LOCUS       Exported                9237 bp ds-DNA     circular SYN 14-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    P164_pAC-U61-SapI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 14, 2019 from SnapGene 3.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    194..263
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    304..805
                     /note="w linker"
                     /note="w[+]car6.1_3', patial seqence of w last exon and 3 
                     'UTR"
     misc_feature    806..1038
                     /note="P element 3' end"
     misc_feature    1027..1050
                     /note="O382_pAC-P3_HiTm_R"
     misc_feature    complement(1114..1347)
                     /note="U6 genes 3' flanking sequence"
     misc_feature    1315..1335
                     /note="O279_pU6.3_seqR"
                     /note="pU6.3_seqR"
     misc_feature    complement(1348..1423)
                     /note="gRNA core"
     misc_feature    complement(1424..1442)
                     /note="SapI adaptor"
     promoter        complement(1443..2239)
                     /note="U6:1 promoter"
     misc_feature    complement(1443)
                     /note="gRNA transcript start"
     misc_feature    complement(1543..1563)
                     /note="O289_pU6.1_seqF"
     misc_feature    complement(1565..1592)
                     /note="O455_U61_HiTm_F"
     gene            2274..3096
                     /note="w-I"
                     /note="The sequence from flybase pCaSpeR4 is not completely
                     correct"
     misc_feature    complement(2484..2513)
                     /note="O381_miniW_HiTm_F"
     CDS             join(2752..2823,3218..3491,3566..4220,4282..4597,4818..4949,
                     5020..5634)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /note="mini-white"
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    6412..6997
                     /note="P element 5' end"
     misc_feature    6998..7225
                     /note="w linker"
                     /note="w[+]car6.1_5', partial sequence of w last exon, 
                     inversed"
     rep_origin      complement(7232..7820)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7991..8851)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8852..8956)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct gccccgcgta tccgcacccg
       61 ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc gcccagcggt ttcgagggcg
      121 agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta ccgccacctc gacccgttca
      181 tcatgatgga ccagatgggt gaggtggagt acgcgcccgg ggagcccaag ggcacgccct
      241 ggcacccgca ccgcggcttc gagaccgtga cctacatcgt cgatatgcgg tgtgaaatac
      301 cgcaccgaat cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat
      361 gtgttgcgga gagcgggtgg gagacagcga aagagcaact acgaaacgtg gtgtggtgga
      421 ggtgaattat gaagagggcg cgcgatttga aaagtatgta tataaaaaat atatcccggt
      481 gttttatgta gcgataaacg agtttttgat gtaaggtatg caggtgtgta agtcttttgg
      541 ttagaagaca aatccaaagt ctacttgtgg ggatgttcga aggggaaata cttgtattct
      601 ataggtcata tcttgttttt attggcacaa atataattac attagctttt tgagggggca
      661 ataaacagta aacacgatgg taataatggt aaaaaaaaaa acaagcagtt atttcggata
      721 tatgtcggct actccttgcg tcgggcccga agtcttagag ccagatatgc gagcacccgg
      781 aagctcacga tgagaatggc cagaccatga tgaaataaca taaggtggtc ccgtcggcaa
      841 gagacatcca cttaacgtat gcttgcaata agtgcgagtg aaaggaatag tattctgagt
      901 gtcgtattga gtctgagtga gacagcgata tgattgttga ttaaccctta gcatgtccgt
      961 ggggtttgaa ttaactcata atattaatta gacgaaatta tttttaaagt tttattttta
     1021 ataatttgcg agtacgcaaa gcttggctgc aggtcgacct cgaggcctcg agttaacgtt
     1081 acgttaacgt taacgttcga ggtcgactct agaatgcata cgcattaagc gaacattaaa
     1141 aagatgtgaa aacataacta ttatgtctaa ataaacacac gtcagatgta tgtacgtcaa
     1201 cggaaaacca ttgtctatat attacaatta ctaaatacat accaaattga atacatattg
     1261 atgaaaaata ataaatactg gcgaaagcaa aaaaacgaaa catttttatt ttattgaaca
     1321 actctcaggc tccaggtagg caaaaaagca ccgactcggt gccacttttt caagttgata
     1381 acggactagc cttattttaa cttgctattt ctagctctaa aacagaagag cctcgctctt
     1441 cccgaagttc acccggatat ctttcctata tatactgtac tcttgcagaa tatgtacttg
     1501 ggaatcataa ggatgcgaag gacgtacgcg ctgtgtctgt aactaagggg ttgctttcgg
     1561 gtacctacca tgccaatgat gggactaccg ccggatagga atcccttttg ggtgcacacc
     1621 gtcacctcca gttccgtctg cctgagttcg gcaatggaaa tcaggtactc aaaggatgca
     1681 tcgtagacgg ggttgcagtt gtccttgatc acgctcgtct tgcgtttcga ctccttggtg
