LOCUS       Exported               14512 bp DNA     circular SYN 04-JUN-2021
DEFINITION  A Gateway destination vector for expressing enhancer-driven Gal4 in 
            Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    pDEST-APIGH
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14512)
  AUTHORS   Han C, Wang D, Soba P, Zhu S, Lin X, Jan LY, Jan YN.
  TITLE     Integrins regulate repulsion-mediated dendritic patterning of 
            drosophila sensory neurons by restricting dendrites in a 2D space.
  JOURNAL   Neuron 2012;73:64-78.
  PUBMED    22243747
REFERENCE   2  (bases 1 to 14512)
  AUTHORS   Chun Han
  TITLE     Direct Submission
  JOURNAL   Exported Jun 26, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron"; 
            date: "2012"; volume: "73"; pages: "64-78"
FEATURES             Location/Qualifiers
     source          1..14512
                     /organism="synthetic DNA construct"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     misc_feature    240..631
                     /label=Stuffing
                     /note="Stuffing sequencing from neo gene coding region"
     protein_bind    632..1017
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     1014..1032
                     /label=O7_PelicanH_5F
     misc_feature    1048..2758
                     /label=Gateway RfA cassette
                     /note="Contains attR1 --> attR2"
     protein_bind    1051..1175
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1200..1230
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(1210..1232)
                     /label=ccdB_casR
                     /note="ccdB_casR"
     CDS             1284..1943
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1352..1373)
                     /label=ccdB_casRII
     CDS             2285..2590
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2405..2426
                     /label=ccdB_casFII
     primer_bind     2491..2513
                     /label=ccdB_casF
                     /note="ccdB_casF"
     protein_bind    complement(2631..2755)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     TATA_signal     2772..2974
                     /label=Hsp70
                     /note="TATA box and 5'UTR from Hsp70Bb"
     primer_bind     complement(2824..2844)
                     /label=O6_HSP70TATA_R
     intron          2978..3039
                     /gene="zeste"
                     /label=zIn
                     /note="2nd intron of Drosophila zeste gene"
     CDS             3055..5700
                     /gene="Gal4"
                     /label=Gal4
     misc_feature    5715..6360
                     /label=His2Av stop
                     /note="His2Av stop, including 3'UTR and polyA signal"
     misc_feature    5948..5968
                     /label=3'UTR screening primer
                     /note="3'UTR screening primer"
     misc_feature    6005..6010
                     /label=polyA
                     /note="polyA signal predicted by Softberry-FGENESH"
     protein_bind    6367..6752
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     CDS             join(7232..7303,7705..7978,8053..8707,8769..9084,9288..9419,
                     9490..10104)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    10883..11468
                     /label=P element 5' end
     misc_feature    11469..11696
                     /label=linker
                     /note="white linker sequences"
     rep_origin      complement(11703..12291)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12462..13322)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13323..13427)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    13901..13970
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    14010..14512
                     /label=linker
                     /note="white linker sequences"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttgggctgca tcacgtaata agtgtgcgtt gaatttattc
      661 gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta tcaaaaaata
      721 agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg tttttaataa
      781 aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa acaaattttc
