LOCUS       Exported               16062 bp ds-DNA     circular SYN 19-JUL-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16062)
  AUTHORS   Chun Han
  TITLE     Direct Submission
  JOURNAL   Exported Feb 28, 2018 from SnapGene 4.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16062
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..239
                     /label=P3'
                     /note="P element 3' end"
     misc_feature    240..630
                     /label=Stuffing
                     /note="Stuffing sequencing from neo gene coding region"
     misc_feature    631..1026
                     /label=in
                     /note="gypsy insulator"
     misc_feature    1035..3041
                     /label=pDEST-APIH-Akh-CD4-tdGFP2-ER1
     misc_feature    1048..2758
                     /label=ccdB RfA
                     /note="ccdB cassette reading frame A"
     misc_feature    1051..1175
                     /label=attR1
                     /note="attR1 site"
     misc_feature    complement(1210..1232)
                     /label=ccdB_casR
                     /note="ccdB_casR"
     misc_feature    1284..1943
                     /label=Chloramphenicol resistance gene
                     /note="Chloramphenicol resistance gene"
     misc_feature    complement(1352..1373)
                     /label=ccdB_casRII
     misc_feature    2285..2590
                     /label=ccdB gene
                     /note="ccdB gene"
     misc_feature    2405..2426
                     /label=ccdB_casFII
     misc_feature    2491..2513
                     /label=ccdB_casF
                     /note="ccdB_casF"
     misc_feature    2631..2755
                     /label=attR2
                     /note="attR2 site"
     promoter        2772..2974
                     /label=DSCPm
                     /note="DSCPm"
     TATA_signal     2772..2974
                     /label=Hsp70
                     /note="TATA box and 5'UTR from Hsp70Bb"
     misc_feature    2783..2788
                     /label=TATA
                     /note="TATA box"
     misc_feature    2812..2817
                     /label=Inr
                     /note="Inr. A is the transcription starting site (TSS)"
     misc_feature    2831..2840
                     /label=MTE
                     /note="MTE"
     misc_feature    2841..2846
                     /label=DPE
                     /note="DPE"
     misc_feature    2957..2976
                     /label=O1_5UAST
                     /note="O1_5UAST"
     intron          2978..3039
                     /label=zeste 2nd intron
                     /note="zeste 2nd intron"
     intron          3026..3039
                     /note="2nd intron of zeste"
     primer_bind     3030..3072
                     /label=O269_HybIn-Cas9_F
     CDS             3055..7260
                     /label=Cas9-2xNLS CDS
                     /note="Also used by Jianquan Ni to make nos-Cas9"
     misc_feature    3055..3105
                     /label=NLS
                     /note="NLS"
     CDS             3106..7209
                     /label=Streptococcus pyogenes Cas9
                     /note="With a D to E mutation at AA 24"
     misc_feature    4355..4374
                     /label=O272_Cas9_seq2_F
                     /note="O272_Cas9_seq2_F"
     misc_feature    complement(4540..4559)
                     /label=O271_Cas9_seq1_R
                     /note="O271_Cas9_seq1_R"
     misc_feature    5741..5761
                     /label=O274_Cas9_seq4_F
                     /note="O274_Cas9_seq4_F"
     misc_feature    complement(5944..5965)
                     /label=O273_Cas9_seq3_R
                     /note="O273_Cas9_seq3_R"
     misc_feature    7210..7257
                     /label=nls
                     /note="nls"
     primer_bind     complement(7237..7277)
                     /label=O270_His-Cas9_R
     misc_feature    7266..7911
                     /label=His2Av
                     /note="His2Av stop, including 3'UTR and polyA signal"
