LOCUS       pDEST-HemmarG2               14246 bp ds-DNA     circular SYN 02-OCT-2017
DEFINITION  Gateway destination vector for expressing enhancer-driven CD4-tdGFP 
            in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
FEATURES             Location/Qualifiers
     source          1..14246
                     /organism="synthetic DNA construct"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    631..1016
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     misc_feature    1048..2758
                     /label=Gateway RfA cassette
                     /note="Contains attR1 --> attR2"
     protein_bind    1051..1175
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1200..1230
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     misc_feature    complement(1210..1232)
                     /label=ccdB_casR
                     /note="ccdB_casR"
     CDS             1284..1943
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    complement(1352..1373)
                     /label=ccdB_casRII
     CDS             2285..2590
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2631..2755)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     TATA_signal     2772..2974
                     /label=Hsp70
                     /note="TATA box and 5'UTR from Hsp70Bb"
     promoter        2772..2974
                     /label=DSCPm
                     /note="DSCPm: modified Drosophila synthetic core promoter 
                     based on Hsp70"
     misc_feature    2783..2788
                     /label=TATA
                     /note="TATA box"
     misc_feature    2812..2817
                     /label=Inr
                     /note="Inr. A is the transcription starting site (TSS)"
     misc_feature    2831..2840
                     /label=MTE
                     /note="MTE"
     misc_feature    2841..2846
                     /label=DPE
                     /note="DPE"
     intron          2978..3039
                     /gene="zeste"
                     /label=zIn
                     /note="2nd intron of Drosophila zeste gene"
     misc_signal     3072..3092
                     /label=Syn21
                     /note="Syn21 translational enhancer"
     CDS             3093..5432
                     /codon_start=1
                     /label=Akh-CD4-tdGFP-ER
                     /note="Akh-CD4-tdGFP2-ER1"
                     /translation="MNPKSEVLIAAVLFMLLACVQCQASGGGGSGGGGSFQKASSIVYK
                     KEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKL
                     QMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVW
                     GPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTW
                     STPVQPMALIVLGGVAGLLLFIGLGIFFCVRCRHRRRGGSGGGSRSRAQASVSKGEELF
                     TGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYG
                     VQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELK
                     GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQN
                     TPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMGHGTGSTGSGS
                     SGTASSEDNNMASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
                     TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYK
                     TRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFK
                     IRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT
                     AAGITHGMDELYENANSFCYENEVAL"
     sig_peptide     3093..3161
                     /label=Akh SP
                     /note="signal peptide of Drosophila Akh, cleaved after 
                     AA22. modified"
     misc_feature    3168..3197
                     /label=Linker
                     /note="Peptide linker"
     CDS             3198..3869
                     /label=CD4-2
                     /note="CD4-2 CDS"
     misc_feature    3870..3884
                     /label=GS linker
     transit_peptide 3891..3911
                     /label=header
                     /note="header for tdGFP"
     CDS             3912..4610
                     /label=EGFP
                     /note="EGFP"
     transit_peptide 4611..4679
                     /label=linker
                     /note="peptide linker"
     CDS             4680..5387
                     /label=GFP
                     /note="GFP - F64L S65T. GFP has additional nt changes 
                     compared to S65T: codon 93 from GTA to GTC (silent); codon 
                     80 from CGG to CAG (Arg --> Gln);"
     CDS             5391..5429
                     /codon_start=1
                     /label=AA370-382 of Kir2.1
                     /note="AA370-382 of Kir2.1"
                     /translation="NANSFCYENEVAL"
     misc_feature    5403..5423
                     /label=ER export signal
                     /note="ER export signal"
     misc_feature    5448..6093
                     /label=His2Av stop
                     /note="His2Av stop, including 3'UTR and polyA signal"
     3'UTR           5452..5774
                     /label=3'UTR of His2Av
                     /note="3'UTR of His2Av"
     protein_bind    6100..6485
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     CDS             join(6965..7036,7438..7711,7786..8440,8502..8817,9021..9152,
                     9223..9837)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    10616..11201
                     /label=P element 5' end
     misc_feature    11202..11429
                     /label=linker
                     /note="white linker sequences"
     rep_origin      complement(11436..12024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12195..13055)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13056..13160)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    13634..13703
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttggctgcat cacgtaataa gtgtgcgttg aatttattcg
      661 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
      721 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
      781 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
      841 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
      901 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
      961 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     1021 tcggcaacag catgctgcag aagacttatc acaagtttgt acaaaaaagc tgaacgagaa
     1081 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     1141 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
     1201 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     1261 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     1321 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     1381 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1441 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1501 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1561 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1621 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1681 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     1741 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1801 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1861 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1921 atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt
