LOCUS       pDEST-HemmarI2              14615 bp ds-DNA     circular SYN 02-OCT-2017
DEFINITION  Gateway destination vector for expressing enhancer-driven CD4-IFP2.0
            in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
FEATURES             Location/Qualifiers
     source          1..14615
                     /organism="synthetic DNA construct"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    631..1016
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     misc_feature    1048..2758
                     /label=Gateway RfA cassette
                     /note="Contains attR1 --> attR2"
     protein_bind    1051..1175
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1200..1230
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     misc_feature    complement(1210..1232)
                     /label=ccdB_casR
                     /note="ccdB_casR"
     CDS             1284..1943
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2285..2590
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2631..2755)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     TATA_signal     2772..2974
                     /label=Hsp70
                     /note="TATA box and 5'UTR from Hsp70Bb"
     promoter        2772..2974
                     /label=DSCPm
                     /note="DSCPm: modified Drosophila synthetic core promoter 
                     based on Hsp70"
     misc_feature    2783..2788
                     /label=TATA
                     /note="TATA box"
     misc_feature    2812..2817
                     /label=Inr
                     /note="Inr. A is the transcription starting site (TSS)"
     misc_feature    2831..2840
                     /label=MTE
                     /note="MTE"
     misc_feature    2841..2846
                     /label=DPE
                     /note="DPE"
     intron          2978..3039
                     /gene="zeste"
                     /label=zIn
                     /note="2nd intron of Drosophila zeste gene"
     misc_signal     3072..3092
                     /label=Syn21
                     /note="Syn21 translational enhancer"
     CDS             3093..5801
                     /label=CD4-IFP2.0-HO1
     sig_peptide     3093..3161
                     /label=Akh SP
                     /note="signal peptide of Drosophila Akh, cleaved after 
                     AA22. modified"
     misc_feature    3168..3197
                     /label=Linker
                     /note="Peptide linker"
     CDS             3198..3869
                     /label=CD4-2
                     /note="CD4-2 CDS"
     misc_feature    3870..3884
                     /label=GS linker
     CDS             3912..4874
                     /codon_start=1
                     /product="bacteriophytochrome-based monomeric infrared 
                     fluorescent protein (Yu et al., 2014)"
                     /label=IFP2.0
                     /translation="MARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
                     GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLD
                     WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
                     VRELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLR
                     LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
                     QLWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
     CDS             4878..4889
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4881..4934
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4935..5801
                     /label=HO1
     misc_feature    5817..6462
                     /label=His2Av stop
                     /note="His2Av stop, including 3'UTR and polyA signal"
     3'UTR           5821..6143
                     /label=3'UTR of His2Av
                     /note="3'UTR of His2Av"
     protein_bind    6469..6854
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     CDS             join(7334..7405,7807..8080,8155..8809,8871..9186,9390..9521,
                     9592..10206)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    10985..11570
                     /label=P element 5' end
     misc_feature    11571..11798
                     /label=linker
                     /note="white linker sequences"
