LOCUS       Exported               12778 bp ds-DNA     circular SYN 06-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12778)
  AUTHORS   Cornell University
  TITLE     Direct Submission
  JOURNAL   Exported Feb 6, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12778
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     misc_feature    132..153
                     /label=O232_GawB_P3F2
     primer_bind     222..245
                     /label=O382_pAC-P3_HiTm_R
     primer_bind     222..245
                     /label=O382_pAC-P3_HiTm_R
     primer_bind     227..245
                     /label=O382_pAC-P3_HiTm_R
                     /note="O382_pAC-P3_HiTm_R"
     misc_feature    227..233
                     /label=P3'
     misc_feature    234..285
                     /label=MCS
     misc_feature    234..246
                     /label=MCS
     primer_bind     234..246
                     /label=O789_pAC-U63_F
     primer_bind     284..312
                     /label=O509_hsp70_HiTm_F
     promoter        313..1440
                     /gene="CR34335"
                     /label=CR7T promoter
     primer_bind     complement(374..394)
                     /label=O738_CR7T_5R
     primer_bind     1076..1110
                     /label=O737_CR7T_HiTm_F
     primer_bind     1315..1337
                     /label=O736_CR7T_seqF
     primer_bind     complement(1419..1486)
                     /label=O935_EF-SapI-CR7T_R
     primer_bind     complement(1419..1440)
                     /label=O935_EF-SapI-CR7T_R
     misc_feature    1467..1562
                     /label=gRNA core 2.1
     misc_feature    1563..1678
                     /label=U6 genes 3' flanking sequence
                     /note="U6 genes 3' flanking sequence"
     primer_bind     complement(1576..1596)
                     /label=O279_pU6.3_seqR
                     /note="O279_pU6.3_seqR"
     enhancer        1685..3498
                     /label=Poly-Ubiquitin promoter
                     /note="Promoter of  Ubi-p63E; upper case indicate 5'UTR 
                     (alternative first exon)"
     enhancer        2022..2135
                     /label=Poly-Ubiquitin promoter
                     /note="Promoter of  Ubi-p63E; upper case indicate 5'UTR 
                     (alternative first exon)"
     primer_bind     complement(2943..2962)
                     /label=O796_ubiP_seq_F
     misc_signal     3516..3536
                     /label=Syn21
                     /note="Syn21 translational enhancer"
     CDS             3537..3548
                     /codon_start=1
                     /label=Start
                     /translation="MSGT"
     CDS             3549..3587
                     /codon_start=1
                     /label=NLS
                     /note="SV40 NLS"
                     /translation="DRSPPKKKRKVEF"
     CDS             3594..3623
                     /codon_start=1
                     /label=GS linker
                     /note="GS linker"
                     /translation="GGGGSGGGGS"
     CDS             3624..4334
                     /label=mTagBFP
                     /note="mTagBFP, Drosophila codon optimized"
     CDS             3624..3648
                     /codon_start=1
                     /label=mTagBFP
                     /note="mTagBFP, Drosophila codon optimized"
                     /translation="MVSKGEEL"
     primer_bind     3624..3643
                     /label=O920_TagBFP2_F