     1981 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     2041 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     2101 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     2161 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     2221 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     2281 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     2341 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     2401 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
     2461 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     2521 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     2581 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     2641 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     2701 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgattt
     2761 gtcaaggtac cgagcgccgg agtataaata gaggcgcttc gtctacggag ctcagttgtg
     2821 aatgaatgtt cgagccgagc agacgtgccg cgcgaaagct aagcaaataa acaagcgcag
     2881 ctgagcaagc taaacaatcg taaagtgcaa gttaaagtga atcaattaaa agtaaccagc
     2941 aaccaagtaa atcaactgca actactgaaa tctgccggtg agattgtaca cccttcttaa
     3001 agatcccctt caaagtctaa attcttcctt ttcgtttagc cactcgagag aaaaccggta
     3061 cgcgtgaatt caacttaaaa aaaaaaatca aaatgaatcc caagagcgaa gtcctcattg
     3121 cagccgtgct gttcatgctg ctggcctgcg tccagtgtca agctagcggt ggcggcggaa
     3181 gtggaggtgg aggctcgttc cagaaggcct ccagcatagt ctataagaaa gagggggaac
     3241 aggtggagtt ctccttccca ctcgccttta cagttgaaaa gctgacgggc agtggcgagc
     3301 tgtggtggca ggcggagagg gcttcctcct ccaagtcttg gatcaccttt gacctgaaga
     3361 acaaggaagt gtctgtaaaa cgggttaccc aggaccctaa gctccagatg ggcaagaagc
     3421 tcccgctcca cctcaccctg ccccaggcct tgcctcagta tgctggctct ggaaacctca
     3481 ccctggccct tgaagcgaaa acaggaaagt tgcatcagga agtgaacctg gtggtgatga
     3541 gagccactca gctccagaaa aatttgacct gtgaggtgtg gggacccacc tcccctaagc
     3601 tgatgctgag cttgaaactg gagaacaagg aggcaaaggt ctcgaagcgg gagaaggcgg
     3661 tgtgggtgct gaaccctgag gcggggatgt ggcagtgtct gctgagtgac tcgggacagg
     3721 tcctgctgga atccaacatc aaggttctgc ccacatggtc caccccggtg cagccaatgg
     3781 ccctgattgt gctggggggc gtcgccggcc tcctgctttt cattgggcta ggcatcttct
     3841 tctgtgtcag gtgccggcac cgaaggcgcg gcggaagcgg aggcggatcc cgtagtcgtg
     3901 cccaggcttc cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3961 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     4021 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     4081 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     4141 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     4201 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     4261 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     4321 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     4381 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     4441 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     4501 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     4561 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gggcatggca
     4621 ccggcagcac cggcagcggc agctccggaa ccgcctcctc cgaggacaac aacatggcca
     4681 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
     4741 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
     4801 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
     4861 ctactttaac ttatggtgtt caatgctttt caagataccc agatcatatg aaacagcatg
     4921 actttttcaa gagtgccatg cccgaaggtt atgtccagga aagaactata tttttcaaag
     4981 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
     5041 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     5101 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
     5161 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
     5221 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     5281 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
     5341 agtttgtaac agctgctggg attacacatg gcatggatga actctacgag aacgcaaaca
     5401 gcttttgcta tgaaaatgaa gttgccctct aatctagaat taattaacta agccagtcgg
     5461 caatcggacg ccttcgaaac atgcaacact aatgtttaat tcagatttca gcagagacaa
     5521 gctaaaacac cgacgagttg taatcatttc tgtgcgccag catatatttc ttatatacaa
     5581 cgtaatacat aattatgtaa ttctagcatc tccccaacac tcacatacaa acaaacaaaa
     5641 aatacaaaca cacaaaacgt atttacccgc acgcatcctt ggcgaggttg agtatgaaac
     5701 aaaaacaaaa cttaatttag agcaaagtaa ttacacgaat aaatttaata aaaaaaaact
     5761 ataataaaaa cgccaatcat gttatttcca aaaaaaaaaa aaaaaaaaaa actagacgag
     5821 atgtttgcgc ttgtgtcgat gaatgtaaag aattgcaact acatagatgg taattaattt
     5881 cagctgtaag aagtacaagt ttttcataat tgtgagaaaa gtgcagtagt atcgcttaaa
     5941 gttcagtttt aatcagggca tttagcagct cgaacccaat gtatttgtaa gcccaataaa
     6001 cgtgtaaatg cctagagcaa aatatcacaa ctgtagcttt tcatccgaaa gataataatg
     6061 aatagactga cctgacgttt ttcaaaatgc gtaactagtc acgtaataag tgtgcgttga
     6121 atttattcgc aaaaacattg catattttcg gcaaagtaaa attttgttgc ataccttatc
     6181 aaaaaataag tgctgcatac tttttagaga aaccaaataa ttttttattg catacccgtt
     6241 tttaataaaa tacattgcat accctctttt aataaaaaat attgcatact ttgacgaaac
     6301 aaattttcgt tgcataccca ataaaagatt attatattgc atacccgttt ttaataaaat
     6361 acattgcata ccctctttta ataaaaaata ttgcatacgt tgacgaaaca aattttcgtt
     6421 gcatacccaa taaaagatta ttatattgca taccttttct tgccatacca tttagccgat
     6481 caattctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
     6541 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
     6601 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
     6661 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
     6721 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
     6781 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     6841 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     6901 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     6961 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     7021 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     7081 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     7141 caaaccgcaa tcgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     7201 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     7261 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     7321 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     7381 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     7441 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     7501 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     7561 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     7621 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     7681 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     7741 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     7801 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     7861 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     