LOCUS       Exported                8975 bp ds-DNA     circular SYN 06-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    P250_pAC-U63-tgRNA(Rev)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 6, 2017 from SnapGene 3.3.2
            http://www.snapgene.com
COMMENT     pAC-sm (short MCS): pAC+ was digested with EcoRI/SfiI, blunt and 
            ligated.
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          806..1038
                     /note="P3'"
     source          6149..6735
                     /note="P5'"
                     /note="P element 5' end"
     source          6964..8974
                     /note="pUC8 plasmid DNA"
                     /note="from pUC8 backbone"
     misc_recomb     1..283
                     /note="attB"
                     /note="attB with the same orientation as in p[acman]."
     misc_feature    304..805
                     /note="w linker"
                     /note="w[+]car6.1_3', patial seqence of w last exon and 3 
                     'UTR"
     misc_feature    1027..1050
                     /note="O382_pAC-P3_HiTm_R"
     misc_feature    1109..1996
                     /note="Untitled"
     promoter        1132..1552
                     /note="U6:3 promoter"
     misc_feature    1135..1156
                     /note="O288_pU6.3_seqF"
     tRNA            1553..1649
                     /note="Dm tRNA Gly"
     misc_feature    1650..1674
                     /note="SapI adaptor"
     misc_feature    1675..1760
                     /note="gRNA core EF"
     misc_feature    1761..1995
                     /note="U6 genes 3' flanking sequence"
     misc_feature    complement(1774..1794)
                     /note="O279_pU6.3_seqR"
     gene            2012..2834
                     /note="First w gene fragment"
                     /note="The sequence from flybase pCaSpeR4 is not completely
                     correct"
     gene            2012..2834
                     /note="First w gene fragment"
                     /note="First w gene fragment, including mini-promoter, 
                     first exon, and partial first intron, from w+0.8"
     misc_feature    complement(2222..2251)
                     /note="O381_miniW_HiTm_F"
     gene            2839..6139
                     /note="w-II"
                     /note="Second w gene fragment, including partial first 
                     intron and all other exons & introns and 3' flanking 
                     genomic region. From w+3.3"
     misc_feature    6736..6963
                     /note="w linker"
                     /note="w[+]car6.1_5', partial sequence of w last exon, 
                     inversed"
ORIGIN
        1 tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct gccccgcgta tccgcacccg
       61 ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc gcccagcggt ttcgagggcg
      121 agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta ccgccacctc gacccgttca
      181 tcatgatgga ccagatgggt gaggtggagt acgcgcccgg ggagcccaag ggcacgccct
      241 ggcacccgca ccgcggcttc gagaccgtga cctacatcgt cgatatgcgg tgtgaaatac
      301 cgcaccgaat cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat
      361 gtgttgcgga gagcgggtgg gagacagcga aagagcaact acgaaacgtg gtgtggtgga
      421 ggtgaattat gaagagggcg cgcgatttga aaagtatgta tataaaaaat atatcccggt
      481 gttttatgta gcgataaacg agtttttgat gtaaggtatg caggtgtgta agtcttttgg
      541 ttagaagaca aatccaaagt ctacttgtgg ggatgttcga aggggaaata cttgtattct
      601 ataggtcata tcttgttttt attggcacaa atataattac attagctttt tgagggggca
      661 ataaacagta aacacgatgg taataatggt aaaaaaaaaa acaagcagtt atttcggata
      721 tatgtcggct actccttgcg tcgggcccga agtcttagag ccagatatgc gagcacccgg
      781 aagctcacga tgagaatggc cagaccatga tgaaataaca taaggtggtc ccgtcggcaa
      841 gagacatcca cttaacgtat gcttgcaata agtgcgagtg aaaggaatag tattctgagt
      901 gtcgtattga gtctgagtga gacagcgata tgattgttga ttaaccctta gcatgtccgt
      961 ggggtttgaa ttaactcata atattaatta gacgaaatta tttttaaagt tttattttta
     1021 ataatttgcg agtacgcaaa gcttggctgc aggtcgacct cgaggcctcg agttaacgtt
     1081 acgttaacgt taacgttcga ggtcgactct agtggatctg gatctgaatt cttttttgct
     1141 cacctgtgat tgctcctact caaatacaaa aacatcaaat tttctgtcaa taaagcatat
     1201 ttatttatat ttattttaca ggaaagaatt ccttttaaag tgtattttaa cctataatga
     1261 aaaacgatta aaaaaaatac ataaaataat tcgaaaattt ttgaatagcc caggttgata
     1321 aaaattcatt tcatacgttt tataacttat gcccctaagt attttttgac catagtgttt
     1381 caattctaca ttaattttac agagtagaat gaaacgccac ctactcagcc aagaggcgaa