     3'UTR           7270..7592
                     /label=3'UTR of His2Av
                     /note="3'UTR of His2Av"
     misc_feature    7499..7519
                     /label=3'UTR screening primer
                     /note="3'UTR screening primer"
     misc_feature    7556..7561
                     /label=polyA
                     /note="polyA signal predicted by Softberry-FGENESH"
     misc_feature    7917..8303
                     /label=in
                     /note="gypsy insulator"
     promoter        8304..8782
                     /label=Pwhite
                     /note="white mini-promoter and 5'UTR"
     gene            8783..11653
                     /label=mini-white
     misc_feature    12433..13017
                     /label=P5'
                     /note="P element 5' end"
     misc_feature    13018..13245
                     /label=linker
                     /note="white linker sequences"
     misc_feature    13246..15256
                     /label=pUC8
                     /note="pUC8 plasmid DNA"
     misc_recomb     15257..15539
                     /label=attB
     misc_feature    15559..16062
                     /label=linker
                     /note="white linker sequences"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttggctgcat cacgtaataa gtgtgcgttg aatttattcg
      661 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
      721 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
      781 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
      841 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
      901 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
      961 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     1021 tcggcaacag catgctgcag aagacttatc acaagtttgt acaaaaaagc tgaacgagaa
     1081 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     1141 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
     1201 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     1261 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     1321 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     1381 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1441 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1501 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1561 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1621 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1681 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     1741 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1801 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1861 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1921 atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt
     1981 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     2041 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     2101 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     2161 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     2221 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     2281 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     2341 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     2401 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
     2461 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     2521 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     2581 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     2641 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     2701 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgattt
     2761 gtcaaggtac cgagcgccgg agtataaata gaggcgcttc gtctacggag ctcagttgtg
     2821 aatgaatgtt cgagccgagc agacgtgccg cgcgaaagct aagcaaataa acaagcgcag
     2881 ctgagcaagc taaacaatcg taaagtgcaa gttaaagtga atcaattaaa agtaaccagc
     2941 aaccaagtaa atcaactgca actactgaaa tctgccggtg agattgtaca cccttcttaa
     3001 agatcccctt caaagtctaa attcttcctt ttcgtttagc caagacaaat caaaatggcc
     3061 ccaaagaaga agcggaaggt cggtatccac ggtgtcccag cagccatgga caagaagtac