      841 gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa atacattgca
      901 taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg ttgcataccc
      961 aataaaagat tattatattg catacctttt cttgccatac catttagccg atcaattgtg
     1021 ctcggcaaca gcatgctgca gcagatcatc acaagtttgt acaaaaaagc tgaacgagaa
     1081 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     1141 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
     1201 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     1261 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     1321 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     1381 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1441 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1501 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1561 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1621 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1681 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     1741 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1801 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1861 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1921 atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt
     1981 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     2041 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     2101 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     2161 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     2221 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     2281 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     2341 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     2401 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
     2461 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     2521 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     2581 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     2641 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     2701 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgatga
     2761 tctggtctag cgagcgccgg agtataaata gaggcgcttc gtctacggag cgacaattca
     2821 attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag
     2881 ctgagcaagc taaacaatcg taaagtgcaa gttaaagtga atcaattaaa agtaaccagc
     2941 aaccaagtaa atcaactgca actactgaaa tctgccggtg agattgtaca cccttcttaa
     3001 agatcccctt caaagtctaa attcttcctt ttcgtttagc caagactcga caaaatgaag
     3061 ctactgtctt ctatcgaaca agcatgcgat atttgccgac ttaaaaagct caagtgctcc
     3121 aaagaaaaac cgaagtgcgc caagtgtctg aagaacaact gggagtgtcg ctactctccc
     3181 aaaaccaaaa ggtctccgct gactagggca catctgacag aagtggaatc aaggctagaa
     3241 agactggaac agctatttct actgattttt cctcgagaag accttgacat gattttgaaa
     3301 atggattctt tacaggatat aaaagcattg ttaacaggat tatttgtaca agataatgtg
     3361 aataaagatg ccgtcacaga tagattggct tcagtggaga ctgatatgcc tctaacattg
     3421 agacagcata gaataagtgc gacatcatca tcggaagaga gtagtaacaa aggtcaaaga
     3481 cagttgactg tatcgattga ctcggcagct catcatgata actccacaat tccgttggat
     3541 tttatgccca gggatgctct tcatggattt gattggtctg aagaggatga catgtcggat
     3601 ggcttgccct tcctgaaaac ggaccccaac aataatgggt tctttggcga cggttctctc
     3661 ttatgtattc ttcgatctat tggctttaaa ccggaaaatt acacgaactc taacgttaac
     3721 aggctcccga ccatgattac ggatagatac acgttggctt ctagatccac aacatcccgt
     3781 ttacttcaaa gttatctcaa taattttcac ccctactgcc ctatcgtgca ctcaccgacg
     3841 ctaatgatgt tgtataataa ccagattgaa atcgcgtcga aggatcaatg gcaaatcctt
     3901 tttaactgca tattagccat tggagcctgg tgtatagagg gggaatctac tgatatagat
     3961 gttttttact atcaaaatgc taaatctcat ttgacgagca aggtcttcga gtcaggttcc
     4021 ataattttgg tgacagccct acatcttctg tcgcgatata cacagtggag gcagaaaaca
     4081 aatactagct ataattttca cagcttttcc ataagaatgg ccatatcatt gggcttgaat
     4141 agggacctcc cctcgtcctt cagtgatagc agcattctgg aacaaagacg ccgaatttgg
     4201 tggtctgtct actcttggga gatccaattg tccctgcttt atggtcgatc catccagctt
     4261 tctcagaata caatctcctt cccttcttct gtggacgatg tgcagcgtac cacaacaggt
     4321 cccaccatat atcatggcat cattgaaaca gcaaggctct tacaagtttt cacaaaaatc