     rep_origin      complement(11805..12393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12564..13424)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13425..13529)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    14003..14072
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    14112..14615
                     /label=linker
                     /note="white linker sequences"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttggctgcat cacgtaataa gtgtgcgttg aatttattcg
      661 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
      721 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
      781 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
      841 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
      901 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
      961 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     1021 tcggcaacag catgctgcag aagacttatc acaagtttgt acaaaaaagc tgaacgagaa
     1081 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     1141 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
     1201 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     1261 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     1321 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     1381 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1441 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1501 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1561 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1621 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1681 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     1741 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1801 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1861 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1921 atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt
     1981 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     2041 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     2101 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     2161 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     2221 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     2281 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     2341 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     2401 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
     2461 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     2521 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     2581 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     2641 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     2701 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgattt
     2761 gtcaaggtac cgagcgccgg agtataaata gaggcgcttc gtctacggag ctcagttgtg
     2821 aatgaatgtt cgagccgagc agacgtgccg cgcgaaagct aagcaaataa acaagcgcag
     2881 ctgagcaagc taaacaatcg taaagtgcaa gttaaagtga atcaattaaa agtaaccagc
     2941 aaccaagtaa atcaactgca actactgaaa tctgccggtg agattgtaca cccttcttaa
     3001 agatcccctt caaagtctaa attcttcctt ttcgtttagc cactcgagag aaaaccggta
     3061 cgcgtgaatt caacttaaaa aaaaaaatca aaatgaatcc caagagcgaa gtcctcattg
     3121 cagccgtgct gttcatgctg ctggcctgcg tccagtgtca agctagcggt ggcggcggaa
     3181 gtggaggtgg aggctcgttc cagaaggcct ccagcatagt ctataagaaa gagggggaac
     3241 aggtggagtt ctccttccca ctcgccttta cagttgaaaa gctgacgggc agtggcgagc
     3301 tgtggtggca ggcggagagg gcttcctcct ccaagtcttg gatcaccttt gacctgaaga
     3361 acaaggaagt gtctgtaaaa cgggttaccc aggaccctaa gctccagatg ggcaagaagc
     3421 tcccgctcca cctcaccctg ccccaggcct tgcctcagta tgctggctct ggaaacctca
     3481 ccctggccct tgaagcgaaa acaggaaagt tgcatcagga agtgaacctg gtggtgatga
     3541 gagccactca gctccagaaa aatttgacct gtgaggtgtg gggacccacc tcccctaagc
     3601 tgatgctgag cttgaaactg gagaacaagg aggcaaaggt ctcgaagcgg gagaaggcgg
     3661 tgtgggtgct gaaccctgag gcggggatgt ggcagtgtct gctgagtgac tcgggacagg
     3721 tcctgctgga atccaacatc aaggttctgc ccacatggtc caccccggtg cagccaatgg
     3781 ccctgattgt gctggggggc gtcgccggcc tcctgctttt cattgggcta ggcatcttct
     3841 tctgtgtcag gtgccggcac cgaaggcgcg gcggaagcgg aggcggatcc atggtgagca
     3901 agggcgagga gatggctcgg gaccctcaac ctttcttccc tcctctgtat ctcggaggac
     3961 cagagatcac tactgagaac tgcgagcggg aacctataca tataccaggt tctattcaac
     4021 ctcatggagc actgctcaca gccgatggac attctggcga ggtgctgcaa gtttcactca