     3121 tccattgggc tcgatatcgg cacaaacagc gtcggctggg ccgtcattac ggacgagtac
     3181 aaggtgccga gcaaaaaatt caaagttctg ggcaataccg atcgccacag cataaagaag
     3241 aacctcattg gcgccctcct gttcgactcc ggggagacgg ccgaagccac gcggctcaaa
     3301 agaacagcac ggcgcagata tacccgcaga aagaatcgga tctgctacct gcaggagatc
     3361 tttagtaatg agatggctaa ggtggatgac tctttcttcc ataggctgga ggagtccttt
     3421 ttggtggagg aggataaaaa gcacgagcgc cacccaatct ttggcaatat cgtggacgag
     3481 gtggcgtacc atgaaaagta cccaaccata tatcatctga ggaagaagct tgtagacagt
     3541 actgataagg ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat caaatttcgg
     3601 ggacacttcc tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga caaactcttt
     3661 atccaactgg ttcagactta caatcagctt ttcgaagaga acccgatcaa cgcatccgga
     3721 gttgacgcca aagcaatcct gagcgctagg ctgtccaaat cccggcggct cgaaaacctc
     3781 atcgcacagc tccctgggga gaagaagaac ggcctgtttg gtaatcttat cgccctgtca
     3841 ctcgggctga cccccaactt taaatctaac ttcgacctgg ccgaagatgc caagcttcaa
     3901 ctgagcaaag acacctacga tgatgatctc gacaatctgc tggcccagat cggcgaccag
     3961 tacgcagacc tttttttggc ggcaaagaac ctgtcagacg ccattctgct gagtgatatt
     4021 ctgcgagtga acacggagat caccaaagct ccgctgagcg ctagtatgat caagcgctat
     4081 gatgagcacc accaagactt gactttgctg aaggcccttg tcagacagca actgcctgag
     4141 aagtacaagg aaattttctt cgatcagtct aaaaatggct acgccggata cattgacggc
     4201 ggagcaagcc aggaggaatt ttacaaattt attaagccca tcttggaaaa aatggacggc
     4261 accgaggagc tgctggtaaa gcttaacaga gaagatctgt tgcgcaaaca gcgcactttc
     4321 gacaatggaa gcatccccca ccagattcac ctgggcgaac tgcacgctat actcaggcgg
     4381 caagaggatt tctacccctt tttgaaagat aacagggaaa agattgagaa aatcctcaca
     4441 tttcggatac cctactatgt aggccccctc gcccggggaa attccagatt cgcgtggatg
     4501 actcgcaaat cagaagagac catcactccc tggaacttcg aggaagtcgt ggataagggg
     4561 gcctctgccc agtccttcat cgaaaggatg actaactttg ataaaaatct gcctaacgaa
     4621 aaggtgcttc ctaaacactc tctgctgtac gagtacttca cagtttataa cgagctcacc
     4681 aaggtcaaat acgtcacaga agggatgaga aagccagcat tcctgtctgg agagcagaag
     4741 aaagctatcg tggacctcct cttcaagacg aaccggaaag ttaccgtgaa acagctcaaa
     4801 gaagactatt tcaaaaagat tgaatgtttc gactctgttg aaatcagcgg agtggaggat
     4861 cgcttcaacg catccctggg aacgtatcac gatctcctga aaatcattaa agacaaggac
     4921 ttcctggaca atgaggagaa cgaggacatt cttgaggaca ttgtcctcac ccttacgttg
     4981 tttgaagata gggagatgat tgaagaacgc ttgaaaactt acgctcatct cttcgacgac
     5041 aaagtcatga aacagctcaa gaggcgccga tatacaggat gggggcggct gtcaagaaaa
     5101 ctgatcaatg ggatccgaga caagcagagt ggaaagacaa tcctggattt tcttaagtcc
     5161 gatggatttg ccaaccggaa cttcatgcag ttgatccatg atgactctct cacctttaag
     5221 gaggacatcc agaaagcaca agtttctggc cagggggaca gtcttcacga gcacatcgct
     5281 aatcttgcag gtagcccagc tatcaaaaag ggaatactgc agaccgttaa ggtcgtggat
     5341 gaactcgtca aagtaatggg aaggcataag cccgagaata tcgttatcga gatggcccga
     5401 gagaaccaaa ctacccagaa gggacagaag aacagtaggg aaaggatgaa gaggattgaa
     5461 gagggtataa aagaactggg gtcccaaatc cttaaggaac acccagttga aaacacccag
     5521 cttcagaatg agaagctcta cctgtactac ctgcagaacg gcagggacat gtacgtggat
     5581 caggaactgg acatcaatcg gctctccgac tacgacgtgg atcatatcgt gccccagtct
     5641 tttctcaaag atgattctat tgataataaa gtgttgacaa gatccgataa aaatagaggg
     5701 aagagtgata acgtcccctc agaagaagtt gtcaagaaaa tgaaaaatta ttggcggcag
     5761 ctgctgaacg ccaaactgat cacacaacgg aagttcgata atctgactaa ggctgaacga
     5821 ggtggcctgt ctgagttgga taaagcaggc ttcatcaaaa ggcagcttgt tgagacacgc
     5881 cagatcacca agcacgtggc ccaaattctc gattcacgca tgaacaccaa gtacgatgaa
     5941 aatgacaaac tgattcgaga ggtgaaagtt attactctga agtctaagct ggtctcagat
     6001 ttcagaaagg actttcagtt ttataaggtg agagagatca acaattacca ccatgcgcat
     6061 gatgcctacc tgaatgcagt ggtaggcact gcacttatca aaaaatatcc caagcttgaa
     6121 tctgaatttg tttacggaga ctataaagtg tacgatgtta ggaaaatgat cgcaaagtct
     6181 gagcaggaaa taggcaaggc caccgctaag tacttctttt acagcaatat tatgaatttt