     4381 tatgaactag acaaaacagt aactgcagaa aaaagtccta tatgtgcaaa aaaatgcttg
     4441 atgatttgta atgagattga ggaggtttcg agacaggcac caaagttttt acaaatggat
     4501 atttccacca ccgctctaac caatttgttg aaggaacacc cttggctatc ctttacaaga
     4561 ttcgaactga agtggaaaca gttgtctctt atcatttatg tattaagaga ttttttcact
     4621 aattttaccc agaaaaagtc acaactagaa caggatcaaa atgatcatca aagttatgaa
     4681 gttaaacgat gctccatcat gttaagcgat gcagcacaaa gaactgttat gtctgtaagt
     4741 agctatatgg acaatcataa tgtcacccca tattttgcct ggaattgttc ttattacttg
     4801 ttcaatgcag tcctagtacc cataaagact ctactctcaa actcaaaatc gaatgctgag
     4861 aataacgaga ccgcacaatt attacaacaa attaacactg ttctgatgct attaaaaaaa
     4921 ctggccactt ttaaaatcca gacttgtgaa aaatacattc aagtactgga agaggtatgt
     4981 gcgccgtttc tgttatcaca gtgtgcaatc ccattaccgc atatcagtta taacaatagt
     5041 aatggtagcg ccattaaaaa tattgtcggt tctgcaacta tcgcccaata ccctactctt
     5101 ccggaggaaa atgtcaacaa tatcagtgtt aaatatgttt ctcctggctc agtagggcct
     5161 tcacctgtgc cattgaaatc aggagcaagt ttcagtgatc tagtcaagct gttatctaac
     5221 cgtccaccct ctcgtaactc tccagtgaca ataccaagaa gcacaccttc gcatcgctca
     5281 gtcacgcctt ttctagggca acagcaacag ctgcaatcat tagtgccact gaccccgtct
     5341 gctttgtttg gtggcgccaa ttttaatcaa agtgggaata ttgctgatag ctcattgtcc
     5401 ttcactttca ctaacagtag caacggtccg aacctcataa caactcaaac aaattctcaa
     5461 gcgctttcac aaccaattgc ctcctctaac gttcatgata acttcatgaa taatgaaatc
     5521 acggctagta aaattgatga tggtaataat tcaaaaccac tgtcacctgg ttggacggac
     5581 caaactgcgt ataacgcgtt tggaatcact acagggatgt ttaataccac tacaatggat
     5641 gatgtatata actatctatt cgatgatgaa gataccccac caaacccaaa aaaagagtaa
     5701 ctagaattaa ttaactaagc cagtcggcaa tcggacgcct tcgaaacatg caacactaat
     5761 gtttaattca gatttcagca gagacaagct aaaacaccga cgagttgtaa tcatttctgt
     5821 gcgccagcat atatttctta tatacaacgt aatacataat tatgtaattc tagcatctcc
     5881 ccaacactca catacaaaca aacaaaaaat acaaacacac aaaacgtatt tacccgcacg
     5941 catccttggc gaggttgagt atgaaacaaa aacaaaactt aatttagagc aaagtaatta
     6001 cacgaataaa tttaataaaa aaaaactata ataaaaacgc caatcatgtt atttccaaaa
     6061 aaaaaaaaaa aaaaaaaact agacgagatg tttgcgcttg tgtcgatgaa tgtaaagaat
     6121 tgcaactaca tagatggtaa ttaatttcag ctgtaagaag tacaagtttt tcataattgt
     6181 gagaaaagtg cagtagtatc gcttaaagtt cagttttaat cagggcattt agcagctcga
     6241 acccaatgta tttgtaagcc caataaacgt gtaaatgcct agagcaaaat atcacaactg
     6301 tagcttttca tccgaaagat aataatgaat agactgacct gacgtttttc aaaatgcgta
     6361 actagtcacg taataagtgt gcgttgaatt tattcgcaaa aacattgcat attttcggca
     6421 aagtaaaatt ttgttgcata ccttatcaaa aaataagtgc tgcatacttt ttagagaaac
     6481 caaataattt tttattgcat acccgttttt aataaaatac attgcatacc ctcttttaat
     6541 aaaaaatatt gcatactttg acgaaacaaa ttttcgttgc atacccaata aaagattatt
     6601 atattgcata cccgttttta ataaaataca ttgcataccc tcttttaata aaaaatattg
     6661 catacgttga cgaaacaaat tttcgttgca tacccaataa aagattatta tattgcatac
     6721 cttttcttgc cataccattt agccgatcaa ttctagtatg tatgtaagtt aataaaaccc
     6781 atttttgcgg aaagtagata aaaaaaacat tttttttttt tactgcactg gatatcattg
     6841 aacttatctg atcagtttta aatttacttc gatccaaggg tatttgatgt accaggttct
     6901 ttcgattacc tctcactcaa aatgacattc cactcaaagt cagcgctgtt tgcctccttc
     6961 tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc tttcgccacc
     7021 gtttgtagcg ttacgtagcg tcaatgtccg ccttcagttg cattttgtca gcggtttcgt
     7081 gacgaagctc caagcggttt acgccatcaa ttaaacacaa agtgctgtgc caaaactcct
     7141 ctcgcttctt atttttgttt gttttttgag tgattggggt ggtgattggt tttgggtggg
     7201 taagcagggg aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg agctattaat
     7261 tcgcggaggc agcaaacacc catctgccga gcatctgaac aatgtgagta gtacatgtgc
     7321 atacatctta agttcacttg atctatagga actgcgattg caacatcaaa ttgtctgcgg
     7381 cgtgagaact gcgacccaca aaaatcccaa accgcaatcg cacaaacaaa tagtgacacg
     7441 aaacagatta ttctggtagc tgttctcgct atataagaca atttttgaga tcatatcatg
     7501 atcaagacat ctaaaggcat tcattttcga ctatattctt ttttacaaaa aatataacaa
     7561 ccagatattt taagctgatc ctagatgcac aaaaaataaa taaaagtata aacctacttc
     7621 gtaggatact tcggggtact ttttgttcgg ggttagatga gcataacgct tgtagttgat
