LOCUS       Exported               12230 bp ds-DNA     circular SYN 09-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12230)
  AUTHORS   Han Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 9, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12230
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     misc_feature    234..263
                     /label=MCS
     promoter        264..681
                     /label=U6:3 promoter
                     /note="U6:3 promoter"
     tRNA            682..778
                     /note="Dm tRNA Gly"
     misc_feature    779..803
                     /label=SapI adaptor
                     /note="SapI adaptor"
     misc_feature    804..889
                     /label=gRNA core EF
                     /note="gRNA core EF"
     misc_feature    890..1124
                     /label=U6 genes 3' flanking sequence
                     /note="U6 genes 3' flanking sequence"
     enhancer        1137..2950
                     /label=Poly-Ubiquitin promoter
                     /note="Promoter of Ubi-p63E; upper case indicate 5'UTR 
                     (alternative first exon)"
     enhancer        1474..1587
                     /label=Poly-Ubiquitin promoter
                     /note="Promoter of Ubi-p63E; upper case indicate 5'UTR 
                     (alternative first exon)"
     misc_signal     2968..2988
                     /label=Syn21
                     /note="Syn21 translational enhancer"
     CDS             2989..3000
                     /codon_start=1
                     /label=Start
                     /translation="MSGT"
     CDS             3001..3039
                     /codon_start=1
                     /label=NLS
                     /note="SV40 NLS"
                     /translation="DRSPPKKKRKVEF"
     CDS             3046..3075
                     /codon_start=1
                     /label=GS linker
                     /note="GS linker"
                     /translation="GGGGSGGGGS"
     CDS             3076..3786
                     /label=mTagBFP
                     /note="mTagBFP, Drosophila codon optimized"
     misc_feature    3805..4445
                     /label=His2Av
     3'UTR           3809..4131
                     /label=3'UTR of His2Av
                     /note="3'UTR of His2Av"
     polyA_signal    4095..4100
                     /label=polyA
                     /note="polyA signal predicted by Softberry-FGENESH"
     CDS             join(4940..5011,5406..5679,5754..6408,6470..6785,7006..7137,
                     7208..7822)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    8600..9185
                     /label=P element 5' end
     misc_feature    9186..9413
                     /label=w linker
                     /note="w[+]car6.1_5', partial sequence of w last exon, 
                     inversed"
     rep_origin      complement(9420..10008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10179..11039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11040..11144)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    11619..11688
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    11729..12230
                     /label=w linker
                     /note="w[+]car6.1_3', patial seqence of w last exon and 3 
                     'UTR"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcagtcg acgctagcat atgtttgctc acctgtgatt gctcctactc aaatacaaaa
      301 acatcaaatt ttctgtcaat aaagcatatt tatttatatt tattttacag gaaagaattc
      361 cttttaaagt gtattttaac ctataatgaa aaacgattaa aaaaaataca taaaataatt
