttggagaatg tagatttaaa aaaacatatt ttttttttat tttttactgc
     4801 actggatatc attgaactta tctgatcagt tttaaattta cttcgatcca agggtatttg
     4861 aagtaccagg ttctttcgat tacctctcac tcaaaatgac attccactca aagtcagcgc
     4921 tgtttgcctc cttctctgtc cacagaaata tcgccgtctc tttcgccgct gcgtccgcta
     4981 tctctttcgc caccgtttgt agcgttacct agcgtcaatg tccgccttca gttgcacttt
     5041 gtcagcggtt tcgtgacgaa gctccaagcg gtttacgcca tcaattaaac acaaagtgct
     5101 gtgccaaaac tcctctcgct tcttattttt gtttgttttt tgagtgattg gggtggtgat
     5161 tggttttggg tgggtaagca ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat
     5221 caggagctat taattcgcgg aggcagcaaa cacccatctg ccgagcatct gaacaatgtg
     5281 agtagtacat gtgcatacat cttaagttca cttgatctat aggaactgcg attgcaacat
     5341 caaattgtct gcggcgtgag aactgcgacc cacaaaaatc ccaaaccgca atcgcacaaa
     5401 caaatagtga cacgaaacag attattctgg tagctgtgct cgctatataa gacaattttt
     5461 aagatcatat catgatcaag acatctaaag gcattcattt tcgactacat tcttttttac
     5521 aaaaaatata acaaccagat attttaagct gatcctagat gcacaaaaaa taaataaaag
     5581 tataaaccta cttcgtagga tacttcgttt tgttcggggt tagatgagca taacgcttgt
     5641 agttgatatt tgagatcccc tatcattgca gggtgacagc ggacgcttcg cagagctgca
     5701 ttaaccaggg cttcgggcag gccaaaaact acggcacgct cctgccaccc agtccgccgg
     5761 aggactccgg ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata
     5821 tggacatctt tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga
     5881 cacgcggact attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga
     5941 acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt
     6001 accaatttga aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg
     6061 cagttccggt gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg
     6121 catccaagta tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga
     6181 gatgcaggcc aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc
     6241 cagggaacac ctgattttcc aggccatggt gcggatgcca cgacatctga cctatcggca
     6301 gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac
     6361 gatcatcggt gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc
     6421 attcgcctcc gaggcactaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg
     6481 actggactca tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg
     6541 caagaccgtc atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa
     6601 gatccttctg atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga
     6661 cttcttttcc tagtgagttc gatgtgttta ttaagggtat ctagcattac attacatctc
     6721 aactcctatc cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg
     6781 tacaggtgtt ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga
     6841 tatgcgacaa ttttgctatt agcaaagtag cccgggatat ggagcagttg ttggccacca
     6901 aaaatttgga gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt
     6961 tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc
     7021 tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata
     7081 acgcttacaa ttcttggaaa caaattcgct agattttagt tagaattgcc tgattccaca
     7141 cccttcttag tttttttcaa tgagatgtat agtttatagt tttgcagaaa ataaataaat
     7201 ttcatttaac tcgcgaacat gttgaagata tgaatattaa tgagatgcga gtaacatttt
     7261 aatttgcaga tggttgccat cttgattggc ctcatctttt tgggccaaca actcacgcaa
     7321 gtgggcgtga tgaatatcaa cggagccatc ttcctcttcc tgaccaacat gacctttcaa
     7381 aacgtctttg ccacgataaa tgtaagtctt gtttagaata catttgcata ttaataattt
     7441 actaactttc taatgaatcg attcgattta ggtgttcacc tcagagctgc cagtttttat
     7501 gagggaggcc cgaagtcgac tttatcgctg tgacacatac tttctgggca aaacgattgc
     7561 cgaattaccg ctttttctca cagtgccact ggtcttcacg gcgattgcct atccgatgat
     7621 cggactgcgg gccggagtgc tgcacttctt caactgcctg gcgctggtca ctctggtggc
     7681 caatgtgtca acgtccttcg gatatctaat atcctgcgcc agctcctcga cctcgatggc
     7741 gctgtctgtg ggtccgccgg ttatcatacc attcctgctc tttggcggct tcttcttgaa
     7801 ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac ctctcatggt tccgttacgc
     7861 caacgagggt ctgctgatta accaatgggc ggacgtggag ccgggcgaaa ttagctgcac
     7921 atcgtcgaac accacgtgcc ccagttcggg caaggtcatc ctggagacgc ttaacttctc
     7981 cgccgccgat ctgccgctgg actacgtggg tctggccatt ctcatcgtga gcttccgggt
     8041 gctcgcatat ctggctctaa gacttcgggc ccgacgcaag gagtagccga catatatccg
     8101 aaataactgc ttgttttttt ttttaccatt attaccatcg tgtttactgt ttattgcccc
     8161 ctcaaaaagc taatgtaatt atatttgtgc caataaaaac aagatatgac ctatagaata
     8221 caagtatttc cccttcgaac atccccacaa gtagactttg gatttgtctt ctaaccaaaa
     8281 gacttacaca cctgcatacc ttacatcaaa aactcgttta tcgctacata aaacaccggg
