LOCUS       Exported               14177 bp ds-DNA     circular SYN 02-OCT-2017
DEFINITION  Gateway destination vector for expressing enhancer-driven 
            CD4-tdTomato in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="synthetic DNA construct"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    631..1016
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     misc_feature    1048..2758
                     /label=Gateway RfA cassette
                     /note="Contains attR1 --> attR2"
     protein_bind    1051..1175
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1200..1230
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1284..1943
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2285..2590
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2631..2755)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     TATA_signal     2772..2974
                     /label=Hsp70
                     /note="TATA box and 5'UTR from Hsp70Bb"
     promoter        2772..2974
                     /label=DSCPm
                     /note="DSCPm: modified Drosophila synthetic core promoter 
                     based on Hsp70"
     misc_feature    2783..2788
                     /label=TATA
                     /note="TATA box"
     misc_feature    2812..2817
                     /label=Inr
                     /note="Inr. A is the transcription starting site (TSS)"
     misc_feature    2831..2840
                     /label=MTE
                     /note="MTE"
     misc_feature    2841..2846
                     /label=DPE
                     /note="DPE"
     intron          2978..3039
                     /gene="zeste"
                     /label=zIn
                     /note="2nd intron of Drosophila zeste gene"
     misc_signal     3072..3092
                     /label=Syn21
                     /note="Syn21 translational enhancer"
     sig_peptide     3093..3161
                     /label=Akh SP
                     /note="signal peptide of Drosophila Akh, cleaved after 
                     AA22. modified"
     misc_feature    3168..3197
                     /label=Linker
                     /note="Peptide linker"
     CDS             3198..3869
                     /label=CD4-2
                     /note="CD4-2 CDS"
     misc_feature    3870..3884
                     /label=GS linker
     CDS             3891..5318
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    5379..6024
                     /label=His2Av stop
                     /note="His2Av stop, including 3'UTR and polyA signal"
     3'UTR           5383..5705
                     /label=3'UTR of His2Av
                     /note="3'UTR of His2Av"
     misc_feature    5669..5674
                     /label=polyA
                     /note="polyA signal predicted by Softberry-FGENESH"
     protein_bind    6031..6416
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     CDS             join(6896..6967,7369..7642,7717..8371,8433..8748,8952..9083,
                     9154..9768)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    10547..11132
                     /label=P element 5' end
     rep_origin      complement(11367..11955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12126..12986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12987..13091)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    complement(13565..13634)
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttggctgcat cacgtaataa gtgtgcgttg aatttattcg
      661 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
      721 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
      781 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
      841 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
      901 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
      961 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     1021 tcggcaacag catgctgcag aagacttatc acaagtttgt acaaaaaagc tgaacgagaa
     1081 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     1141 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
     1201 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     1261 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     1321 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     1381 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     1441 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1501 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1561 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1621 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1681 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     1741 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1801 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1861 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1921 atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt
     1981 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     2041 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     2101 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     2161 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     2221 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     2281 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     2341 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     2401 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
     2461 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     2521 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     2581 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     2641 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     2701 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgattt
     2761 gtcaaggtac cgagcgccgg agtataaata gaggcgcttc gtctacggag ctcagttgtg
     2821 aatgaatgtt cgagccgagc agacgtgccg cgcgaaagct aagcaaataa acaagcgcag
     2881 ctgagcaagc taaacaatcg taaagtgcaa gttaaagtga atcaattaaa agtaaccagc
     2941 aaccaagtaa atcaactgca actactgaaa tctgccggtg agattgtaca cccttcttaa
     3001 agatcccctt caaagtctaa attcttcctt ttcgtttagc cactcgagag aaaaccggta
     3061 cgcgtgaatt caacttaaaa aaaaaaatca aaatgaatcc caagagcgaa gtcctcattg
     3121 cagccgtgct gttcatgctg ctggcctgcg tccagtgtca agctagcggt ggcggcggaa
     3181 gtggaggtgg aggctcgttc cagaaggcct ccagcatagt ctataagaaa gagggggaac
     3241 aggtggagtt ctccttccca ctcgccttta cagttgaaaa gctgacgggc agtggcgagc
     3301 tgtggtggca ggcggagagg gcttcctcct ccaagtcttg gatcaccttt gacctgaaga
     3361 acaaggaagt gtctgtaaaa cgggttaccc aggaccctaa gctccagatg ggcaagaagc
     3421 tcccgctcca cctcaccctg ccccaggcct tgcctcagta tgctggctct ggaaacctca
     3481 ccctggccct tgaagcgaaa acaggaaagt tgcatcagga agtgaacctg gtggtgatga
     3541 gagccactca gctccagaaa aatttgacct gtgaggtgtg gggacccacc tcccctaagc
     3601 tgatgctgag cttgaaactg gagaacaagg aggcaaaggt ctcgaagcgg gagaaggcgg
     3661 tgtgggtgct gaaccctgag gcggggatgt ggcagtgtct gctgagtgac tcgggacagg
     3721 tcctgctgga atccaacatc aaggttctgc ccacatggtc caccccggtg cagccaatgg
     3781 ccctgattgt gctggggggc gtcgccggcc tcctgctttt cattgggcta ggcatcttct
     3841 tctgtgtcag gtgccggcac cgaaggcgcg gcggaagcgg aggcggatcc atggtgagca
     3901 agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga
     3961 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     4021 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     4081 cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca
     4141 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     4201 gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga
     4261 agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct
     4321 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc
     4381 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg
     4441 ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct
     4501 cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc
     4561 tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc gcctcctccg
     4621 aggacaacaa catggccgtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct
     4681 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     4741 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
     4801 tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg
     4861 attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     4921 acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca
     4981 aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca
     5041 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga
     5101 tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct
     5161 acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca
     5221 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc
     5281 accacctgtt cctgtacggc atggacgagc tgtacaagga gaacgcaaac agcttttgct
     5341 atgaaaatga agttgccctc taatctagaa ttaattaact aagccagtcg gcaatcggac
     5401 gccttcgaaa catgcaacac taatgtttaa ttcagatttc agcagagaca agctaaaaca
     5461 ccgacgagtt gtaatcattt ctgtgcgcca gcatatattt cttatataca acgtaataca
     5521 taattatgta attctagcat ctccccaaca ctcacataca aacaaacaaa aaatacaaac
     5581 acacaaaacg tatttacccg cacgcatcct tggcgaggtt gagtatgaaa caaaaacaaa
     5641 acttaattta gagcaaagta attacacgaa taaatttaat aaaaaaaaac tataataaaa
     5701 acgccaatca tgttatttcc aaaaaaaaaa aaaaaaaaaa aactagacga gatgtttgcg
     5761 cttgtgtcga tgaatgtaaa gaattgcaac tacatagatg gtaattaatt tcagctgtaa
     5821 gaagtacaag tttttcataa ttgtgagaaa agtgcagtag tatcgcttaa agttcagttt
     5881 taatcagggc atttagcagc tcgaacccaa tgtatttgta agcccaataa acgtgtaaat
     5941 gcctagagca aaatatcaca actgtagctt ttcatccgaa agataataat gaatagactg
     6001 acctgacgtt tttcaaaatg cgtaactagt cacgtaataa gtgtgcgttg aatttattcg
     6061 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
     6121 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
