LOCUS       Exported                2520 bp ds-DNA     circular SYN 06-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    P251_pMGC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 6, 2017 from SnapGene 3.3.2
            http://www.snapgene.com
COMMENT     pDONR221
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /note="rrnB T2 transcription termination"
                     /note="rrnB T2 transcription termination sequence"
     terminator      complement(427..470)
                     /note="rrnB T1 transcription termination"
                     /note="rrnB T1 transcription termination sequence"
     misc_feature    508..530
                     /note="O378_ENTR221_F"
     primer_bind     537..552
                     /note="M13 Forward primer"
     misc_feature    561..757
                     /note="Untitled"
     misc_feature    579..664
                     /note="gRNA core EF"
     tRNA            665..741
                     /note="At tRNA Gly"
     promoter        complement(758..777)
                     /note="T7 promoter"
     primer_bind     complement(785..801)
                     /note="M13 reverse primer"
     misc_feature    complement(808..829)
                     /note="O379_ENTR221_R"
     gene            914..1723
                     /note="Kanamycin resistance gene"
     rep_origin      1844..2517
                     /direction=RIGHT
                     /note="pUC origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccgct cttcacgggt ttaagagcta tgctggaaac
      601 agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      661 gtgcaacaaa gcaccagtgg tctagtggta gaatagtacc ctgccacggt acagacccgg
      721 gttcgattcc cggctggtgc aggaagaaga gcctgatatc ccctatagtg agtcgtatta
      781 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
      841 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
      901 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
      961 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     1021 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     1081 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     1141 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     1201 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     1261 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     1321 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     1381 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     1441 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     1501 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     1561 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     1621 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     1681 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     1741 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     1801 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1861 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1921 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1981 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2041 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2101 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2161 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2221 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2281 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2341 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2401 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2461 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