     primer_bind     complement(3668..3687)
                     /label=O795_TagBFP_seq_5R
     primer_bind     3968..3986
                     /label=O204_TagBFP_seq2_F
     primer_bind     complement(4061..4080)
                     /label=O203_TagBFP_seq1_R
     primer_bind     complement(4317..4366)
                     /label=O921_His-TagBFP2_R
     primer_bind     complement(4317..4334)
                     /label=O193_TagBFP2_KpnI_R
     primer_bind     4345..4372
                     /label=O167_His2ApA_PacI_F
     misc_feature    4353..4993
                     /label=His2Av
     3'UTR           4357..4679
                     /label=3'UTR of His2Av
                     /note="3'UTR of His2Av"
     primer_bind     complement(4442..4463)
                     /label=O4_His_5R
     primer_bind     complement(4586..4606)
                     /label=O110_His2Av_seqR
                     /note="3'UTR screening primer"
     polyA_signal    4643..4648
                     /label=polyA
                     /note="polyA signal predicted by Softberry-FGENESH"
     primer_bind     complement(4971..4993)
                     /label=O793_w-His_R
     CDS             join(5488..5559,5954..6227,6302..6956,7018..7333,7554..7685,
                     7756..8370)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    9148..9733
                     /label=P element 5' end
     misc_feature    9734..9961
                     /label=w linker
                     /note="w[+]car6.1_5', partial sequence of w last exon, 
                     inversed"
     rep_origin      complement(9968..10556)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10727..11587)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11588..11692)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    12167..12236
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    12277..12778
                     /label=w linker
                     /note="w[+]car6.1_3', patial seqence of w last exon and 3 
                     'UTR"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcagact agtggatcct taattaaggt accagatctg ctagcaaagt gaacacgtcg
      301 ctaagcgaaa gcccgttttg tcatcgcttt ttgtcgggtc tcagttgtgg atcgaaaacc
      361 cggagagtaa ccccttgacc ccttgtcagt ttccgcatgc tccagccctt gtcctcatat
      421 tttccaatcg tttttggcgt ttttagctgc ggccattgaa tgttcaaaaa ttattgtaaa
      481 cttttcagac cccctccgtg tgtgtgtgtg tgtgtgtgct tctgtgtatg tggaaaagtt
      541 cgaattctag ttgggaaatc aattaaaagc gcttgttgga ctcgcaccac aactttccct
      601 ttcgatcgcc cctgcggctt ggttcaactg gtcaaggcct ctagctcccg aaaaccgtcg
      661 aaaattggtt tggcactttt tatagccacc caatataata ccaagttcag acagaaattg
      721 atatgggtat tggactgatc aataacttaa gttccctcac tgaattatcc ttaactcttg
      781 tgcatgccta tttatgtgaa aacaatcaaa attactcaat tatgtatgca caggtattga
      841 aagctcccac tatttagttt ttgttttaat tatctgtctt tagaacttta aatggaaaaa
      901 catgacttat atgtatgaac aaaaattcca atcgatcact ttaggcggcc gccaatgcca
      961 acagaagcat tatttttttc ctttcccgat ctctcaagca cactcagttg atctcagtcg
     1021 caaaactcgt tactcactct taatatttcc tcttctttgc actttcctgg tggaatgcat
     1081 tctctatttt cctctcccta ctgcacatac tctttgagct ctctagcaga taccctaaca
     1141 atttctaatc atccacgaat aataaacaaa gatttctaat atcttaaacc tgttttatat
     1201 acttattatt caaaaaaaaa agtgtggcaa aaaaaaagag cacttcaacg tttgttgaac
     1261 aaaggatata attttgtaag ggtatctaga agttttcatg cctctcttca tccactcttc
     1321 tcaacctcat gcgatagctg ctgcgctctc tcgtttatcg ttcggttgag gttttataat