     6181 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
     6241 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
     6301 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
     6361 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattctag
     6421 tatgtatgta agttaataaa acccattttt gcggaaagta gataaaaaaa acattttttt
     6481 tttttactgc actggatatc attgaactta tctgatcagt tttaaattta cttcgatcca
     6541 agggtatttg atgtaccagg ttctttcgat tacctctcac tcaaaatgac attccactca
     6601 aagtcagcgc tgtttgcctc cttctctgtc cacagaaata tcgccgtctc tttcgccgct
     6661 gcgtccgcta tctctttcgc caccgtttgt agcgttacgt agcgtcaatg tccgccttca
     6721 gttgcatttt gtcagcggtt tcgtgacgaa gctccaagcg gtttacgcca tcaattaaac
     6781 acaaagtgct gtgccaaaac tcctctcgct tcttattttt gtttgttttt tgagtgattg
     6841 gggtggtgat tggttttggg tgggtaagca ggggaaagtg tgaaaaatcc cggcaatggg
     6901 ccaagaggat caggagctat taattcgcgg aggcagcaaa cacccatctg ccgagcatct
     6961 gaacaatgtg agtagtacat gtgcatacat cttaagttca cttgatctat aggaactgcg
     7021 attgcaacat caaattgtct gcggcgtgag aactgcgacc cacaaaaatc ccaaaccgca
     7081 atcgcacaaa caaatagtga cacgaaacag attattctgg tagctgttct cgctatataa
     7141 gacaattttt gagatcatat catgatcaag acatctaaag gcattcattt tcgactatat
     7201 tcttttttac aaaaaatata acaaccagat attttaagct gatcctagat gcacaaaaaa
     7261 taaataaaag tataaaccta cttcgtagga tacttcgggg tactttttgt tcggggttag
     7321 atgagcataa cgcttgtagt tgatatttga gatcccctat cattgcaggg tgacagcgga
     7381 gcggcttcgc agagctgcat taaccagggc ttcgggcagg ccaaaaacta cggcacgctc
     7441 cggccaccca gtccgccgga ggactccggt tcagggagcg gccaactagc cgagaacctc
     7501 acctatgcct ggcacaatat ggacatcttt ggggcggtca atcagccggg ctccggatgg
     7561 cggcagctgg tcaaccggac acgcggacta ttctgcaacg agcgacacat accggcgccc
     7621 aggaaacatt tgctcaagaa cggtgagttt ctattcgcag tcggctgatc tgtgtgaaat
     7681 cttaataaag ggtccaatta ccaatttgaa actcagtttg cggcgtggcc tatccgggcg
     7741 aacttttggc cgtgatgggc agttccggtg ccggaaagac gaccctgctg aatgcccttg
     7801 cctttcgatc gccgcagggc atccaagtat cgccatccgg gatgcgactg ctcaatggcc
     7861 aacctgtgga cgccaaggag atgcaggcca ggtgcgccta tgtccagcag gatgacctct
     7921 ttatcggctc cctaacggcc agggaacacc tgattttcca agccatggtg cggatgccac
     7981 gacatctgac ctatcggcag cgagtggccc gcgtggatca ggtgatccag gagctttcgc
     8041 tcagcaaatg tcagcacacg atcatcggtg tgcccggcag ggtgaaaggt ctgtccggcg
     8101 gagaaaggaa gcgtctggca ttcgcctccg aggctctaac cgatccgccg cttctgatct
     8161 gcgatgagcc cacctccgga ctggactcct ttaccgccca cagcgtcgtc caggtgctga
     8221 agaagctgtc gcagaagggc aagaccgtca tcctgaccat tcatcagccg tcttccgagc
     8281 tgtttgagct ctttgacaag atccttctga tggccgaggg cagggtagct ttcttgggca
     8341 ctcccagcga agccgtcgac ttcttttcct agtgagttcg atgtgtttat taagggtatc
     8401 tagtattaca taacatctca actcctatcc agcgtgggtg cccagtgtcc taccaactac
     8461 aatccggcgg acttttacgt acaggtgttg gccgttgtgc ccggacggga gatcgagtcc
     8521 cgtgatcgga tcgccaagat atgcgacaat tttgccatta gcaaagtagc ccgggatatg
     8581 gagcagttgt tggccaccaa aaatctggag aagccactgg agcagccgga gaatgggtac
     8641 acctacaagg ccacctggtt catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg
     8701 gtgctcaagg aaccactcct cgtaaaagtg cgacttattc agacaacggt gagtggttcc
     8761 agtggaaaca aatgatataa cgcttacaat tcttggaaac aaattcgcta gattttagat
     8821 agaattgcct gattccacac ccttcttagt ttttttcaat gagatgtata gtttatagtt
     8881 ttgcagaaga taaataaatt tcatttaact cgcgaatatt aatgagatgc gagtaacatt
     8941 ttaatttgca gatggttgcc atcttgattg gcctcatctt tttgggccaa caactcacgc
     9001 aagtgggtgt gatgaatatc aacggagcca tcttcctctt cctgaccaac atgacctttc
     9061 aaaacgtctt tgccacgata aatgtaagtc atgtttagaa tacatttgca tttcaataat
     9121 ttactaactt tctaatgaat cgattcgatt taggtgttca cctcagagct gccagttttt
     9181 atgagggagg cccgaagtcg actttatcgc tgtgacacat actttctggg caaaacgatt
     9241 gccgaattgc cgctttttct cacagtgcca ctggtcttca cggcgattgc ctatccgatg
     9301 atcggactgc gggccggagt gctgcacttc ttcaactgcc tggcgctggt cactctggtg
     9361 gccaatgtgt caacgtcctt cggatatcta atatcctgcg ccagctcctc gacctcgatg
     9421 gcgctgtctg tgggtccgcc ggttatcata ccattcctgc tctttggcgg cttcttcttg
     9481 aactcgggct cggtgccagt atacctcaaa tggttgtcgt acctctcatg gttccgttac
     9541 gccaacgagg gtctgctgat taaccaatgg gcggacgtgg agccgggcga aattagctgc
     9601 acatcgtcga acaccacgtg ccccagttcg ggcaaggtca tcctggagac gcttaacttc
     9661 tccgccgccg atctgccgct ggactacgtg ggtctggcca ttctcatcgt gagcttccgg
     9721 gtgctcgcat atctggctct aagacttcgg gcccgacgca aggagtagcc gacatatatc
     9781 cgaaataact gcttgttttt tttttttacc attattacca tcgtgtttac tgtttattgc
     9841 cccctcaaaa agctaatgta attatatttg tgccaataaa aacaagatat gacctataga
     9901 atacaagtat ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca
     9961 aaagacttac acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc
    10021 gggatatatt ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc
    10081 accacaccac gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt
    10141 caccttttgt tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggttcgctc
    10201 aaatggttcc gagtggttca tttcgtctca atagaaatta gtaataaata tttgtatgta
    10261 caatttattt gctccaatat atttgtatat atttccctca cagctatatt tattctaatt
    10321 taatattatg actttttaag gtaatttttt gtgacctgtt cggagtgatt agcgttacaa
    10381 tttgaactga aagtgacatc cagtgtttgt tccttgtgta gatgcatctc aaaaaaatgg
    10441 tgggcataat agtgttgttt atatatatca aaaataacaa ctataataat aagaatacat
    10501 ttaatttaga aaatgcttgg atttcactgg aactagaatt aattcggctg ctgctctaaa
    10561 cgacgcattt cgtactccaa agtacgaatt ttttccctca agctcttatt ttcattaaac
    10621 aatgaacagg acctaacgca cagtcacgtt attgtttaca taaatgattt tttttactat
    10681 tcaaacttac tctgtttgtg tactcccact ggtatagcct tcttttatct tttctggttc
    10741 aggctctatc actttactag gtacggcatc tgcgttgagt cgcctccttt taaatgtctg
    10801 accttttgca ggtgcagcct tccactgcga atcattaaag tgggtatcac aaatttggga
    10861 gttttcacca aggctgcacc caaggctctg ctcccacaat tttctcttaa tagcacactt
    10921 cggcacgtga attaatttta ctccagtcac agctttgcag caaaatttgc aatatttcat
    10981 ttttttttat tccacgtaag ggttaatgtt ttcaaaaaaa aattcgtccg cacacaacct
    11041 ttcctctcaa caagcaaacg tgcactgaat ttaagtgtat acttcggtaa gcttcggcta
    11101 tcgacgggac caccttatgt tatttcatca tgggccagac ccacgtagtc cagcggcaga
    11161 tcggcggcgg agaagttaag cgtctccagg atgaccttgc ccgaactggg gcacgtggtg
    11221 ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg cccattggtt aatcagcaga
    11281 ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc atttgaggta tactggcacc
    11341 gagcccgagt tcaagaagaa ggcgtttttc cataggctcc gcccccctga cgagcatcac
    11401 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    11461 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    11521 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    11581 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    11641 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    11701 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    11761 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    11821 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    11881 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    11941 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    12001 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    12061 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    12121 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    12181 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    12241 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    12301 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    12361 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    12421 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    12481 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    12541 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    12601 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    12661 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    12721 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
    12781 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    12841 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    12901 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    12961 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    13021 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    13081 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    13141 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    13201 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    13261 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    13321 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca ttcgacatgc
    13381 ccgccgtgac cgtcgagaac ccgctgacgc tgccccgcgt atccgcaccc gccgacgccg
    13441 tcgcacgtcc cgtgctcacc gtgaccaccg cgcccagcgg tttcgagggc gagggcttcc
    13501 cggtgcgccg cgcgttcgcc gggatcaact accgccacct cgacccgttc atcatgatgg
    13561 accagatggg tgaggtggag tacgcgcccg gggagcccaa gggcacgccc tggcacccgc
    13621 accgcggctt cgagaccgtg acctacatcg tcgatatgcg gtgtgaaata ccgcaccgaa
    13681 tcgcgcggaa ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa tgtgttgcgg
    13741 agagcgggtg ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg aggtgaatta
    13801 tgaagagggc gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg tgttttatgt
    13861 agcgataaac gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg gttagaagac
    13921 aaatccaaag tctacttgtg gggatgttcg aaggggaaat acttgtattc tataggtcat
    13981 atcttgtttt tattggcaca aatataatta cattagcttt ttgagggggc aataaacagt
    14041 aaacacgatg gtaataatgg taaaaaaaaa aaacaagcag ttatttcgga tatatgtcgg
    14101 ctactccttg cgtcgggccc gaagtcttag agccagatat gcgagcaccc ggaagctcac
    14161 gatgagaatg gccagac
//