     7681 atttgagatc ccctatcatt gcagggtgac agcggagcgg cttcgcagag ctgcattaac
     7741 cagggcttcg ggcaggccaa aaactacggc acgctccggc cacccagtcc gccggaggac
     7801 tccggttcag ggagcggcca actagccgag aacctcacct atgcctggca caatatggac
     7861 atctttgggg cggtcaatca gccgggctcc ggatggcggc agctggtcaa ccggacacgc
     7921 ggactattct gcaacgagcg acacataccg gcgcccagga aacatttgct caagaacggt
     7981 gagtttctat tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc caattaccaa
     8041 tttgaaactc agtttgcggc gtggcctatc cgggcgaact tttggccgtg atgggcagtt
     8101 ccggtgccgg aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg cagggcatcc
     8161 aagtatcgcc atccgggatg cgactgctca atggccaacc tgtggacgcc aaggagatgc
     8221 aggccaggtg cgcctatgtc cagcaggatg acctctttat cggctcccta acggccaggg
     8281 aacacctgat tttccaagcc atggtgcgga tgccacgaca tctgacctat cggcagcgag
     8341 tggcccgcgt ggatcaggtg atccaggagc tttcgctcag caaatgtcag cacacgatca
     8401 tcggtgtgcc cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt ctggcattcg
     8461 cctccgaggc tctaaccgat ccgccgcttc tgatctgcga tgagcccacc tccggactgg
     8521 actcctttac cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag aagggcaaga
     8581 ccgtcatcct gaccattcat cagccgtctt ccgagctgtt tgagctcttt gacaagatcc
     8641 ttctgatggc cgagggcagg gtagctttct tgggcactcc cagcgaagcc gtcgacttct
     8701 tttcctagtg agttcgatgt gtttattaag ggtatctagt attacataac atctcaactc
     8761 ctatccagcg tgggtgccca gtgtcctacc aactacaatc cggcggactt ttacgtacag
     8821 gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc caagatatgc
     8881 gacaattttg ccattagcaa agtagcccgg gatatggagc agttgttggc caccaaaaat
     8941 ctggagaagc cactggagca gccggagaat gggtacacct acaaggccac ctggttcatg
     9001 cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc actcctcgta
     9061 aaagtgcgac ttattcagac aacggtgagt ggttccagtg gaaacaaatg atataacgct
     9121 tacaattctt ggaaacaaat tcgctagatt ttagatagaa ttgcctgatt ccacaccctt
     9181 cttagttttt ttcaatgaga tgtatagttt atagttttgc agaagataaa taaatttcat
     9241 ttaactcgcg aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct
     9301 tgattggcct catctttttg ggccaacaac tcacgcaagt gggtgtgatg aatatcaacg
     9361 gagccatctt cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg
     9421 taagtcatgt ttagaataca tttgcatttc aataatttac taactttcta atgaatcgat
     9481 tcgatttagg tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt
     9541 tatcgctgtg acacatactt tctgggcaaa acgattgccg aattgccgct ttttctcaca
     9601 gtgccactgg tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg
     9661 cacttcttca actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga
     9721 tatctaatat cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt
     9781 atcataccat tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac
     9841 ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac
     9901 caatgggcgg acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc
     9961 agttcgggca aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac
    10021 tacgtgggtc tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga
    10081 cttcgggccc gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt
    10141 tttaccatta ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta
    10201 tatttgtgcc aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca
    10261 tccccacaag tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct
    10321 tacatcaaaa actcgtttat cgctacataa aacaccggga tatatttttt atatacatac
    10381 ttttcaaatc gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct
    10441 ctttcgctgt ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga
    10501 gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc
    10561 gtctcaatag aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt
    10621 gtatatattt ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa
    10681 ttttttgtga cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt
    10741 gtttgttcct tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat
    10801 atatcaaaaa taacaactat aataataaga atacatttaa tttagaaaat gcttggattt
    10861 cactggaact agaattaatt cggctgctgc tctaaacgac gcatttcgta ctccaaagta