     1381 tctcaaatac tttttcccga agtggtgcct ttaaatagcg tatatgagtg gaagactttc
     1441 gcaaggaaga gcgaggctct tcttgtgttt aagagctatg ctggaaacag catagcaagt
     1501 ttaaataagg ctagtccgtt atcaactttg ctggaaacag caaagtggca ccgagtcggt
     1561 gctttttttg cctacctgga gcctgagagt tgttcaataa aataaaaatg tttcgttttt
     1621 ttgctttcgc cagtatttat tatttttcat caatatgtat tcaatttggt atgtatttac
     1681 gcgtggcttg ctgttcttcg cgttcaaaat ctccagctcc attttgcttt cggtgcgctt
     1741 gcaatcagta ctgtccaaaa tcgaaaatcg ccgaaccgta gtgtgaccgt gcggggctct
     1801 gcgaaaataa acttttttag gtatatggcc acacacgggg aaagcacagt ggattatatg
     1861 tattaatata ttatgcaggt tttcattact tatccagatg taagcccact taaagcgatt
     1921 taacaattat ttgccgaaag agtataaaca aatttcacat aaaaatggat taagaaaagc
     1981 ttgtgtaaga ttatgcgcag cgttgccaga tagctccatt taaaacactt caaaaacaat
     2041 aagtttagaa aatatataca taaatagcag tcgttgccgc aacgctcaac acatcacact
     2101 tttaaaacac cctttaccta cacagaatta ctttttaaat ttccagtcaa gctgcgagtt
     2161 tcaaaattat agccggtaga gaagacagtg ctatttcaaa agcaaactaa caagggtctt
     2221 aaatttcaaa acaccaatcc taacaagcct tggacttttg taagtttaga tcaaaggtgg
     2281 cattgcattc aatgtcatgg taagaagtag gtcgtctagg tagaaatcct cattcagccg
     2341 gtcaagtcag tacgagaaag gtctcaattt gaaattgtct taaaaatatt ttattgtttt
     2401 gtactgtggt gagtttaaac gaaaaacaca aaaaaaaagt gatacacaga aatcataaaa
     2461 aattttaata caaggtattc gtacgtatca aaaacatttc ggcacaattt tttttctctg
     2521 tactaaagtg ttacgaacac tacggtattt tttagtgatt ttcaacggac accgaaggta
     2581 tataaacagc gttcgcgaac ggtcgccttc aaaaccaatt gacatttgca gcagcaagta
     2641 caagtagaaa gtaaagcgca atcagcgaaa aatttatact taattgttgg tgattaaagt
     2701 acaattaaaa gaacattctc gaaagtcaca agaaacgtaa gtttttaact cgctgttacc
     2761 aattagtaat aagagcaaca agacgttgag taatttcaag aaaaactgca tttcaaggtc
     2821 tttgttcggc catttttttt ttattcaacg ctctacgtaa ttacaaaata agaaattggc
     2881 agccacgcat cttgttttcc caatgaattg gcatcaaaac gcaaacaaat ctataaataa
     2941 aacttgcgtg ttgattttcg ccaagattta ttggcaaatt gtgaaattcg cagtgacgca
     3001 tttgaaaatt cgagaaatca cgaacgcact cgagcatttg tgtgcatgtt attagttagt
     3061 tgtttagtta attgaagtat tttaccaacg aaatccactt atttttagct gaaatagagt
     3121 aggttgctta aacaaagcca cgtctgaaaa tttcttattg cttgtagttg tgacgtcacc
     3181 atatacacac aaaataatgt gtatgcatgc gtttcagctg tgtatatata catgcacaca
     3241 ctcgcattat gaaaacgatg acgagcaacg gaacaggttt ctcaactacc tttgttcctg
     3301 tttcttcgct ttcctttgtt ccaatattcg tagagggtta ataggggttt ctcaacaaag
     3361 ttggcgtcga taaataagtt tcccattttt attccccagc caggaagtta gtttcaatag
     3421 ttttgtaatt tcaacgaaac tcatttgatt tcgtactaat tttccacatc tctattttct
     3481 gcccgcagaa taatccaact gaatagggaa ttgggaactt aaaaaaaaaa atcaaaatga
     3541 gcggtaccga tcgcagcccc cccaagaaga agcgcaaggt ggagttcgga tccggtggcg
     3601 gcggaagtgg aggtggaggc tcgatggtgt ccaagggcga ggagctgatc aaggagaaca
     3661 tgcacatgaa gctgtacatg gagggcaccg tggacaacca ccacttcaag tgcacctccg
     3721 agggcgaggg caagccctac gagggcaccc agaccatgcg catcaaggtg gtggagggcg
     3781 gccccctgcc cttcgccttc gacatcctgg ccacctcctt cctgtacggc tccaagacct
     3841 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttcccc gagggcttca
     3901 cctgggagcg cgtgaccacc tacgaggacg gcggcgtgct gaccgccacc caggacacct
     3961 ccctgcaaga cggctgcctg atctacaacg tgaagatccg cggcgtgaac ttcacctcca
     4021 acggccccgt gatgcagaag aagaccctgg gctgggaggc cttcaccgag accctgtacc