     8341 atatattttt tatatacata cttttcaaat cgcgcgccct cttcataatt cacctccacc
     8401 acaccacgtt tcgtagttgc tctttcgctg tctcccaccc gctctccgca acacattcac
     8461 cttttgttcg acgaccttgg agcgactgtc gttagttccg cgcgattcgg ttcgctcaaa
     8521 tggttccgag tggttcattt cgtctcaata gaaattagta ataaatattt gtatgtacaa
     8581 tttatttgct ccaatatatt tgtatatatt tccctcacag ctatatttat tctaatttaa
     8641 tattatgact ttttaaggta attttttgtg acctgttcgg agtgattagc gttacaattt
     8701 gaactgaaag tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa aaaatggtgg
     8761 gcataatagt gttgtttata tatatcaaaa ataacaacta taataataag aatacattta
     8821 atttagaaaa tgcttggatt tcactggaac tagaattaat tcggctgctg ctctaaacga
     8881 cgcatttcgt actccaaagt acgaattttt tccctcaagc tcttattttc attaaacaat
     8941 gaacaggacc taacgcacag tcacgttatt gtttacataa atgatttttt ttactattca
     9001 aacttactct gtttgtgtac tcccactggt atagccttct tttatctttt ctggttcagg
     9061 ctctatcact ttactaggta cggcatctgc gttgagtcgc ctccttttaa atgtctgacc
     9121 ttttgcaggt gcagccttcc actgcgaatc attaaagtgg gtatcacaaa tttgggagtt
     9181 ttcaccaagg ctgcacccaa ggctctgctc ccacaatttt ctcttaatag cacacttcgg
     9241 cacgtgaatt aattttactc cagtcacagc tttgcagcaa aatttgcaat atttcatttt
     9301 tttttattcc acgtaagggt taatgttttc aaaaaaaaat tcgtccgcac acaacctttc
     9361 ctctcaacaa gcaaacgtgc actgaattta agtgtatact tcggtaagct tcggctatcg
     9421 acgggaccac cttatgttat ttcatcatgg gccagaccca cgtagtccag cggcagatcg
     9481 gcggcggaga agttaagcgt ctccaggatg accttgcccg aactggggca cgtggtgttc
     9541 gacgatgtgc agctaatttc gcccggctcc acgtccgccc attggttaat cagcagaccc
     9601 tcgttggcgt aacggaacca tgagaggtac gacaaccatt tgaggtatac tggcaccgag
     9661 cccgagttca agaagaaggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9841 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     9901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10081 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    10141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    10201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    10261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    10321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    10381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    10441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    10501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    10561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    10621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    10681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    10741 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    10801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    10861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    10921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    10981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    11041 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
    11101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    11161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    11221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    11281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    11341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    11401 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
    11461 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    11521 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
    11581 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    11641 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc gacatgcccg
    11701 ccgtgaccgt cgagaacccg ctgacgctgc cccgcgtatc cgcacccgcc gacgccgtcg
    11761 cacgtcccgt gctcaccgtg accaccgcgc ccagcggttt cgagggcgag ggcttcccgg
    11821 tgcgccgcgc gttcgccggg atcaactacc gccacctcga cccgttcatc atgatggacc
    11881 agatgggtga ggtggagtac gcgcccgggg agcccaaggg cacgccctgg cacccgcacc
    11941 gcggcttcga gaccgtgacc tacatcgtcg atatgcggtg tgaaataccg caccgaatcg
    12001 cgcggaacta acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga
    12061 gcgggtggga gacagcgaaa gagcaactac gaaacgtggt gtggtggagg tgaattatga
    12121 agagggcgcg cgatttgaaa agtatgtata taaaaaatat atcccggtgt tttatgtagc
    12181 gataaacgag tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa
    12241 tccaaagtct acttgtgggg atgttcgaag gggaaatact tgtattctat aggtcatatc
    12301 ttgtttttat tggcacaaat ataattacat tagctttttg agggggcaat aaacagtaaa
    12361 cacgatggta ataatggtaa aaaaaaaaac aagcagttat ttcggatata tgtcggctac
    12421 tccttgcgtc gggcccgaag tcttagagcc agatatgcga gcacccggaa gctcacgatg
    12481 agaatggcca gac
//