      421 cgaaaatttt tgaatagccc aggttgataa aaattcattt catacgtttt ataacttatg
      481 cccctaagta ttttttgacc atagtgtttc aattctacat taattttaca gagtagaatg
      541 aaacgccacc tactcagcca agaggcgaaa aggttagctc gccaagcaga gagggcgcca
      601 gtgctcacta ctttttataa ttctcaactt ctttttccag actcagttcg tatatataga
      661 cctattttca atttaacgtc ggggctttga gtgtgtgtag acatcaagca tcggtggttc
      721 agtggtagaa tgctcgcctg ccacgcgggc ggcccgggtt cgattcccgg ccgatgcaca
      781 aggaagagcg aggctcttct tgtgtttaag agctatgctg gaaacagcat agcaagttta
      841 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttgcct
      901 acctggagcc tgagagttgt tcaataaaat aaaaatgttt cgtttttttg ctttcgccag
      961 tatttattat ttttcatcaa tatgtattca atttggtatg tatttagtaa ttgtaatata
     1021 tagacaatgg ttttccgttg acgtacatac atctgacgtg tgtttattta gacataatag
     1081 ttatgttttc acatcttttt aatgttcgct taatgcgtat gcattctagt ggccatggct
     1141 tgctgttctt cgcgttcaaa atctccagct ccattttgct ttcggtgcgc ttgcaatcag
     1201 tactgtccaa aatcgaaaat cgccgaaccg tagtgtgacc gtgcggggct ctgcgaaaat
     1261 aaactttttt aggtatatgg ccacacacgg ggaaagcaca gtggattata tgtattaata
     1321 tattatgcag gttttcatta cttatccaga tgtaagccca cttaaagcga tttaacaatt
     1381 atttgccgaa agagtataaa caaatttcac ataaaaatgg attaagaaaa gcttgtgtaa
     1441 gattatgcgc agcgttgcca gatagctcca tttaaaacac ttcaaaaaca ataagtttag
     1501 aaaatatata cataaatagc agtcgttgcc gcaacgctca acacatcaca cttttaaaac
     1561 accctttacc tacacagaat tactttttaa atttccagtc aagctgcgag tttcaaaatt
     1621 atagccggta gagaagacag tgctatttca aaagcaaact aacaagggtc ttaaatttca
     1681 aaacaccaat cctaacaagc cttggacttt tgtaagttta gatcaaaggt ggcattgcat
     1741 tcaatgtcat ggtaagaagt aggtcgtcta ggtagaaatc ctcattcagc cggtcaagtc
     1801 agtacgagaa aggtctcaat ttgaaattgt cttaaaaata ttttattgtt ttgtactgtg
     1861 gtgagtttaa acgaaaaaca caaaaaaaaa gtgatacaca gaaatcataa aaaattttaa
     1921 tacaaggtat tcgtacgtat caaaaacatt tcggcacaat tttttttctc tgtactaaag
     1981 tgttacgaac actacggtat tttttagtga ttttcaacgg acaccgaagg tatataaaca
     2041 gcgttcgcga acggtcgcct tcaaaaccaa ttgacatttg cagcagcaag tacaagtaga
     2101 aagtaaagcg caatcagcga aaaatttata cttaattgtt ggtgattaaa gtacaattaa
     2161 aagaacattc tcgaaagtca caagaaacgt aagtttttaa ctcgctgtta ccaattagta
     2221 ataagagcaa caagacgttg agtaatttca agaaaaactg catttcaagg tctttgttcg
     2281 gccatttttt ttttattcaa cgctctacgt aattacaaaa taagaaattg gcagccacgc
     2341 atcttgtttt cccaatgaat tggcatcaaa acgcaaacaa atctataaat aaaacttgcg
     2401 tgttgatttt cgccaagatt tattggcaaa ttgtgaaatt cgcagtgacg catttgaaaa
     2461 ttcgagaaat cacgaacgca ctcgagcatt tgtgtgcatg ttattagtta gttgtttagt
     2521 taattgaagt attttaccaa cgaaatccac ttatttttag ctgaaataga gtaggttgct
     2581 taaacaaagc cacgtctgaa aatttcttat tgcttgtagt tgtgacgtca ccatatacac
     2641 acaaaataat gtgtatgcat gcgtttcagc tgtgtatata tacatgcaca cactcgcatt
     2701 atgaaaacga tgacgagcaa cggaacaggt ttctcaacta cctttgttcc tgtttcttcg
     2761 ctttcctttg ttccaatatt cgtagagggt taataggggt ttctcaacaa agttggcgtc
     2821 gataaataag tttcccattt ttattcccca gccaggaagt tagtttcaat agttttgtaa
     2881 tttcaacgaa actcatttga tttcgtacta attttccaca tctctatttt ctgcccgcag
     2941 aataatccaa ctgaataggg aattgggaac ttaaaaaaaa aaatcaaaat gagcggtacc
     3001 gatcgcagcc cccccaagaa gaagcgcaag gtggagttcg gatccggtgg cggcggaagt
     3061 ggaggtggag gctcgatggt gtccaagggc gaggagctga tcaaggagaa catgcacatg
     3121 aagctgtaca tggagggcac cgtggacaac caccacttca agtgcacctc cgagggcgag
     3181 ggcaagccct acgagggcac ccagaccatg cgcatcaagg tggtggaggg cggccccctg
     3241 cccttcgcct tcgacatcct ggccacctcc ttcctgtacg gctccaagac cttcatcaac