     4081 ccgccgacgg cggcctggag ggccgcaacg acatggccct gaagctggtg ggcggctccc
     4141 acctgatcgc caacgccaag accacctacc gctccaagaa gcccgccaag aacctgaaga
     4201 tgcccggcgt gtactacgtg gactaccgcc tggagcgcat caaggaggcc aacaacgaga
     4261 cctacgtgga gcagcacgag gtggccgtgg cccgctactg cgacctgccc tccaagctgg
     4321 gccacaagct gaactaatct agaattaatt aactaagcca gtcggcaatc ggacgccttc
     4381 gaaacatgca acactaatgt ttaattcaga tttcagcaga gacaagctaa aacaccgacg
     4441 agttgtaatc atttctgtgc gccagcatat atttcttata tacaacgtaa tacataatta
     4501 tgtaattcta gcatctcccc aacactcaca tacaaacaaa caaaaaatac aaacacacaa
     4561 aacgtattta cccgcacgca tccttggcga ggttgagtat gaaacaaaaa caaaacttaa
     4621 tttagagcaa agtaattaca cgaataaatt taataaaaaa aaactataat aaaaacgcca
     4681 atcatgttat ttccaaaaaa aaaaaaaaaa aaactagacg agatgtttgc gcttgtgtcg
     4741 atgaatgtaa agaattgcaa ctacatagat ggtaattaat ttcagctgta agaagtacaa
     4801 gtttttcata attgtgagaa aagtgcagta gtatcgctta aagttcagtt ttaatcaggg
     4861 catttagcag ctcgaaccca atgtatttgt aagcccaata aacgtgtaaa tgcctagagc
     4921 aaaatatcac aactgtagct tttcatccga aagataataa tgaatagact gacctgacgt
     4981 ttttcaaaat gcgactagtg gcctaattct agtatgtatg taagttaata aaacccattt
     5041 ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata tcattgaact
     5101 tatctgatca gttttaaatt tacttcgatc caagggtatt tgatgtacca ggttctttcg
     5161 attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc tccttctctg
     5221 tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc gccaccgttt
     5281 gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg tttcgtgacg
     5341 aagctccaag cggtttacgc catcaattaa acacaaagtg ctgtgccaaa actcctctcg
     5401 cttcttattt ttgtttgttt tttgagtgat tggggtggtg attggttttg ggtgggtaag
     5461 caggggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct attaattcgc
     5521 ggaggcagca aacacccatc tgccgagcat ctgaacaatg tgagtagtac atgtgcatac
     5581 atcttaagtt cacttgatct ataggaactg cgattgcaac atcaaattgt ctgcggcgtg
     5641 agaactgcga cccacaaaaa tcccaaaccg caatcgcaca aacaaatagt gacacgaaac
     5701 agattattct ggtagctgtg ctcgctatat aagacaattt ttaagatcat atcatgatca
     5761 agacatctaa aggcattcat tttcgactac attctttttt acaaaaaata taacaaccag
     5821 atattttaag ctgatcctag atgcacaaaa aataaataaa agtataaacc tacttcgtag
     5881 gatacttcgt tttgttcggg gttagatgag cataacgctt gtagttgata tttgagatcc
     5941 cctatcattg cagggtgaca gcggagcggc ttcgcagagc tgcattaacc agggcttcgg
     6001 gcaggccaaa aactacggca cgctcctgcc acccagtccg ccggaggact ccggttcagg
     6061 gagcggccaa ctagccgaga acctcaccta tgcctggcac aatatggaca tctttggggc
     6121 ggtcaatcag ccgggctccg gatggcggca gctggtcaac cggacacgcg gactattctg
     6181 caacgagcga cacataccgg cgcccaggaa acatttgctc aagaacggtg agtttctatt
     6241 cgcagtcggc tgatctgtgt gaaatcttaa taaagggtcc aattaccaat ttgaaactca
     6301 gtttgcggcg tggcctatcc gggcgaactt ttggccgtga tgggcagttc cggtgccgga
     6361 aagacgaccc tgctgaatgc ccttgccttt cgatcgccgc agggcatcca agtatcgcca
     6421 tccgggatgc gactgctcaa tggccaacct gtggacgcca aggagatgca ggccaggtgc
     6481 gcctatgtcc agcaggatga cctctttatc ggctccctaa cggccaggga acacctgatt
     6541 ttccaggcca tggtgcggat gccacgacat ctgacctatc ggcagcgagt ggcccgcgtg
     6601 gatcaggtga tccaggagct ttcgctcagc aaatgtcagc acacgatcat cggtgtgccc
     6661 ggcagggtga aaggtctgtc cggcggagaa aggaagcgtc tggcattcgc ctccgaggca
     6721 ctaaccgatc cgccgcttct gatctgcgat gagcccacct ccggactgga ctcatttacc
     6781 gcccacagcg tcgtccaggt gctgaagaag ctgtcgcaga agggcaagac cgtcatcctg