     6241 ttcaagaccg agattacact ggccaatgga gagattcgga agcgaccact tatcgaaaca
     6301 aacggagaaa caggagaaat cgtgtgggac aagggtaggg atttcgcgac agtccggaag
     6361 gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg aagtacagac cggaggcttc
     6421 tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc tgatcgcacg caaaaaagat
     6481 tgggacccca agaaatacgg cggattcgat tctcctacag tcgcttacag tgtactggtt
     6541 gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa gcgtcaagga actgctgggc
     6601 atcacaatca tggagcgatc aagcttcgaa aaaaacccca tcgactttct cgaggcgaaa
     6661 ggatataaag aggtcaaaaa agacctcatc attaagcttc ccaagtactc tctctttgag
     6721 cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg agctgcagaa aggtaacgag
     6781 ctggcactgc cctctaaata cgttaatttc ttgtatctgg ccagccacta tgaaaagctc
     6841 aaagggtctc ccgaagataa tgagcagaag cagctgttcg tggaacaaca caaacactac
     6901 cttgatgaga tcatcgagca aataagcgaa ttctccaaaa gagtgatcct cgccgacgct
     6961 aacctcgata aggtgctttc tgcttacaat aagcacaggg ataagcccat cagggagcag
     7021 gcagaaaaca ttatccactt gtttactctg accaacttgg gcgcgcctgc agccttcaag
     7081 tacttcgaca ccaccataga cagaaagcgg tacacctcta caaaggaggt cctggacgcc
     7141 acactgattc atcagtcaat tacggggctc tatgaaacaa gaatcgacct ctctcagctc
     7201 ggtggagaca agcgtcctgc tgctactaag aaagctggtc aagctaagaa aaagaaataa
     7261 attaactaag ccagtcggca atcggacgcc ttcgaaacat gcaacactaa tgtttaattc
     7321 agatttcagc agagacaagc taaaacaccg acgagttgta atcatttctg tgcgccagca
     7381 tatatttctt atatacaacg taatacataa ttatgtaatt ctagcatctc cccaacactc
     7441 acatacaaac aaacaaaaaa tacaaacaca caaaacgtat ttacccgcac gcatccttgg
     7501 cgaggttgag tatgaaacaa aaacaaaact taatttagag caaagtaatt acacgaataa
     7561 atttaataaa aaaaaactat aataaaaacg ccaatcatgt tatttccaaa aaaaaaaaaa
     7621 aaaaaaaaac tagacgagat gtttgcgctt gtgtcgatga atgtaaagaa ttgcaactac
     7681 atagatggta attaatttca gctgtaagaa gtacaagttt ttcataattg tgagaaaagt
     7741 gcagtagtat cgcttaaagt tcagttttaa tcagggcatt tagcagctcg aacccaatgt
     7801 atttgtaagc ccaataaacg tgtaaatgcc tagagcaaaa tatcacaact gtagcttttc
     7861 atccgaaaga taataatgaa tagactgacc tgacgttttt caaaatgcgt aactagtcac
     7921 gtaataagtg tgcgttgaat ttattcgcaa aaacattgca tattttcggc aaagtaaaat
     7981 tttgttgcat accttatcaa aaaataagtg ctgcatactt tttagagaaa ccaaataatt
     8041 ttttattgca tacccgtttt taataaaata cattgcatac cctcttttaa taaaaaatat
     8101 tgcatacttt gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat
     8161 acccgttttt aataaaatac attgcatacc ctcttttaat aaaaaatatt gcatacgttg
     8221 acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata ccttttcttg
     8281 ccataccatt tagccgatca attctagtat gtatgtaagt taataaaacc catttttgcg
     8341 gaaagtagat aaaaaaaaca tttttttttt ttactgcact ggatatcatt gaacttatct
     8401 gatcagtttt aaatttactt cgatccaagg gtatttgatg taccaggttc tttcgattac
     8461 ctctcactca aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac
     8521 agaaatatcg ccgtctcttt cgccgctgcg tccgctatct ctttcgccac cgtttgtagc
     8581 gttacgtagc gtcaatgtcc gccttcagtt gcattttgtc agcggtttcg tgacgaagct
     8641 ccaagcggtt tacgccatca attaaacaca aagtgctgtg ccaaaactcc tctcgcttct
     8701 tatttttgtt tgttttttga gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg
     8761 gaaagtgtga aaaatcccgg caatgggcca agaggatcag gagctattaa ttcgcggagg
     8821 cagcaaacac ccatctgccg agcatctgaa caatgtgagt agtacatgtg catacatctt
     8881 aagttcactt gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac
     8941 tgcgacccac aaaaatccca aaccgcaatc gcacaaacaa atagtgacac gaaacagatt
     9001 attctggtag ctgttctcgc tatataagac aatttttgag atcatatcat gatcaagaca
     9061 tctaaaggca ttcattttcg actatattct tttttacaaa aaatataaca accagatatt
     9121 ttaagctgat cctagatgca caaaaaataa ataaaagtat aaacctactt cgtaggatac
     9181 ttcggggtac tttttgttcg gggttagatg agcataacgc ttgtagttga tatttgagat
     9241 cccctatcat tgcagggtga cagcggacgc ttcgcagagc tgcattaacc agggcttcgg
     9301 gcaggccaaa aactacggca cgctccggcc acccagtccg ccggaggact ccggttcagg