     3301 cacacccagg gcatccccga cttcttcaag cagtccttcc ccgagggctt cacctgggag
     3361 cgcgtgacca cctacgagga cggcggcgtg ctgaccgcca cccaggacac ctccctgcaa
     3421 gacggctgcc tgatctacaa cgtgaagatc cgcggcgtga acttcacctc caacggcccc
     3481 gtgatgcaga agaagaccct gggctgggag gccttcaccg agaccctgta ccccgccgac
     3541 ggcggcctgg agggccgcaa cgacatggcc ctgaagctgg tgggcggctc ccacctgatc
     3601 gccaacgcca agaccaccta ccgctccaag aagcccgcca agaacctgaa gatgcccggc
     3661 gtgtactacg tggactaccg cctggagcgc atcaaggagg ccaacaacga gacctacgtg
     3721 gagcagcacg aggtggccgt ggcccgctac tgcgacctgc cctccaagct gggccacaag
     3781 ctgaactaat ctagaattaa ttaactaagc cagtcggcaa tcggacgcct tcgaaacatg
     3841 caacactaat gtttaattca gatttcagca gagacaagct aaaacaccga cgagttgtaa
     3901 tcatttctgt gcgccagcat atatttctta tatacaacgt aatacataat tatgtaattc
     3961 tagcatctcc ccaacactca catacaaaca aacaaaaaat acaaacacac aaaacgtatt
     4021 tacccgcacg catccttggc gaggttgagt atgaaacaaa aacaaaactt aatttagagc
     4081 aaagtaatta cacgaataaa tttaataaaa aaaaactata ataaaaacgc caatcatgtt
     4141 atttccaaaa aaaaaaaaaa aaaaactaga cgagatgttt gcgcttgtgt cgatgaatgt
     4201 aaagaattgc aactacatag atggtaatta atttcagctg taagaagtac aagtttttca
     4261 taattgtgag aaaagtgcag tagtatcgct taaagttcag ttttaatcag ggcatttagc
     4321 agctcgaacc caatgtattt gtaagcccaa taaacgtgta aatgcctaga gcaaaatatc
     4381 acaactgtag cttttcatcc gaaagataat aatgaataga ctgacctgac gtttttcaaa
     4441 atgcgactag tggcctaatt ctagtatgta tgtaagttaa taaaacccat ttttgcggaa
     4501 agtagataaa aaaaacattt ttttttttta ctgcactgga tatcattgaa cttatctgat
     4561 cagttttaaa tttacttcga tccaagggta tttgatgtac caggttcttt cgattacctc
     4621 tcactcaaaa tgacattcca ctcaaagtca gcgctgtttg cctccttctc tgtccacaga
     4681 aatatcgccg tctctttcgc cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt
     4741 acgtagcgtc aatgtccgcc ttcagttgca ttttgtcagc ggtttcgtga cgaagctcca
     4801 agcggtttac gccatcaatt aaacacaaag tgctgtgcca aaactcctct cgcttcttat
     4861 ttttgtttgt tttttgagtg attggggtgg tgattggttt tgggtgggta agcaggggaa
     4921 agtgtgaaaa atcccggcaa tgggccaaga ggatcaggag ctattaattc gcggaggcag
     4981 caaacaccca tctgccgagc atctgaacaa tgtgagtagt acatgtgcat acatcttaag
     5041 ttcacttgat ctataggaac tgcgattgca acatcaaatt gtctgcggcg tgagaactgc
     5101 gacccacaaa aatcccaaac cgcaatcgca caaacaaata gtgacacgaa acagattatt
     5161 ctggtagctg tgctcgctat ataagacaat ttttaagatc atatcatgat caagacatct
     5221 aaaggcattc attttcgact acattctttt ttacaaaaaa tataacaacc agatatttta
     5281 agctgatcct agatgcacaa aaaataaata aaagtataaa cctacttcgt aggatacttc
     5341 gttttgttcg gggttagatg agcataacgc ttgtagttga tatttgagat cccctatcat
     5401 tgcagggtga cagcggagcg gcttcgcaga gctgcattaa ccagggcttc gggcaggcca
     5461 aaaactacgg cacgctcctg ccacccagtc cgccggagga ctccggttca gggagcggcc
     5521 aactagccga gaacctcacc tatgcctggc acaatatgga catctttggg gcggtcaatc
     5581 agccgggctc cggatggcgg cagctggtca accggacacg cggactattc tgcaacgagc
     5641 gacacatacc ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg
     5701 gctgatctgt gtgaaatctt aataaagggt ccaattacca atttgaaact cagtttgcgg
     5761 cgtggcctat ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac
     5821 cctgctgaat gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat
     5881 gcgactgctc aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt
     5941 ccagcaggat gacctcttta tcggctccct aacggccagg gaacacctga ttttccaggc
     6001 catggtgcgg atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt
     6061 gatccaggag ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt
     6121 gaaaggtctg tccggcggag aaaggaagcg tctggcattc gcctccgagg cactaaccga
     6181 tccgccgctt ctgatctgcg atgagcccac ctccggactg gactcattta ccgcccacag
     6241 cgtcgtccag gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca
     6301 tcagccgtct tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag
     6361 ggtagctttc ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg
     6421 tgtttattaa gggtatctag cattacatta catctcaact cctatccagc gtgggtgccc
     6481 agtgtcctac caactacaat ccggcggact tttacgtaca ggtgttggcc gttgtgcccg
     6541 gacgggagat cgagtcccgt gatcggatcg ccaagatatg cgacaatttt gctattagca
     6601 aagtagcccg ggatatggag cagttgttgg ccaccaaaaa tttggagaag ccactggagc
     6661 agccggagaa tgggtacacc tacaaggcca cctggttcat gcagttccgg gcggtcctgt
     6721 ggcgatcctg gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga cttattcaga
     6781 caacggtgag tggttccagt ggaaacaaat gatataacgc ttacaattct tggaaacaaa
     6841 ttcgctagat tttagttaga attgcctgat tccacaccct tcttagtttt tttcaatgag
     6901 atgtatagtt tatagttttg cagaaaataa ataaatttca tttaactcgc gaacatgttg
     6961 aagatatgaa tattaatgag atgcgagtaa cattttaatt tgcagatggt tgccatcttg
     7021 attggcctca tctttttggg ccaacaactc acgcaagtgg gcgtgatgaa tatcaacgga
     7081 gccatcttcc tcttcctgac caacatgacc tttcaaaacg tctttgccac gataaatgta
     7141 agtcttgttt agaatacatt tgcatattaa taatttacta actttctaat gaatcgattc
     7201 gatttaggtg ttcacctcag agctgccagt ttttatgagg gaggcccgaa gtcgacttta
     7261 tcgctgtgac acatactttc tgggcaaaac gattgccgaa ttaccgcttt ttctcacagt
     7321 gccactggtc ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca
     7381 cttcttcaac tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt ccttcggata
     7441 tctaatatcc tgcgccagct cctcgacctc gatggcgctg tctgtgggtc cgccggttat
     7501 cataccattc ctgctctttg gcggcttctt cttgaactcg ggctcggtgc cagtatacct
     7561 caaatggttg tcgtacctct catggttccg ttacgccaac gagggtctgc tgattaacca
     7621 atgggcggac gtggagccgg gcgaaattag ctgcacatcg tcgaacacca cgtgccccag
     7681 ttcgggcaag gtcatcctgg agacgcttaa cttctccgcc gccgatctgc cgctggacta
     7741 cgtgggtctg gccattctca tcgtgagctt ccgggtgctc gcatatctgg ctctaagact
     7801 tcgggcccga cgcaaggagt agccgacata tatccgaaat aactgcttgt tttttttttt
     7861 accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat
     7921 ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc
     7981 ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac
     8041 atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata tacatacttt
     8101 tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt agttgctctt
     8161 tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga ccttggagcg
     8221 actgtcgtta gttccgcgcg attcggttcg ctcaaatggt tccgagtggt tcatttcgtc
     8281 tcaatagaaa ttagtaataa atatttgtat gtacaattta tttgctccaa tatatttgta
     8341 tatatttccc tcacagctat atttattcta atttaatatt atgacttttt aaggtaattt
     8401 tttgtgacct gttcggagtg attagcgtta caatttgaac tgaaagtgac atccagtgtt
     8461 tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg tttatatata
     8521 tcaaaaataa caactataat aataagaata catttaattt agaaaatgct tggatttcac
     8581 tggaactaga attaattcgg ctgctgctct aaacgacgca tttcgtactc caaagtacga
     8641 attttttccc tcaagctctt attttcatta aacaatgaac aggacctaac gcacagtcac
     8701 gttattgttt acataaatga ttttttttac tattcaaact tactctgttt gtgtactccc
     8761 actggtatag ccttctttta tcttttctgg ttcaggctct atcactttac taggtacggc
     8821 atctgcgttg agtcgcctcc ttttaaatgt ctgacctttt gcaggtgcag ccttccactg
     8881 cgaatcatta aagtgggtat cacaaatttg ggagttttca ccaaggctgc acccaaggct
     8941 ctgctcccac aattttctct taatagcaca cttcggcacg tgaattaatt ttactccagt