     1441 aaggttagct cgccaagcag agagggcgcc agtgctcact actttttata attctcaact
     1501 tctttttcca gactcagttc gtatatatag acctattttc aatttaacgt cggggctttg
     1561 agtgtgtgta gacatcaagc atcggtggtt cagtggtaga atgctcgcct gccacgcggg
     1621 cggcccgggt tcgattcccg gccgatgcac aaggaagagc gaggctcttc ttgtgtttaa
     1681 gagctatgct ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa
     1741 aagtggcacc gagtcggtgc tttttttgcc tacctggagc ctgagagttg ttcaataaaa
     1801 taaaaatgtt tcgttttttt gctttcgcca gtatttatta tttttcatca atatgtattc
     1861 aatttggtat gtatttagta attgtaatat atagacaatg gttttccgtt gacgtacata
     1921 catctgacgt gtgtttattt agacataata gttatgtttt cacatctttt taatgttcgc
     1981 ttaatgcgta tgcattctag tggcctaatt ctagtatgta tgtaagttaa taaaacccat
     2041 ttttgcggaa agtagataaa aaaaacattt ttttttttta ctgcactgga tatcattgaa
     2101 cttatctgat cagttttaaa tttacttcga tccaagggta tttgatgtac caggttcttt
     2161 cgattacctc tcactcaaaa tgacattcca ctcaaagtca gcgctgtttg cctccttctc
     2221 tgtccacaga aatatcgccg tctctttcgc cgctgcgtcc gctatctctt tcgccaccgt
     2281 ttgtagcgtt acgtagcgtc aatgtccgcc ttcagttgca ttttgtcagc ggtttcgtga
     2341 cgaagctcca agcggtttac gccatcaatt aaacacaaag tgctgtgcca aaactcctct
     2401 cgcttcttat ttttgtttgt tttttgagtg attggggtgg tgattggttt tgggtgggta
     2461 agcaggggaa agtgtgaaaa atcccggcaa tgggccaaga ggatcaggag ctattaattc
     2521 gcggaggcag caaacaccca tctgccgagc atctgaacaa tgtgagtagt acatgtgcat
     2581 acatcttaag ttcacttgat ctataggaac tgcgattgca acatcaaatt gtctgcggcg
     2641 tgagaactgc gacccacaaa aatcccaaac cgcaatcgca caaacaaata gtgacacgaa
     2701 acagattatt ctggtagctg tgctcgctat ataagacaat ttttaagatc atatcatgat
     2761 caagacatct aaaggcattc attttcgact acattctttt ttacaaaaaa tataacaacc
     2821 agatatttta agctgatcct agatgcacaa aaaataaata aaagtataaa cctacttcgt
     2881 aggatacttc gttttgttcg gggttagatg agcataacgc ttgtagttga tatttgagat
     2941 cccctatcat tgcagggtga cagcggagcg gcttcgcaga gctgcattaa ccagggcttc
     3001 gggcaggcca aaaactacgg cacgctcctg ccacccagtc cgccggagga ctccggttca
     3061 gggagcggcc aactagccga gaacctcacc tatgcctggc acaatatgga catctttggg
     3121 gcggtcaatc agccgggctc cggatggcgg cagctggtca accggacacg cggactattc
     3181 tgcaacgagc gacacatacc ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta
     3241 ttcgcagtcg gctgatctgt gtgaaatctt aataaagggt ccaattacca atttgaaact
     3301 cagtttgcgg cgtggcctat ccgggcgaac ttttggccgt gatgggcagt tccggtgccg
     3361 gaaagacgac cctgctgaat gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc
     3421 catccgggat gcgactgctc aatggccaac ctgtggacgc caaggagatg caggccaggt
     3481 gcgcctatgt ccagcaggat gacctcttta tcggctccct aacggccagg gaacacctga
     3541 ttttccaggc catggtgcgg atgccacgac atctgaccta tcggcagcga gtggcccgcg
     3601 tggatcaggt gatccaggag ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc
     3661 ccggcagggt gaaaggtctg tccggcggag aaaggaagcg tctggcattc gcctccgagg
     3721 cactaaccga tccgccgctt ctgatctgcg atgagcccac ctccggactg gactcattta
     3781 ccgcccacag cgtcgtccag gtgctgaaga agctgtcgca gaagggcaag accgtcatcc
     3841 tgaccattca tcagccgtct tccgagctgt ttgagctctt tgacaagatc cttctgatgg
     3901 ccgagggcag ggtagctttc ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt
     3961 gagttcgatg tgtttattaa gggtatctag cattacatta catctcaact cctatccagc
     4021 gtgggtgccc agtgtcctac caactacaat ccggcggact tttacgtaca ggtgttggcc
     4081 gttgtgcccg gacgggagat cgagtcccgt gatcggatcg ccaagatatg cgacaatttt
     4141 gctattagca aagtagcccg ggatatggag cagttgttgg ccaccaaaaa tttggagaag
     4201 ccactggagc agccggagaa tgggtacacc tacaaggcca cctggttcat gcagttccgg
     4261 gcggtcctgt ggcgatcctg gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga
     4321 cttattcaga caacggtgag tggttccagt ggaaacaaat gatataacgc ttacaattct
     4381 tggaaacaaa ttcgctagat tttagttaga attgcctgat tccacaccct tcttagtttt
     4441 tttcaatgag atgtatagtt tatagttttg cagaaaataa ataaatttca tttaactcgc
     4501 gaacatgttg aagatatgaa tattaatgag atgcgagtaa cattttaatt tgcagatggt
     4561 tgccatcttg attggcctca tctttttggg ccaacaactc acgcaagtgg gcgtgatgaa
     4621 tatcaacgga gccatcttcc tcttcctgac caacatgacc tttcaaaacg tctttgccac
     4681 gataaatgta agtcttgttt agaatacatt tgcatattaa taatttacta actttctaat
     4741 gaatcgattc gatttaggtg ttcacctcag agctgccagt ttttatgagg gaggcccgaa
     4801 gtcgacttta tcgctgtgac acatactttc tgggcaaaac gattgccgaa ttaccgcttt
     4861 ttctcacagt gccactggtc ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg
     4921 gagtgctgca cttcttcaac tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt
     4981 ccttcggata tctaatatcc tgcgccagct cctcgacctc gatggcgctg tctgtgggtc
     5041 cgccggttat cataccattc ctgctctttg gcggcttctt cttgaactcg ggctcggtgc
     5101 cagtatacct caaatggttg tcgtacctct catggttccg ttacgccaac gagggtctgc
     5161 tgattaacca atgggcggac gtggagccgg gcgaaattag ctgcacatcg tcgaacacca
     5221 cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa cttctccgcc gccgatctgc
     5281 cgctggacta cgtgggtctg gccattctca tcgtgagctt ccgggtgctc gcatatctgg
     5341 ctctaagact tcgggcccga cgcaaggagt agccgacata tatccgaaat aactgcttgt
     5401 tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat
     5461 gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct
     5521 tcgaacatcc ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg
     5581 cataccttac atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata
     5641 tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt
     5701 agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga
     5761 ccttggagcg actgtcgtta gttccgcgcg attcggttcg ctcaaatggt tccgagtggt
     5821 tcatttcgtc tcaatagaaa ttagtaataa atatttgtat gtacaattta tttgctccaa
     5881 tatatttgta tatatttccc tcacagctat atttattcta atttaatatt atgacttttt
     5941 aaggtaattt tttgtgacct gttcggagtg attagcgtta caatttgaac tgaaagtgac
     6001 atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg
     6061 tttatatata tcaaaaataa caactataat aataagaata catttaattt agaaaatgct
     6121 tggatttcac tggaactaga attaattcgg ctgctgctct aaacgacgca tttcgtactc
     6181 caaagtacga attttttccc tcaagctctt attttcatta aacaatgaac aggacctaac
     6241 gcacagtcac gttattgttt acataaatga ttttttttac tattcaaact tactctgttt
     6301 gtgtactccc actggtatag ccttctttta tcttttctgg ttcaggctct atcactttac
     6361 taggtacggc atctgcgttg agtcgcctcc ttttaaatgt ctgacctttt gcaggtgcag
     6421 ccttccactg cgaatcatta aagtgggtat cacaaatttg ggagttttca ccaaggctgc
     6481 acccaaggct ctgctcccac aattttctct taatagcaca cttcggcacg tgaattaatt
     6541 ttactccagt cacagctttg cagcaaaatt tgcaatattt catttttttt tattccacgt
     6601 aagggttaat gttttcaaaa aaaaattcgt ccgcacacaa cctttcctct caacaagcaa
     6661 acgtgcactg aatttaagtg tatacttcgg taagcttcgg ctatcgacgg gaccacctta
     6721 tgttatttca tcatgggcca gacccacgta gtccagcggc agatcggcgg cggagaagtt
     6781 aagcgtctcc aggatgacct tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct
     6841 aatttcgccc ggctccacgt ccgcccattg gttaatcagc agaccctcgt tggcgtaacg
     6901 gaaccatgag aggtacgaca accatttgag gtatactggc accgagcccg agttcaagaa
     6961 gaaggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7021 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7081 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7141 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
     7201 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7261 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7321 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7381 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     7441 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7501 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7561 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7621 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7681 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7741 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7801 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7861 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7921 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7981 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8041 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8101 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8161 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8221 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8281 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8341 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8401 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8461 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8521 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8581 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8641 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8701 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     8761 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     8821 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     8881 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     8941 ggcatcagag cagattgtac tgagagtgca ccata
//
