LOCUS       Exported                2516 bp DNA     circular SYN 21-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Han Lab
  TITLE     Direct Submission
  JOURNAL   Exported Jun 26, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    complement(268..295)
                     /label=rrnB
                     /note="rrnB T2 transcription termination sequence"
     misc_feature    complement(427..470)
                     /label=rrnB
                     /note="rrnB T1 transcription termination sequence"
     misc_feature    508..530
                     /label=O378_ENTR221_F
                     /note="O378_ENTR221_F"
     primer_bind     537..552
                     /label=M13 Forward primer
                     /note="M13 Forward primer"
     misc_feature    548..567
                     /label=assembly arm L
     primer_bind     complement(550..567)
                     /label=O398_pKrBB_R
     primer_bind     complement(550..567)
                     /label=O398
     misc_feature    579..664
                     /label=gRNA core EF
                     /note="gRNA core EF"
     misc_feature    665..670
                     /label=leader
     tRNA            671..742
                     /label=tRNA(Q)
     primer_bind     749..771
                     /label=O397_pKrBB_F
     primer_bind     749..771
                     /label=O397
     misc_feature    749..768
                     /label=assembly arm R
     promoter        complement(754..773)
                     /label=T7 promoter
                     /note="T7 promoter"
     primer_bind     complement(777..792)
                     /label=M13 reverse
     misc_feature    complement(804..825)
                     /label=O379_ENTR221_R
                     /note="O379_ENTR221_R"
     gene            910..1719
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      1840..2513
                     /direction=RIGHT
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccgct cttcacgggt ttaagagcta tgctggaaac
      601 agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      661 gtgccagcgc ggttccatgg tgtaatggtt agcactcagg actctgaatc ctgcgatccg
      721 agttcaaatc tcggtggaac ctgaggaact gatatcccct atagtgagtc gtattacatg
      781 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
      841 caagataaaa taatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
      901 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
      961 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1021 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1081 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     1141 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     1201 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     1261 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     1321 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     1381 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     1441 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     1501 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     1561 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     1621 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     1681 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     1741 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     1801 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1861 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1921 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1981 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2041 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2101 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2161 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2221 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2281 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2341 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2401 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2461 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//