     9361 gagcggccaa ctagccgaga acctcaccta tgcctggcac aatatggaca tctttggggc
     9421 ggtcaatcag ccgggtcccg gatggcggca gctggtcaac cggacacgcg gactattctg
     9481 caacgagcga cacataccgg cgcccaggaa acatttgctc aagaacggtg agtttctatt
     9541 cgcagtcggc tgatctgtgt gaaatcttaa taaagggtcc aattaccaat ttgaaactca
     9601 gtttgcggcg tggcctatcc gggcgaactt ttggccgtga tgggcagttc cggtgccgga
     9661 aagacgaccc tgctgaatgc ccttgccttt cgatcgccgc agggcatcca agtatcgcca
     9721 tccgggatgc gactgctcaa tggccaacct gtggacgcca aggagatgca ggccaggtgc
     9781 gcctatgtcc agcaggatga cctctttatc ggctccctaa cggccaggga acacctgatt
     9841 ttccaagcca tggtgcggat gccacgacat ctgacctatc ggcagcgagt ggcccgcgtg
     9901 gatcaggtga tccaggagct ttcgctcagc aaatgtcagc acacgatcat cggtgtgccc
     9961 ggcagggtga aaggtctgtc cggcggagaa aggaagcgtc tggcattcgc ctccgaggct
    10021 ctaaccgatc cgccgcttct gatctgcgat gagcccacct ccggactgga ctcctttacc
    10081 gcccacagcg tcgtccaggt gctgaagaag ctgtcgcaga agggcaagac cgtcatcctg
    10141 accattcatc agccgtcttc cgagctgttt gagctctttg acaagatcct tctgatggcc
    10201 gagggcaggg tagctttctt gggcactccc agcgaagccg tcgacttctt ttcctagtga
    10261 gttcgatgtg tttattaagg gtatctagta ttacataaca tctcaactcc tatccagcgt
    10321 gggtgcccag tgtcctacca actacaatcc ggcggacttt tacgtacagg tgttggccgt
    10381 tgtgcccgga cgggagatcg agtcccgtga tcggatcgcc aagatatgcg acaattttgc
    10441 cattagcaaa gtagcccggg atatggagca gttgttggcc accaaaaatc tggagaagcc
    10501 actggagcag ccggagaatg ggtacaccta caaggccacc tggttcatgc agttccgggc
    10561 ggtcctgtgg cgatcctggc tgtcggtgct caaggaacca ctcctcgtaa aagtgcgact
    10621 tattcagaca acggtgagtg gttccagtgg aaacaaatga tataacgctt acaattcttg
    10681 gaaacaaatt cgctagattt tagatagaat tgcctgattc cacacccttc ttagtttttt
    10741 tcaatgagat gtatagttta tagttttgca gaagataaat aaatttcatt taactcgcga
    10801 atattaatga gatgcgagta acattttaat ttgcagatgg ttgccatctt gattggcctc
    10861 atctttttgg gccaacaact cacgcaagtg ggtgtgatga atatcaacgg agccatcttc
    10921 ctcttcctga ccaacatgac ctttcaaaac gtctttgcca cgataaatgt aagtcatgtt
    10981 tagaatacat ttgcatttca ataatttact aactttctaa tgaatcgatt cgatttaggt
    11041 gttcacctca gagctgccag tttttatgag ggaggcccga agtcgacttt atcgctgtga
    11101 cacatacttt ctgggcaaaa cgattgccga attgccgctt tttctcacag tgccactggt
    11161 cttcacggcg attgcctatc cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa
    11221 ctgcctggcg ctggtcactc tggtggccaa tgtgtcaacg tccttcggat atctaatatc
    11281 ctgcgccagc tcctcgacct cgatggcgct gtctgtgggt ccgccggtta tcataccatt
    11341 cctgctcttt ggcggcttct tcttgaactc gggctcggtg ccagtatacc tcaaatggtt
    11401 gtcgtacctc tcatggttcc gttacgccaa cgagggtctg ctgattaacc aatgggcgga
    11461 cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa
    11521 ggtcatcctg gagacgctta acttctccgc cgccgatctg ccgctggact acgtgggtct
    11581 ggccattctc atcgtgagct tccgggtgct cgcatatctg gctctaagac ttcgggcccg
    11641 acgcaaggag tagccgacat atatccgaaa taactgcttg tttttttttt ttaccattat
    11701 taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca
    11761 ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt
    11821 agactttgga tttgtcttct aaccaaaaga cttacacacc tgcatacctt acatcaaaaa
    11881 ctcgtttatc gctacataaa acaccgggat atatttttta tatacatact tttcaaatcg
    11941 cgcgccctct tcataattca cctccaccac accacgtttc gtagttgctc tttcgctgtc
    12001 tcccacccgc tctccgcaac acattcacct tttgttcgac gaccttggag cgactgtcgt
    12061 tagttccgcg cgattcggtt cgctcaaatg gttccgagtg gttcatttcg tctcaataga
    12121 aattagtaat aaatatttgt atgtacaatt tatttgctcc aatatatttg tatatatttc
    12181 cctcacagct atatttattc taatttaata ttatgacttt ttaaggtaat tttttgtgac
    12241 ctgttcggag tgattagcgt tacaatttga actgaaagtg acatccagtg tttgttcctt
    12301 gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata tatcaaaaat
    12361 aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc actggaacta
    12421 gaattaattc ggctgctgct ctaaacgacg catttcgtac tccaaagtac gaattttttc
    12481 cctcaagctc ttattttcat taaacaatga acaggaccta acgcacagtc acgttattgt