     1741 cgtccaggca acagatacag cttaacatac ggatcgggga tattgctggg atcgcgaagt
     1801 ggtatcttct ggattttgtg tatggtcacg tctagttttt gacgctgggc gctgtagcgg
     1861 atcgacaact gcattcgtcc aaggccatgt tcgccgggtg aggaggtgga gtctggaaag
     1921 cggtgggtca gcacagaggt ggccagctcg ctgatgggtt tttcgctgga ggtggaggcc
     1981 gacatggccg gggagatttt cgtagccgcc acaggatctt cttcactgat gggcgattcg
     2041 gcggacaaac gcttgctgga tgcctgagat tcctaaaagc aatggcaaat tagattcaat
     2101 taaaacaaaa attgtttata taacaaagta aaagaaaaaa aaaaagaatc acagattctc
     2161 cccgacgggg aattgaaccc cggtctcccg cgtgacaggc ggggatacta accactatac
     2221 tatcgaggac gttgaaaatg aattcagatc cagatccact agtggcctaa ttctagtatg
     2281 tatgtaagtt aataaaaccc atttttgcgg aaagtagata aaaaaaacat tttttttttt
     2341 tactgcactg gatatcattg aacttatctg atcagtttta aatttacttc gatccaaggg
     2401 tatttgatgt accaggttct ttcgattacc tctcactcaa aatgacattc cactcaaagt
     2461 cagcgctgtt tgcctccttc tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt
     2521 ccgctatctc tttcgccacc gtttgtagcg ttacgtagcg tcaatgtccg ccttcagttg
     2581 cattttgtca gcggtttcgt gacgaagctc caagcggttt acgccatcaa ttaaacacaa
     2641 agtgctgtgc caaaactcct ctcgcttctt atttttgttt gttttttgag tgattggggt
     2701 ggtgattggt tttgggtggg taagcagggg aaagtgtgaa aaatcccggc aatgggccaa
     2761 gaggatcagg agctattaat tcgcggaggc agcaaacacc catctgccga gcatctgaac
     2821 aatgtgagta gtacatgtgc atacatctta agttcacttg atctatagga actgcgattg
     2881 caacatcaaa ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa accgcaatcg
     2941 cacaaacaaa tagtgacacg aaacagatta ttctggtagc tgtgctcgct atataagaca
     3001 atttttaaga tcatatcatg atcaagacat ctaaaggcat tcattttcga ctacattctt
     3061 ttttacaaaa aatataacaa ccagatattt taagctgatc ctagatgcac aaaaaataaa
     3121 taaaagtata aacctacttc gtaggatact tcgttttgtt cggggttaga tgagcataac
     3181 gcttgtagtt gatatttgag atcccctatc attgcagggt gacagcggag cggcttcgca
     3241 gagctgcatt aaccagggct tcgggcaggc caaaaactac ggcacgctcc tgccacccag
     3301 tccgccggag gactccggtt cagggagcgg ccaactagcc gagaacctca cctatgcctg
     3361 gcacaatatg gacatctttg gggcggtcaa tcagccgggc tccggatggc ggcagctggt
     3421 caaccggaca cgcggactat tctgcaacga gcgacacata ccggcgccca ggaaacattt
     3481 gctcaagaac ggtgagtttc tattcgcagt cggctgatct gtgtgaaatc ttaataaagg
     3541 gtccaattac caatttgaaa ctcagtttgc ggcgtggcct atccgggcga acttttggcc
     3601 gtgatgggca gttccggtgc cggaaagacg accctgctga atgcccttgc ctttcgatcg
     3661 ccgcagggca tccaagtatc gccatccggg atgcgactgc tcaatggcca acctgtggac
     3721 gccaaggaga tgcaggccag gtgcgcctat gtccagcagg atgacctctt tatcggctcc
     3781 ctaacggcca gggaacacct gattttccag gccatggtgc ggatgccacg acatctgacc
     3841 tatcggcagc gagtggcccg cgtggatcag gtgatccagg agctttcgct cagcaaatgt
     3901 cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc tgtccggcgg agaaaggaag
     3961 cgtctggcat tcgcctccga ggcactaacc gatccgccgc ttctgatctg cgatgagccc
     4021 acctccggac tggactcatt taccgcccac agcgtcgtcc aggtgctgaa gaagctgtcg
     4081 cagaagggca agaccgtcat cctgaccatt catcagccgt cttccgagct gtttgagctc
     4141 tttgacaaga tccttctgat ggccgagggc agggtagctt tcttgggcac tcccagcgaa
     4201 gccgtcgact tcttttccta gtgagttcga tgtgtttatt aagggtatct agcattacat
     4261 tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct accaactaca atccggcgga
     4321 cttttacgta caggtgttgg ccgttgtgcc cggacgggag atcgagtccc gtgatcggat
     4381 cgccaagata tgcgacaatt ttgctattag caaagtagcc cgggatatgg agcagttgtt
     4441 ggccaccaaa aatttggaga agccactgga gcagccggag aatgggtaca cctacaaggc
     4501 cacctggttc atgcagttcc gggcggtcct gtggcgatcc tggctgtcgg tgctcaagga
     4561 accactcctc gtaaaagtgc gacttattca gacaacggtg agtggttcca gtggaaacaa
     4621 atgatataac gcttacaatt cttggaaaca aattcgctag attttagtta gaattgcctg
     4681 attccacacc cttcttagtt tttttcaatg agatgtatag tttatagttt tgcagaaaat
     4741 aaataaattt catttaactc gcgaacatgt tgaagatatg aatattaatg agatgcgagt
     4801 aacattttaa tttgcagatg gttgccatct tgattggcct catctttttg ggccaacaac
     4861 tcacgcaagt gggcgtgatg aatatcaacg gagccatctt cctcttcctg accaacatga
     4921 cctttcaaaa cgtctttgcc acgataaatg taagtcttgt ttagaataca tttgcatatt
     4981 aataatttac taactttcta atgaatcgat tcgatttagg tgttcacctc agagctgcca
     5041 gtttttatga gggaggcccg aagtcgactt tatcgctgtg acacatactt tctgggcaaa
     5101 acgattgccg aattaccgct ttttctcaca gtgccactgg tcttcacggc gattgcctat
     5161 ccgatgatcg gactgcgggc cggagtgctg cacttcttca actgcctggc gctggtcact