    10921 cgaatttttt ccctcaagct cttattttca ttaaacaatg aacaggacct aacgcacagt
    10981 cacgttattg tttacataaa tgattttttt tactattcaa acttactctg tttgtgtact
    11041 cccactggta tagccttctt ttatcttttc tggttcaggc tctatcactt tactaggtac
    11101 ggcatctgcg ttgagtcgcc tccttttaaa tgtctgacct tttgcaggtg cagccttcca
    11161 ctgcgaatca ttaaagtggg tatcacaaat ttgggagttt tcaccaaggc tgcacccaag
    11221 gctctgctcc cacaattttc tcttaatagc acacttcggc acgtgaatta attttactcc
    11281 agtcacagct ttgcagcaaa atttgcaata tttcattttt ttttattcca cgtaagggtt
    11341 aatgttttca aaaaaaaatt cgtccgcaca caacctttcc tctcaacaag caaacgtgca
    11401 ctgaatttaa gtgtatactt cggtaagctt cggctatcga cgggaccacc ttatgttatt
    11461 tcatcatggg ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc
    11521 tccaggatga ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg
    11581 cccggctcca cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat
    11641 gagaggtacg acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaaggcg
    11701 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    11761 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    11821 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    11881 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    11941 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    12001 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    12061 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    12121 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    12181 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    12241 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    12301 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    12361 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    12421 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    12481 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    12541 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    12601 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    12661 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    12721 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    12781 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    12841 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    12901 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    12961 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    13021 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
    13081 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    13141 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    13201 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    13261 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    13321 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    13381 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    13441 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    13501 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
    13561 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
    13621 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
    13681 gagcagattg tactgagagt gcaccattcg acatgcccgc cgtgaccgtc gagaacccgc
    13741 tgacgctgcc ccgcgtatcc gcacccgccg acgccgtcgc acgtcccgtg ctcaccgtga
    13801 ccaccgcgcc cagcggtttc gagggcgagg gcttcccggt gcgccgcgcg ttcgccggga
    13861 tcaactaccg ccacctcgac ccgttcatca tgatggacca gatgggtgag gtggagtacg
    13921 cgcccgggga gcccaagggc acgccctggc acccgcaccg cggcttcgag accgtgacct
    13981 acatcgtcga tatgcggtgt gaaataccgc accgaatcgc gcggaactaa cgacagtcgc
    14041 tccaaggtcg tcgaacaaaa ggtgaatgtg ttgcggagag cgggtgggag acagcgaaag
    14101 agcaactacg aaacgtggtg tggtggaggt gaattatgaa gagggcgcgc gatttgaaaa
    14161 gtatgtatat aaaaaatata tcccggtgtt ttatgtagcg ataaacgagt ttttgatgta
    14221 aggtatgcag gtgtgtaagt cttttggtta gaagacaaat ccaaagtcta cttgtgggga
    14281 tgttcgaagg ggaaatactt gtattctata ggtcatatct tgtttttatt ggcacaaata
    14341 taattacatt agctttttga gggggcaata aacagtaaac acgatggtaa taatggtaaa
    14401 aaaaaaaaca agcagttatt tcggatatat gtcggctact ccttgcgtcg ggcccgaagt
    14461 cttagagcca gatatgcgag cacccggaag ctcacgatga gaatggccag ac
//