     6841 accattcatc agccgtcttc cgagctgttt gagctctttg acaagatcct tctgatggcc
     6901 gagggcaggg tagctttctt gggcactccc agcgaagccg tcgacttctt ttcctagtga
     6961 gttcgatgtg tttattaagg gtatctagca ttacattaca tctcaactcc tatccagcgt
     7021 gggtgcccag tgtcctacca actacaatcc ggcggacttt tacgtacagg tgttggccgt
     7081 tgtgcccgga cgggagatcg agtcccgtga tcggatcgcc aagatatgcg acaattttgc
     7141 tattagcaaa gtagcccggg atatggagca gttgttggcc accaaaaatt tggagaagcc
     7201 actggagcag ccggagaatg ggtacaccta caaggccacc tggttcatgc agttccgggc
     7261 ggtcctgtgg cgatcctggc tgtcggtgct caaggaacca ctcctcgtaa aagtgcgact
     7321 tattcagaca acggtgagtg gttccagtgg aaacaaatga tataacgctt acaattcttg
     7381 gaaacaaatt cgctagattt tagttagaat tgcctgattc cacacccttc ttagtttttt
     7441 tcaatgagat gtatagttta tagttttgca gaaaataaat aaatttcatt taactcgcga
     7501 acatgttgaa gatatgaata ttaatgagat gcgagtaaca ttttaatttg cagatggttg
     7561 ccatcttgat tggcctcatc tttttgggcc aacaactcac gcaagtgggc gtgatgaata
     7621 tcaacggagc catcttcctc ttcctgacca acatgacctt tcaaaacgtc tttgccacga
     7681 taaatgtaag tcttgtttag aatacatttg catattaata atttactaac tttctaatga
     7741 atcgattcga tttaggtgtt cacctcagag ctgccagttt ttatgaggga ggcccgaagt
     7801 cgactttatc gctgtgacac atactttctg ggcaaaacga ttgccgaatt accgcttttt
     7861 ctcacagtgc cactggtctt cacggcgatt gcctatccga tgatcggact gcgggccgga
     7921 gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc
     7981 ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg
     8041 ccggttatca taccattcct gctctttggc ggcttcttct tgaactcggg ctcggtgcca
     8101 gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg
     8161 attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg
     8221 tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc cgatctgccg
     8281 ctggactacg tgggtctggc cattctcatc gtgagcttcc gggtgctcgc atatctggct
     8341 ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa ctgcttgttt
     8401 ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt
     8461 aattatattt gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc
     8521 gaacatcccc acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca
     8581 taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata
     8641 catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag
     8701 ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc
     8761 ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc
     8821 atttcgtctc aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata
     8881 tatttgtata tatttccctc acagctatat ttattctaat ttaatattat gactttttaa
     8941 ggtaattttt tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat
     9001 ccagtgtttg ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt
     9061 tatatatatc aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg
     9121 gatttcactg gaactagaat taattcggct gctgctctaa acgacgcatt tcgtactcca
     9181 aagtacgaat tttttccctc aagctcttat tttcattaaa caatgaacag gacctaacgc
     9241 acagtcacgt tattgtttac ataaatgatt ttttttacta ttcaaactta ctctgtttgt
     9301 gtactcccac tggtatagcc ttcttttatc ttttctggtt caggctctat cactttacta
     9361 ggtacggcat ctgcgttgag tcgcctcctt ttaaatgtct gaccttttgc aggtgcagcc
     9421 ttccactgcg aatcattaaa gtgggtatca caaatttggg agttttcacc aaggctgcac
     9481 ccaaggctct gctcccacaa ttttctctta atagcacact tcggcacgtg aattaatttt
     9541 actccagtca cagctttgca gcaaaatttg caatatttca ttttttttta ttccacgtaa
     9601 gggttaatgt tttcaaaaaa aaattcgtcc gcacacaacc tttcctctca acaagcaaac