    12541 ttacataaat gatttttttt actattcaaa cttactctgt ttgtgtactc ccactggtat
    12601 agccttcttt tatcttttct ggttcaggct ctatcacttt actaggtacg gcatctgcgt
    12661 tgagtcgcct ccttttaaat gtctgacctt ttgcaggtgc agccttccac tgcgaatcat
    12721 taaagtgggt atcacaaatt tgggagtttt caccaaggct gcacccaagg ctctgctccc
    12781 acaattttct cttaatagca cacttcggca cgtgaattaa ttttactcca gtcacagctt
    12841 tgcagcaaaa tttgcaatat ttcatttttt tttattccac gtaagggtta atgttttcaa
    12901 aaaaaaattc gtccgcacac aacctttcct ctcaacaagc aaacgtgcac tgaatttaag
    12961 tgtatacttc ggtaagcttc ggctatcgac gggaccacct tatgttattt catcatgggc
    13021 cagacccacg tagtccagcg gcagatcggc ggcggagaag ttaagcgtct ccaggatgac
    13081 cttgcccgaa ctggggcacg tggtgttcga cgatgtgcag ctaatttcgc ccggctccac
    13141 gtccgcccat tggttaatca gcagaccctc gttggcgtaa cggaaccatg agaggtacga
    13201 caaccatttg aggtatactg gcaccgagcc cgagttcaag aagaaggcgt ttttccatag
    13261 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    13321 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    13381 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    13441 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    13501 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    13561 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    13621 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    13681 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    13741 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    13801 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    13861 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    13921 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    13981 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    14041 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    14101 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    14161 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    14221 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    14281 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    14341 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    14401 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    14461 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    14521 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    14581 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
    14641 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    14701 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    14761 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    14821 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    14881 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    14941 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    15001 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    15061 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    15121 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    15181 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
    15241 actgagagtg caccattcga catgcccgcc gtgaccgtcg agaacccgct gacgctgccc
    15301 cgcgtatccg cacccgccga cgccgtcgca cgtcccgtgc tcaccgtgac caccgcgccc
    15361 agcggtttcg agggcgaggg cttcccggtg cgccgcgcgt tcgccgggat caactaccgc
    15421 cacctcgacc cgttcatcat gatggaccag atgggtgagg tggagtacgc gcccggggag
    15481 cccaagggca cgccctggca cccgcaccgc ggcttcgaga ccgtgaccta catcgtcgat
    15541 atgcggtgtg aaataccgca ccgaatcgcg cggaactaac gacagtcgct ccaaggtcgt
    15601 cgaacaaaag gtgaatgtgt tgcggagagc gggtgggaga cagcgaaaga gcaactacga
    15661 aacgtggtgt ggtggaggtg aattatgaag agggcgcgcg atttgaaaag tatgtatata
    15721 aaaaatatat cccggtgttt tatgtagcga taaacgagtt tttgatgtaa ggtatgcagg
    15781 tgtgtaagtc ttttggttag aagacaaatc caaagtctac ttgtggggat gttcgaaggg
    15841 gaaatacttg tattctatag gtcatatctt gtttttattg gcacaaatat aattacatta
    15901 gctttttgag ggggcaataa acagtaaaca cgatggtaat aatggtaaaa aaaaaaaaca
    15961 agcagttatt tcggatatat gtcggctact ccttgcgtcg ggcccgaagt cttagagcca
    16021 gatatgcgag cacccggaag ctcacgatga gaatggccag ac
//