7921 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     7981 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     8041 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     8101 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     8161 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     8221 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     8281 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     8341 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     8401 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     8461 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     8521 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     8581 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     8641 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     8701 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     8761 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     8821 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     8881 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     8941 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     9001 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     9061 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     9121 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     9181 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     9241 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     9301 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     9361 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     9421 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     9481 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     9541 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     9601 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     9661 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     9721 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     9781 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     9841 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     9901 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     9961 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
    10021 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
    10081 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
    10141 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
    10201 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
    10261 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
    10321 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
    10381 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
    10441 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
    10501 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
    10561 agaatacatt taatttagaa aatgcttgga tttcactgga actagaatta attcggctgc
    10621 tgctctaaac gacgcatttc gtactccaaa gtacgaattt tttccctcaa gctcttattt
    10681 tcattaaaca atgaacagga cctaacgcac agtcacgtta ttgtttacat aaatgatttt
    10741 ttttactatt caaacttact ctgtttgtgt actcccactg gtatagcctt cttttatctt
    10801 ttctggttca ggctctatca ctttactagg tacggcatct gcgttgagtc gcctcctttt
    10861 aaatgtctga ccttttgcag gtgcagcctt ccactgcgaa tcattaaagt gggtatcaca
    10921 aatttgggag ttttcaccaa ggctgcaccc aaggctctgc tcccacaatt ttctcttaat
    10981 agcacacttc ggcacgtgaa ttaattttac tccagtcaca gctttgcagc aaaatttgca
    11041 atatttcatt tttttttatt ccacgtaagg gttaatgttt tcaaaaaaaa attcgtccgc
    11101 acacaacctt tcctctcaac aagcaaacgt gcactgaatt taagtgtata cttcggtaag
    11161 cttcggctat cgacgggacc accttatgtt atttcatcat gggccagacc cacgtagtcc
    11221 agcggcagat cggcggcgga gaagttaagc gtctccagga tgaccttgcc cgaactgggg
    11281 cacgtggtgt tcgacgatgt gcagctaatt tcgcccggct ccacgtccgc ccattggtta
    11341 atcagcagac cctcgttggc gtaacggaac catgagaggt acgacaacca tttgaggtat
    11401 actggcaccg agcccgagtt caagaagaag gcgtttttcc ataggctccg cccccctgac
    11461 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    11521 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    11581 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    11641 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    11701 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    11761 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    11821 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    11881 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    11941 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    12001 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    12061 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    12121 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    12181 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    12241 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    12301 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    12361 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    12421 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    12481 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    12541 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    12601 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    12661 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    12721 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    12781 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    12841 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    12901 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    12961 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    13021 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    13081 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    13141 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    13201 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    13261 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    13321 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    13381 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    13441 tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct gccccgcgta tccgcacccg
    13501 ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc gcccagcggt ttcgagggcg
    13561 agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta ccgccacctc gacccgttca
    13621 tcatgatgga ccagatgggt gaggtggagt acgcgcccgg ggagcccaag ggcacgccct
    13681 ggcacccgca ccgcggcttc gagaccgtga cctacatcgt cgatatgcgg tgtgaaatac
    13741 cgcaccgaat cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat
    13801 gtgttgcgga gagcgggtgg gagacagcga aagagcaact acgaaacgtg gtgtggtgga
    13861 ggtgaattat gaagagggcg cgcgatttga aaagtatgta tataaaaaat atatcccggt
    13921 gttttatgta gcgataaacg agtttttgat gtaaggtatg caggtgtgta agtcttttgg
    13981 ttagaagaca aatccaaagt ctacttgtgg ggatgttcga aggggaaata cttgtattct
    14041 ataggtcata tcttgttttt attggcacaa atataattac attagctttt tgagggggca
    14101 ataaacagta aacacgatgg taataatggt aaaaaaaaaa aacaagcagt tatttcggat
    14161 atatgtcggc tactccttgc gtcgggcccg aagtcttaga gccagatatg cgagcacccg
    14221 gaagctcacg atgagaatgg ccagac
//