     9661 gtgcactgaa tttaagtgta tacttcggta agcttcggct atcgacggga ccaccttatg
     9721 ttatttcatc atgggccaga cccacgtagt ccagcggcag atcggcggcg gagaagttaa
     9781 gcgtctccag gatgaccttg cccgaactgg ggcacgtggt gttcgacgat gtgcagctaa
     9841 tttcgcccgg ctccacgtcc gcccattggt taatcagcag accctcgttg gcgtaacgga
     9901 accatgagag gtacgacaac catttgaggt atactggcac cgagcccgag ttcaagaaga
     9961 aggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    10021 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    10081 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10141 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
    10201 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    10261 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10321 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10381 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    10441 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10501 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10561 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10621 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10681 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10741 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10801 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10861 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10921 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10981 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    11041 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    11101 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    11161 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    11221 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    11281 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    11341 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    11401 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11461 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11521 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11581 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11641 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11701 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    11761 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    11821 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    11881 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    11941 catcagagca gattgtactg agagtgcacc atatcgacat gcccgccgtg accgtcgaga
    12001 acccgctgac gctgccccgc gtatccgcac ccgccgacgc cgtcgcacgt cccgtgctca
    12061 ccgtgaccac cgcgcccagc ggtttcgagg gcgagggctt cccggtgcgc cgcgcgttcg
    12121 ccgggatcaa ctaccgccac ctcgacccgt tcatcatgat ggaccagatg ggtgaggtgg
    12181 agtacgcgcc cggggagccc aagggcacgc cctggcaccc gcaccgcggc ttcgagaccg
    12241 tgacctacat cgtcgatatg cggtgtgaaa taccgcaccg aatcgcgcgg aactaacgac
    12301 agtcgctcca aggtcgtcga acaaaaggtg aatgtgttgc ggagagcggg tgggagacag
    12361 cgaaagagca actacgaaac gtggtgtggt ggaggtgaat tatgaagagg gcgcgcgatt
    12421 tgaaaagtat gtatataaaa aatatatccc ggtgttttat gtagcgataa acgagttttt
    12481 gatgtaaggt atgcaggtgt gtaagtcttt tggttagaag acaaatccaa agtctacttg
    12541 tggggatgtt cgaaggggaa atacttgtat tctataggtc atatcttgtt tttattggca
    12601 caaatataat tacattagct ttttgagggg gcaataaaca gtaaacacga tggtaataat
    12661 ggtaaaaaaa aaaacaagca gttatttcgg atatatgtcg gctactcctt gcgtcgggcc
    12721 cgaagtctta gagccagata tgcgagcacc cggaagctca cgatgagaat ggccagac
//