     9001 cacagctttg cagcaaaatt tgcaatattt catttttttt tattccacgt aagggttaat
     9061 gttttcaaaa aaaaattcgt ccgcacacaa cctttcctct caacaagcaa acgtgcactg
     9121 aatttaagtg tatacttcgg taagcttcgg ctatcgacgg gaccacctta tgttatttca
     9181 tcatgggcca gacccacgta gtccagcggc agatcggcgg cggagaagtt aagcgtctcc
     9241 aggatgacct tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct aatttcgccc
     9301 ggctccacgt ccgcccattg gttaatcagc agaccctcgt tggcgtaacg gaaccatgag
     9361 aggtacgaca accatttgag gtatactggc accgagcccg agttcaagaa gaaggcgttt
     9421 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9481 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9541 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9601 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9661 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9721 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9781 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9841 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9901 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9961 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10021 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    10081 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10141 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10201 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10261 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10321 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10381 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10441 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10501 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10561 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10621 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10681 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10741 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
    10801 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10861 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10921 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10981 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    11041 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    11101 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    11161 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    11221 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    11281 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    11341 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
    11401 cagattgtac tgagagtgca ccatatcgac atgcccgccg tgaccgtcga gaacccgctg
    11461 acgctgcccc gcgtatccgc acccgccgac gccgtcgcac gtcccgtgct caccgtgacc
    11521 accgcgccca gcggtttcga gggcgagggc ttcccggtgc gccgcgcgtt cgccgggatc
    11581 aactaccgcc acctcgaccc gttcatcatg atggaccaga tgggtgaggt ggagtacgcg
    11641 cccggggagc ccaagggcac gccctggcac ccgcaccgcg gcttcgagac cgtgacctac
    11701 atcgtcgata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg acagtcgctc
    11761 caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac agcgaaagag
    11821 caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga tttgaaaagt
    11881 atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt ttgatgtaag
    11941 gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact tgtggggatg
    12001 ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg cacaaatata
    12061 attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata atggtaaaaa
    12121 aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg cccgaagtct
    12181 tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
//