     5221 ctggtggcca atgtgtcaac gtccttcgga tatctaatat cctgcgccag ctcctcgacc
     5281 tcgatggcgc tgtctgtggg tccgccggtt atcataccat tcctgctctt tggcggcttc
     5341 ttcttgaact cgggctcggt gccagtatac ctcaaatggt tgtcgtacct ctcatggttc
     5401 cgttacgcca acgagggtct gctgattaac caatgggcgg acgtggagcc gggcgaaatt
     5461 agctgcacat cgtcgaacac cacgtgcccc agttcgggca aggtcatcct ggagacgctt
     5521 aacttctccg ccgccgatct gccgctggac tacgtgggtc tggccattct catcgtgagc
     5581 ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga gtagccgaca
     5641 tatatccgaa ataactgctt gttttttttt ttaccattat taccatcgtg tttactgttt
     5701 attgccccct caaaaagcta atgtaattat atttgtgcca ataaaaacaa gatatgacct
     5761 atagaataca agtatttccc cttcgaacat ccccacaagt agactttgga tttgtcttct
     5821 aaccaaaaga cttacacacc tgcatacctt acatcaaaaa ctcgtttatc gctacataaa
     5881 acaccgggat atatttttta tatacatact tttcaaatcg cgcgccctct tcataattca
     5941 cctccaccac accacgtttc gtagttgctc tttcgctgtc tcccacccgc tctccgcaac
     6001 acattcacct tttgttcgac gaccttggag cgactgtcgt tagttccgcg cgattcggtt
     6061 cgctcaaatg gttccgagtg gttcatttcg tctcaataga aattagtaat aaatatttgt
     6121 atgtacaatt tatttgctcc aatatatttg tatatatttc cctcacagct atatttattc
     6181 taatttaata ttatgacttt ttaaggtaat tttttgtgac ctgttcggag tgattagcgt
     6241 tacaatttga actgaaagtg acatccagtg tttgttcctt gtgtagatgc atctcaaaaa
     6301 aatggtgggc ataatagtgt tgtttatata tatcaaaaat aacaactata ataataagaa
     6361 tacatttaat ttagaaaatg cttggatttc actggaacta gaattaattc ggctgctgct
     6421 ctaaacgacg catttcgtac tccaaagtac gaattttttc cctcaagctc ttattttcat
     6481 taaacaatga acaggaccta acgcacagtc acgttattgt ttacataaat gatttttttt
     6541 actattcaaa cttactctgt ttgtgtactc ccactggtat agccttcttt tatcttttct
     6601 ggttcaggct ctatcacttt actaggtacg gcatctgcgt tgagtcgcct ccttttaaat
     6661 gtctgacctt ttgcaggtgc agccttccac tgcgaatcat taaagtgggt atcacaaatt
     6721 tgggagtttt caccaaggct gcacccaagg ctctgctccc acaattttct cttaatagca
     6781 cacttcggca cgtgaattaa ttttactcca gtcacagctt tgcagcaaaa tttgcaatat
     6841 ttcatttttt tttattccac gtaagggtta atgttttcaa aaaaaaattc gtccgcacac
     6901 aacctttcct ctcaacaagc aaacgtgcac tgaatttaag tgtatacttc ggtaagcttc
     6961 ggctatcgac gggaccacct tatgttattt catcatgggc cagacccacg tagtccagcg
     7021 gcagatcggc ggcggagaag ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg
     7081 tggtgttcga cgatgtgcag ctaatttcgc ccggctccac gtccgcccat tggttaatca
     7141 gcagaccctc gttggcgtaa cggaaccatg agaggtacga caaccatttg aggtatactg
     7201 gcaccgagcc cgagttcaag aagaaggcgt ttttccatag gctccgcccc cctgacgagc
     7261 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7321 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7381 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     7441 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7501 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7561 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7621 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7681 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7741 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7801 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7861 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7921 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7981 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8041 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8101 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8161 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8221 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8281 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8341 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8401 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8461 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8521 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8581 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     8641 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8701 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8761 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8821 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8881 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8941 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     9001 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     9061 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     9121 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     9181 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccata
//