     4081 acgcagccac tttcctgggc caggaaccaa ctgttctgcg gggacagacc ctggcagccc
     4141 tgctgccaga ccagtggcct gctctgcaga ctgccctgcc tccaggctgc caggacgccc
     4201 tgcagtatcg cgccaccctg gattggcccg ccgccggaca cctgagcctg accgtgcata
     4261 gggtggccga gctgctgatt ctggaattcg agcctactga ggcatgggac tctattggac
     4321 cccacgctct gagaaatgca atgttcgctc tggagagtgc tcctaatctc cgggcactgg
     4381 ctgaagtcgc aacccaaaca gtccgggaac tgtcaggttt cgaccgggtg atgctgtaca
     4441 agtttgcacc agacgcaaca ggagaggtta ttgccgaagc aaggcgcgag ggcatgcagg
     4501 cttacctcgg gcataggttt cccgcatcca ccacccctgc acaagctagg gccctctaca
     4561 caagacacct gctccggctg accgcagaca ccagggctgc agcagtgccc ctcgaccccg
     4621 tgctgaatcc ccagacaaat gctcctacac ctctgggcgg agctgtcctc agagctacat
     4681 ccccaatgca catgcagtac ctgaggaata tgggagtggg ctcctccctg agcgtcagcg
     4741 tcgtggtcgg cggccagctg tggggactga ttgtctgcca ccatcagaca ccctacgtgc
     4801 tgccaccaga tctgcggacc accctggagt atctggggag gctcctgtcc ctgcaggtgc
     4861 agaggaaaga agccgatatc gagggcagag gaagtctgct aacatgcggt gacgtcgagg
     4921 agaatcctgg cccaatggaa agaccacagc cagactctat gccacaggat ctgagcgaag
     4981 ccctcaagga ggccaccaag gaagtgcaca cccaggccga gaacgctgag ttcatgagaa
     5041 acttccagaa aggacaggtt actcgcgacg gattcaagct ggtgatggct agcctctatc
     5101 acatctacgt ggccctggag gaggaaattg agagaaacaa ggaatccccc gtgttcgctc
     5161 cagtttattt cccagaggag ctgcatcgga aggccgccct ggaacaggat ctggcttttt
     5221 ggtatggccc ccgctggcag gaggtcattc cttatacccc cgctatgcaa cgctacgtta
     5281 agagactcca cgaggtgggc cgcacagaac ctgagctgct cgtggcccat gcctacaccc
     5341 gctacctggg cgacctgtca gggggtcaag ttctgaaaaa gatagcccaa aaggccctgg
     5401 acctgccttc ctctggcgag gggctggcct tctttacttt ccctaacatt gcctcagcca
     5461 caaagttcaa gcagctctac cggtccagga tgaacagcct ggaaatgacc ccagccgtgc
     5521 ggcagcgggt catagaagaa gccaagactg cctttctgct gaatatccag ctgtttgagg
     5581 aactccagga actgctgacc catgacacta aagaccagtc cccctccagg gccccaggac
     5641 tcagacagag ggcctcaaac aaggttcagg actcagcccc cgtggaaaca cccaggggaa
     5701 aaccccctct caatacaaga tctcaagcac ccctgctgag atgggtcctc accctgagct
     5761 tcctcgtcgc taccgttgca gtggggctct atgctatgtg atctagaatt aattaactaa
     5821 gccagtcggc aatcggacgc cttcgaaaca tgcaacacta atgtttaatt cagatttcag
     5881 cagagacaag ctaaaacacc gacgagttgt aatcatttct gtgcgccagc atatatttct
     5941 tatatacaac gtaatacata attatgtaat tctagcatct ccccaacact cacatacaaa
     6001 caaacaaaaa atacaaacac acaaaacgta tttacccgca cgcatccttg gcgaggttga
     6061 gtatgaaaca aaaacaaaac ttaatttaga gcaaagtaat tacacgaata aatttaataa
     6121 aaaaaaacta taataaaaac gccaatcatg ttatttccaa aaaaaaaaaa aaaaaaaaaa
     6181 ctagacgaga tgtttgcgct tgtgtcgatg aatgtaaaga attgcaacta catagatggt
     6241 aattaatttc agctgtaaga agtacaagtt tttcataatt gtgagaaaag tgcagtagta
     6301 tcgcttaaag ttcagtttta atcagggcat ttagcagctc gaacccaatg tatttgtaag
     6361 cccaataaac gtgtaaatgc ctagagcaaa atatcacaac tgtagctttt catccgaaag
     6421 ataataatga atagactgac ctgacgtttt tcaaaatgcg taactagtca cgtaataagt
     6481 gtgcgttgaa tttattcgca aaaacattgc atattttcgg caaagtaaaa ttttgttgca
     6541 taccttatca aaaaataagt gctgcatact ttttagagaa accaaataat tttttattgc
     6601 atacccgttt ttaataaaat acattgcata ccctctttta ataaaaaata ttgcatactt
     6661 tgacgaaaca aattttcgtt gcatacccaa taaaagatta ttatattgca tacccgtttt
     6721 taataaaata cattgcatac cctcttttaa taaaaaatat tgcatacgtt gacgaaacaa
     6781 attttcgttg catacccaat aaaagattat tatattgcat accttttctt gccataccat
     6841 ttagccgatc aattctagta tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga
     6901 taaaaaaaac attttttttt tttactgcac tggatatcat tgaacttatc tgatcagttt
     6961 taaatttact tcgatccaag ggtatttgat gtaccaggtt ctttcgatta cctctcactc
     7021 aaaatgacat tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc
     7081 gccgtctctt tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacgtag
     7141 cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc gtgacgaagc tccaagcggt
     7201 ttacgccatc aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt
     7261 ttgttttttg agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg
     7321 aaaaatcccg gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca
     7381 cccatctgcc gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact
     7441 tgatctatag gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca
     7501 caaaaatccc aaaccgcaat cgcacaaaca aatagtgaca cgaaacagat tattctggta
     7561 gctgttctcg ctatataaga caatttttga gatcatatca tgatcaagac atctaaaggc
     7621 attcattttc gactatattc ttttttacaa aaaatataac aaccagatat tttaagctga
     7681 tcctagatgc acaaaaaata aataaaagta taaacctact tcgtaggata cttcggggta
     7741 ctttttgttc ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca
     7801 ttgcagggtg acagcggagc ggcttcgcag agctgcatta accagggctt cgggcaggcc
     7861 aaaaactacg gcacgctccg gccacccagt ccgccggagg actccggttc agggagcggc
     7921 caactagccg agaacctcac ctatgcctgg cacaatatgg acatctttgg ggcggtcaat
     7981 cagccgggct ccggatggcg gcagctggtc aaccggacac gcggactatt ctgcaacgag
     8041 cgacacatac cggcgcccag gaaacatttg ctcaagaacg gtgagtttct attcgcagtc
     8101 ggctgatctg tgtgaaatct taataaaggg tccaattacc aatttgaaac tcagtttgcg
     8161 gcgtggccta tccgggcgaa cttttggccg tgatgggcag ttccggtgcc ggaaagacga
     8221 ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg ccatccggga
     8281 tgcgactgct caatggccaa cctgtggacg ccaaggagat gcaggccagg tgcgcctatg
     8341 tccagcagga tgacctcttt atcggctccc taacggccag ggaacacctg attttccaag
     8401 ccatggtgcg gatgccacga catctgacct atcggcagcg agtggcccgc gtggatcagg
     8461 tgatccagga gctttcgctc agcaaatgtc agcacacgat catcggtgtg cccggcaggg
     8521 tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt cgcctccgag gctctaaccg
     8581 atccgccgct tctgatctgc gatgagccca cctccggact ggactccttt accgcccaca
     8641 gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc
     8701 atcagccgtc ttccgagctg tttgagctct ttgacaagat ccttctgatg gccgagggca
     8761 gggtagcttt cttgggcact cccagcgaag ccgtcgactt cttttcctag tgagttcgat
     8821 gtgtttatta agggtatcta gtattacata acatctcaac tcctatccag cgtgggtgcc
     8881 cagtgtccta ccaactacaa tccggcggac ttttacgtac aggtgttggc cgttgtgccc
     8941 ggacgggaga tcgagtcccg tgatcggatc gccaagatat gcgacaattt tgccattagc
     9001 aaagtagccc gggatatgga gcagttgttg gccaccaaaa atctggagaa gccactggag
     9061 cagccggaga atgggtacac ctacaaggcc acctggttca tgcagttccg ggcggtcctg
     9121 tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg acttattcag
     9181 acaacggtga gtggttccag tggaaacaaa tgatataacg cttacaattc ttggaaacaa
     9241 attcgctaga ttttagatag aattgcctga ttccacaccc ttcttagttt ttttcaatga
     9301 gatgtatagt ttatagtttt gcagaagata aataaatttc atttaactcg cgaatattaa
     9361 tgagatgcga gtaacatttt aatttgcaga tggttgccat cttgattggc ctcatctttt
     9421 tgggccaaca actcacgcaa gtgggtgtga tgaatatcaa cggagccatc ttcctcttcc
     9481 tgaccaacat gacctttcaa aacgtctttg ccacgataaa tgtaagtcat gtttagaata
     9541 catttgcatt tcaataattt actaactttc taatgaatcg attcgattta ggtgttcacc
     9601 tcagagctgc cagtttttat gagggaggcc cgaagtcgac tttatcgctg tgacacatac
     9661 tttctgggca aaacgattgc cgaattgccg ctttttctca cagtgccact ggtcttcacg
     9721 gcgattgcct atccgatgat cggactgcgg gccggagtgc tgcacttctt caactgcctg
     9781 gcgctggtca ctctggtggc caatgtgtca acgtccttcg gatatctaat atcctgcgcc
     9841 agctcctcga cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc attcctgctc
     9901 tttggcggct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac
     9961 ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag
    10021 ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc
    10081 ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggccatt
    10141 ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag
    10201 gagtagccga catatatccg aaataactgc ttgttttttt tttttaccat tattaccatc
    10261 gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa
    10321 caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca agtagacttt
    10381 ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa aaactcgttt
    10441 atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa tcgcgcgccc
    10501 tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct gtctcccacc
    10561 cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt cgttagttcc
    10621 gcgcgattcg gttcgctcaa atggttccga gtggttcatt tcgtctcaat agaaattagt
    10681 aataaatatt tgtatgtaca atttatttgc tccaatatat ttgtatatat ttccctcaca
    10741 gctatattta ttctaattta atattatgac tttttaaggt aattttttgt gacctgttcg
    10801 gagtgattag cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga
    10861 tgcatctcaa aaaaatggtg ggcataatag tgttgtttat atatatcaaa aataacaact
    10921 ataataataa gaatacattt aatttagaaa atgcttggat ttcactggaa ctagaattaa
    10981 ttcggctgct gctctaaacg acgcatttcg tactccaaag tacgaatttt ttccctcaag
    11041 ctcttatttt cattaaacaa tgaacaggac ctaacgcaca gtcacgttat tgtttacata
    11101 aatgattttt tttactattc aaacttactc tgtttgtgta ctcccactgg tatagccttc
    11161 ttttatcttt tctggttcag gctctatcac tttactaggt acggcatctg cgttgagtcg
    11221 cctcctttta aatgtctgac cttttgcagg tgcagccttc cactgcgaat cattaaagtg
    11281 ggtatcacaa atttgggagt tttcaccaag gctgcaccca aggctctgct cccacaattt
    11341 tctcttaata gcacacttcg gcacgtgaat taattttact ccagtcacag ctttgcagca
    11401 aaatttgcaa tatttcattt ttttttattc cacgtaaggg ttaatgtttt caaaaaaaaa
    11461 ttcgtccgca cacaaccttt cctctcaaca agcaaacgtg cactgaattt aagtgtatac
    11521 ttcggtaagc ttcggctatc gacgggacca ccttatgtta tttcatcatg ggccagaccc
    11581 acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat gaccttgccc
    11641 gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc cacgtccgcc
    11701 cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta cgacaaccat
    11761 ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca taggctccgc
    11821 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    11881 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    11941 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    12001 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    12061 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    12121 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    12181 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    12241 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    12301 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    12361 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    12421 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    12481 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    12541 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    12601 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    12661 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    12721 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    12781 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    12841 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    12901 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    12961 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    13021 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    13081 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    13141 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    13201 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    13261 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    13321 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    13381 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    13441 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    13501 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    13561 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    13621 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
    13681 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
    13741 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
    13801 gtgcaccatt cgacatgccc gccgtgaccg tcgagaaccc gctgacgctg ccccgcgtat
    13861 ccgcacccgc cgacgccgtc gcacgtcccg tgctcaccgt gaccaccgcg cccagcggtt
    13921 tcgagggcga gggcttcccg gtgcgccgcg cgttcgccgg gatcaactac cgccacctcg
    13981 acccgttcat catgatggac cagatgggtg aggtggagta cgcgcccggg gagcccaagg
    14041 gcacgccctg gcacccgcac cgcggcttcg agaccgtgac ctacatcgtc gatatgcggt
    14101 gtgaaatacc gcaccgaatc gcgcggaact aacgacagtc gctccaaggt cgtcgaacaa
    14161 aaggtgaatg tgttgcggag agcgggtggg agacagcgaa agagcaacta cgaaacgtgg
    14221 tgtggtggag gtgaattatg aagagggcgc gcgatttgaa aagtatgtat ataaaaaata
    14281 tatcccggtg ttttatgtag cgataaacga gtttttgatg taaggtatgc aggtgtgtaa
    14341 gtcttttggt tagaagacaa atccaaagtc tacttgtggg gatgttcgaa ggggaaatac
    14401 ttgtattcta taggtcatat cttgttttta ttggcacaaa tataattaca ttagcttttt
    14461 gagggggcaa taaacagtaa acacgatggt aataatggta aaaaaaaaaa acaagcagtt
    14521 atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag ccagatatgc
    14581 gagcacccgg aagctcacga tgagaatggc cagac
//
